phosphatidate biosynthesis I (the dihydroxyacetone pathway)

gomodel:YeastPathways_PWY3O-6407

Phosphatidate biosynthesis I in Saccharomyces cerevisiae S288C acylates glycerone phosphate to 1-acylglycerone 3-phosphate, reduces it to 1-acyl-sn-glycerol 3-phosphate, and acylates that lysophosphatidate to 1,2-diacyl-sn-glycerol 3-phosphate through the SGD GO-CAM dihydroxyacetone pathway.

Pathway type

lipid biosynthesis

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

24 nodes · 33 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
SCT1 Scer `SGD:S000000107`	protein
phospholipid biosynthetic process `GO:0008654`	biological process
coenzyme A(4-) `CHEBI:57287`	small molecule
SLC1 Scer `SGD:S000002210`	protein
glycerone phosphate(2-) `CHEBI:57642`	small molecule
long-chain fatty acyl-CoA `CHEBI:33184`	lipid
NADPH(4-) `CHEBI:57783`	small molecule
1-acylglycerone 3-phosphate `CHEBI:15835`	small molecule
NADP(3-) `CHEBI:58349`	small molecule
cytosol `GO:0005829`	cellular component
acyl-CoA `CHEBI:17984`	small molecule
hydron `CHEBI:15378`	small molecule
GPT2 Scer `SGD:S000001775`	protein
1-acyl-sn-glycerol 3-phosphate `CHEBI:16975`	lipid
AYR1 Scer `SGD:S000001386`	protein
1,2-diacyl-sn-glycerol 3-phosphate `CHEBI:29089`	lipid
endoplasmic reticulum membrane `GO:0005789`	cellular component
lipid droplet `GO:0005811`	cellular component
mitochondrial outer membrane `GO:0005741`	cellular component
endoplasmic reticulum `GO:0005783`	cellular component
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	molecular activity
glycerone phosphate + a long-chain acyl-CoA -> 1-acyl-dihydroxyacetone-phosphate + coenzyme A `gomodel:RXN3O-5279`	molecular activity
1-acyl-dihydroxyacetone-phosphate + NADPH -> a 1-acyl-sn-glycerol 3-phosphate + NADP⁺ + H⁺ `gomodel:RXN3O-9800`	molecular activity
glycerone-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6407/6a4c244800000335`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
SCT1 Scer `SGD:S000000107`	enables	glycerone-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6407/6a4c244800000335`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6407/6a4c244800000335 RO:0002333 gomodel:YeastPathways_PWY3O-6407/6a4c244800000339. Source location `./YeastPathways_PWY3O-6407.json#/facts/0; endpoint types: /individuals/40/type; /individuals/44/type`
1-acyl-dihydroxyacetone-phosphate + NADPH -> a 1-acyl-sn-glycerol 3-phosphate + NADP⁺ + H⁺ `gomodel:RXN3O-9800`	part_of	phospholipid biosynthetic process `GO:0008654`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-9800 BFO:0000050 gomodel:YeastPathways_PWY3O-6407/YeastPathways_PWY3O-6407. Source location `./YeastPathways_PWY3O-6407.json#/facts/1; endpoint types: /individuals/14/type; /individuals/39/type`
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	has_output	coenzyme A(4-) `CHEBI:57287`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN-1623 RO:0002234 gomodel:CHEBI_57287_RXN-1623. Source location `./YeastPathways_PWY3O-6407.json#/facts/2; endpoint types: /individuals/12/type; /individuals/6/type`
glycerone phosphate + a long-chain acyl-CoA -> 1-acyl-dihydroxyacetone-phosphate + coenzyme A `gomodel:RXN3O-5279`	has_output	coenzyme A(4-) `CHEBI:57287`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-5279 RO:0002234 gomodel:CHEBI_57287_RXN3O-5279. Source location `./YeastPathways_PWY3O-6407.json#/facts/3; endpoint types: /individuals/13/type; /individuals/7/type`
glycerone-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6407/6a4c244800000335`	has_output	coenzyme A(4-) `CHEBI:57287`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6407/6a4c244800000335 RO:0002234 gomodel:YeastPathways_PWY3O-6407/6a4c244800000338. Source location `./YeastPathways_PWY3O-6407.json#/facts/4; endpoint types: /individuals/40/type; /individuals/43/type`
SLC1 Scer `SGD:S000002210`	enables	an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN-1623 RO:0002333 gomodel:YDL052C-MONOMER_RXN-1623_controller. Source location `./YeastPathways_PWY3O-6407.json#/facts/5; endpoint types: /individuals/12/type; /individuals/15/type`
