phosphatidate biosynthesis II (the glycerol-3-phosphate pathway)

gomodel:YeastPathways_PWY3O-6499

Phosphatidate biosynthesis II in Saccharomyces cerevisiae S288C forms sn-glycerol 3-phosphate, acylates it to 1-acyl-sn-glycerol 3-phosphate, and acylates that lysophosphatidate to 1,2-diacyl-sn-glycerol 3-phosphate through the SGD GO-CAM glycerol-3-phosphate pathway.

Pathway type

lipid biosynthesis

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

27 nodes · 43 asserted directed edges

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Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
SCT1 Scer `SGD:S000000107`	protein
GPD2 Scer `SGD:S000005420`	protein
phospholipid biosynthetic process `GO:0008654`	biological process
coenzyme A(4-) `CHEBI:57287`	small molecule
glycerone phosphate(2-) `CHEBI:57642`	small molecule
long-chain fatty acyl-CoA `CHEBI:33184`	lipid
1,2-diacyl-sn-glycerol 3-phosphate `CHEBI:29089`	lipid
1-acyl-sn-glycerol 3-phosphate `CHEBI:16975`	lipid
cytosol `GO:0005829`	cellular component
GPT2 Scer `SGD:S000001775`	protein
GPD1 Scer `SGD:S000002180`	protein
sn-glycerol 3-phosphate(2-) `CHEBI:57597`	small molecule
acyl-CoA `CHEBI:17984`	small molecule
hydron `CHEBI:15378`	small molecule
NAD(1-) `CHEBI:57540`	small molecule
NADH(2-) `CHEBI:57945`	small molecule
SLC1 Scer `SGD:S000002210`	protein
endoplasmic reticulum membrane `GO:0005789`	cellular component
cytoplasm `GO:0005737`	cellular component
mitochondrion `GO:0005739`	cellular component
lipid droplet `GO:0005811`	cellular component
peroxisome `GO:0005777`	cellular component
sn-glycerol 3-phosphate + NAD⁺ <- glycerone phosphate + NADH + H⁺ `gomodel:1.1.1.8-RXN`	molecular activity
sn-glycerol 3-phosphate + a long-chain acyl-CoA -> a 1-acyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN`	molecular activity
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	molecular activity
glycerol-3-phosphate dehydrogenase [NAD(P)+] activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001520`	molecular activity
glycerol-3-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001536`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
SCT1 Scer `SGD:S000000107`	enables	glycerol-3-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001536`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001536 RO:0002333 gomodel:YeastPathways_PWY3O-6499/6a4c244800001540. Source location `./YeastPathways_PWY3O-6499.json#/facts/0; endpoint types: /individuals/55/type; /individuals/58/type`
GPD2 Scer `SGD:S000005420`	enables	glycerol-3-phosphate dehydrogenase [NAD(P)+] activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001520`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001520 RO:0002333 gomodel:YeastPathways_PWY3O-6499/6a4c244800001526. Source location `./YeastPathways_PWY3O-6499.json#/facts/1; endpoint types: /individuals/40/type; /individuals/45/type`
sn-glycerol 3-phosphate + NAD⁺ <- glycerone phosphate + NADH + H⁺ `gomodel:1.1.1.8-RXN`	part_of	phospholipid biosynthetic process `GO:0008654`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:1.1.1.8-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-6499/YeastPathways_PWY3O-6499. Source location `./YeastPathways_PWY3O-6499.json#/facts/2; endpoint types: /individuals/38/type; /individuals/39/type`
sn-glycerol 3-phosphate + a long-chain acyl-CoA -> a 1-acyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN`	has_output	coenzyme A(4-) `CHEBI:57287`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN RO:0002234 gomodel:CHEBI_57287_GLYCEROL-3-P-ACYLTRANSFER-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/3; endpoint types: /individuals/11/type; /individuals/5/type`
sn-glycerol 3-phosphate + NAD⁺ <- glycerone phosphate + NADH + H⁺ `gomodel:1.1.1.8-RXN`	has_input	glycerone phosphate(2-) `CHEBI:57642`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:1.1.1.8-RXN RO:0002233 gomodel:CHEBI_57642_1.1.1.8-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/4; endpoint types: /individuals/38/type; /individuals/9/type`
glycerol-3-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001536`	has_input	long-chain fatty acyl-CoA `CHEBI:33184`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001536 RO:0002233 gomodel:YeastPathways_PWY3O-6499/6a4c244800001537. Source location `./YeastPathways_PWY3O-6499.json#/facts/5; endpoint types: /individuals/55/type; /individuals/56/type`
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	has_output	1,2-diacyl-sn-glycerol 3-phosphate `CHEBI:29089`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:RXN-1623 RO:0002234 gomodel:CHEBI_29089_RXN-1623. Source location `./YeastPathways_PWY3O-6499.json#/facts/6; endpoint types: /individuals/13/type; /individuals/3/type`
glycerol-3-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001536`	has_output	coenzyme A(4-) `CHEBI:57287`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001536 RO:0002234 gomodel:YeastPathways_PWY3O-6499/6a4c244800001538. Source location `./