glycerone-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6407/6a4c244800000335`	has_input	glycerone phosphate(2-) `CHEBI:57642`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6407/6a4c244800000335 RO:0002233 gomodel:YeastPathways_PWY3O-6407/6a4c244800000337. Source location `./YeastPathways_PWY3O-6407.json#/facts/6; endpoint types: /individuals/40/type; /individuals/42/type`
glycerone-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6407/6a4c244800000335`	has_input	long-chain fatty acyl-CoA `CHEBI:33184`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6407/6a4c244800000335 RO:0002233 gomodel:YeastPathways_PWY3O-6407/6a4c244800000336. Source location `./YeastPathways_PWY3O-6407.json#/facts/7; endpoint types: /individuals/40/type; /individuals/41/type`
1-acyl-dihydroxyacetone-phosphate + NADPH -> a 1-acyl-sn-glycerol 3-phosphate + NADP⁺ + H⁺ `gomodel:RXN3O-9800`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-9800 RO:0002233 gomodel:CHEBI_57783_RXN3O-9800. Source location `./YeastPathways_PWY3O-6407.json#/facts/8; endpoint types: /individuals/14/type; /individuals/9/type`
glycerone-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6407/6a4c244800000335`	has_output	1-acylglycerone 3-phosphate `CHEBI:15835`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6407/6a4c244800000335 RO:0002234 gomodel:CHEBI_15835_RXN3O-5279. Source location `./YeastPathways_PWY3O-6407.json#/facts/9; endpoint types: /individuals/40/type; /individuals/1/type`
1-acyl-dihydroxyacetone-phosphate + NADPH -> a 1-acyl-sn-glycerol 3-phosphate + NADP⁺ + H⁺ `gomodel:RXN3O-9800`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-9800 RO:0002234 gomodel:CHEBI_58349_RXN3O-9800. Source location `./YeastPathways_PWY3O-6407.json#/facts/10; endpoint types: /individuals/14/type; /individuals/10/type`
1-acyl-dihydroxyacetone-phosphate + NADPH -> a 1-acyl-sn-glycerol 3-phosphate + NADP⁺ + H⁺ `gomodel:RXN3O-9800`	has_input	1-acylglycerone 3-phosphate `CHEBI:15835`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-9800 RO:0002233 gomodel:CHEBI_15835_RXN3O-5279. Source location `./YeastPathways_PWY3O-6407.json#/facts/11; endpoint types: /individuals/14/type; /individuals/1/type`
glycerone-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6407/6a4c244800000335`	part_of	phospholipid biosynthetic process `GO:0008654`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6407/6a4c244800000335 BFO:0000050 gomodel:YeastPathways_PWY3O-6407/YeastPathways_PWY3O-6407. Source location `./YeastPathways_PWY3O-6407.json#/facts/13; endpoint types: /individuals/40/type; /individuals/39/type`
glycerone phosphate + a long-chain acyl-CoA -> 1-acyl-dihydroxyacetone-phosphate + coenzyme A `gomodel:RXN3O-5279`	has_input	long-chain fatty acyl-CoA `CHEBI:33184`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-5279 RO:0002233 gomodel:CHEBI_33184_RXN3O-5279. Source location `./YeastPathways_PWY3O-6407.json#/facts/15; endpoint types: /individuals/13/type; /individuals/5/type`
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	has_input	acyl-CoA `CHEBI:17984`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN-1623 RO:0002233 gomodel:CHEBI_17984_RXN-1623. Source location `./YeastPathways_PWY3O-6407.json#/facts/16; endpoint types: /individuals/12/type; /individuals/3/type`
1-acyl-dihydroxyacetone-phosphate + NADPH -> a 1-acyl-sn-glycerol 3-phosphate + NADP⁺ + H⁺ `gomodel:RXN3O-9800`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-9800 RO:0002234 gomodel:CHEBI_15378_RXN3O-9800. Source location `./YeastPathways_PWY3O-6407.json#/facts/18; endpoint types: /individuals/14/type; /individuals/0/type`
GPT2 Scer `SGD:S000001775`	enables	glycerone phosphate + a long-chain acyl-CoA -> 1-acyl-dihydroxyacetone-phosphate + coenzyme A `gomodel:RXN3O-5279`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-5279 RO:0002333 gomodel:MONOMER3O-4095_RXN3O-5279_controller. Source location `./YeastPathways_PWY3O-6407.json#/facts/19; endpoint types: /individuals/13/type; /individuals/11/type`
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	has_input	1-acyl-sn-glycerol 3-phosphate `CHEBI:16975`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN-1623 RO:0002233 gomodel:CHEBI_16975_RXN3O-9800. Source location `./YeastPathways_PWY3O-6407.json#/facts/21; endpoint types: /individuals/12/type; /individuals/2/type`
AYR1 Scer `SGD:S000001386`	enables	1-acyl-dihydroxyacetone-phosphate + NADPH -> a 1-acyl-sn-glycerol 3-phosphate + NADP⁺ + H⁺ `gomodel:RXN3O-9800`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-9800 RO:0002333 gomodel:YIL124W-MONOMER_RXN3O-9800_controller. Source location `./YeastPathways_PWY3O-6407.json#/facts/22; endpoint types: /individuals/14/type; /individuals/16/type`
glycerone phosphate + a long-chain acyl-CoA -> 1-acyl-dihydroxyacetone-phosphate + coenzyme A `gomodel:RXN3O-5279`	has_input	glycerone phosphate(2-) `CHEBI:57642`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-5279 RO:0002233 gomodel:CHEBI_57642_RXN3O-5279. Source location `./YeastPathways_PWY3O-6407.json#/facts/23; endpoint types: /individuals/13/type; /individuals/8/type`
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	part_of	phospholipid biosynthetic process `GO:0008654`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN-1623 BFO:0000050 gomodel:YeastPathways_PWY3O-6407/YeastPathways_PWY3O-6407. Source location `./YeastPathways_PWY3O-6407.json#/facts/24; endpoint types: /individuals/12/type; /individuals/39/type`
glycerone phosphate + a long-chain acyl-CoA -> 1-acyl-dihydroxyacetone-phosphate + coenzyme A `gomodel:RXN3O-5279`	has_output	1-acylglycerone 3-phosphate `CHEBI:15835`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-5279 RO:0002234 gomodel:CHEBI_15835_RXN3O-5279. Source location `./YeastPathways_PWY3O-6407.json#/facts/25; endpoint types: /individuals/13/type; /individuals/1/type`
glycerone phosphate + a long-chain acyl-CoA -> 1-acyl-dihydroxyacetone-phosphate + coenzyme A `gomodel:RXN3O-5279`	part_of	phospholipid biosynthetic process `GO:0008654`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-5279 BFO:0000050 gomodel:YeastPathways_PWY3O-6407/YeastPathways_PWY3O-6407. Source location `./YeastPathways_PWY3O-6407.json#/facts/26; endpoint types: /individuals/13/type; /individuals/39/type`
1-acyl-dihydroxyacetone-phosphate + NADPH -> a 1-acyl-sn-glycerol 3-phosphate + NADP⁺ + H⁺ `gomodel:RXN3O-9800`	has_output	1-acyl-sn-glycerol 3-phosphate `CHEBI:16975`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN3O-9800 RO:0002234 gomodel:CHEBI_16975_RXN3O-9800. Source location `./YeastPathways_PWY3O-6407.json#/facts/27; endpoint types: /individuals/14/type; /individuals/2/type`
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	has_output	1,2-diacyl-sn-glycerol 3-phosphate `CHEBI:29089`	gomodel:YeastPathways_PWY3O-6407 Source assertion: The model asserts gomodel:RXN-1623 RO:0002234 gomodel:CHEBI_29089_RXN-1623. Source location `./YeastPathways_PWY3O-6407.json#/facts/28; endpoint types: /individuals/12/type; /individuals/4/type`
SCT1 Scer `SGD:S000000107`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:19525420) Topology: Multi-pass membrane protein. Source qualification: Localizes to both perinuclear and cortical endoplasmic reticulum	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P32784 Source assertion: UniProtKB:P32784 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/995/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P32784 Source assertion: UniProtKB:P32784 has the exact SGD cross-reference SGD:S000000107. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/995/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
SLC1 Scer `SGD:S000002210`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:18154737); ECO:0000269 (PubMed:21820081); ECO:0000269 (PubMed:24868093)	lipid droplet `GO:0005811`	UniProtKB:P33333 Source assertion: UniProtKB:P33333 annotates location Lipid droplet (SL-0154). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1078/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P33333 Source assertion: UniProtKB:P33333 has the exact SGD cross-reference SGD:S000002210. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1078/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
GPT2 Scer `SGD:S000001775`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:22267742); ECO:0000269 (PubMed:31421179); ECO:0000269 (PubMed:9401016) Source qualification: Localizes to both perinuclear and cortical endoplasmic reticulum	lipid droplet `GO:0005811`	UniProtKB:P36148 Source assertion: UniProtKB:P36148 annotates location Lipid droplet (SL-0154). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1237/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P36148 Source assertion: UniProtKB:P36148 has the exact SGD cross-reference SGD:S000001775. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1237/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
GPT2 Scer `SGD:S000001775`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:19525420); ECO:0000269 (PubMed:31421179) Topology: Multi-pass membrane protein. Source qualification: Localizes to both perinuclear and cortical endoplasmic reticulum	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P36148 Source assertion: UniProtKB:P36148 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1237/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P36148 Source assertion: UniProtKB:P36148 has the exact SGD cross-reference SGD:S000001775. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1237/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
AYR1 Scer `SGD:S000001386`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:10515935); ECO:0000269 (PubMed:10617610); ECO:0000269 (PubMed:21820081); ECO:0000269 (PubMed:24868093)	lipid droplet `GO:0005811`	UniProtKB:P40471 Source assertion: UniProtKB:P40471 annotates location Lipid droplet (SL-0154). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1727/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P40471 Source assertion: UniProtKB:P40471 has the exact SGD cross-reference SGD:S000001386. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1727/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
AYR1 Scer `SGD:S000001386`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:16407407); ECO:0000269 (PubMed:28916712)	mitochondrial outer membrane `GO:0005741`	UniProtKB:P40471 Source assertion: UniProtKB:P40471 annotates location Mitochondrion outer membrane (SL-0172). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1727/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P40471 Source assertion: UniProtKB:P40471 has the exact SGD cross-reference SGD:S000001386. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1727/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
AYR1 Scer `SGD:S000001386`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:28916712)	endoplasmic reticulum `GO:0005783`	UniProtKB:P40471 Source assertion: UniProtKB:P40471 annotates location Endoplasmic reticulum (SL-0095). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1727/comments/1/subcellularLocations/2; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P40471 Source assertion: UniProtKB:P40471 has the exact SGD cross-reference SGD:S000001386. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1727/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	occurs_in Activity compartment supported by an inspected functional annotation. ECO:0000269 (PubMed:17675291); ECO:0000269 (PubMed:17890783); ECO:0000269 (PubMed:20694142); ECO:0000269 (PubMed:9401016). Alternative isoenzymes may function elsewhere.	lipid droplet `GO:0005811`	UniProtKB:P33333 Source assertion: UniProtKB:P33333 describes the corresponding catalytic function in the lipid droplet; this projection applies to the activity enabled by SGD:S000002210. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1078/comments/0/texts/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`

References

gomodel:YeastPathways_PWY3O-6407 SGD GO-CAM phosphatidate biosynthesis I (the dihydroxyacetone pathway) - imported from: Saccharomyces Genome Database (source version: Pinned Noctua JSON archive; model date 2026-07-07; member ./YeastPathways_PWY3O-6407.json)
UniProtKB:P32784 Reviewed UniProtKB P32784 location annotations (source version: UniProt release 2026_03)
UniProtKB:P33333 Reviewed UniProtKB P33333 location annotations (source version: UniProt release 2026_03)
UniProtKB:P36148 Reviewed UniProtKB P36148 location annotations (source version: UniProt release 2026_03)
UniProtKB:P40471 Reviewed UniProtKB P40471 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 4 activity location assignments and 4 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 8 independently supported location edges and quarantined 4 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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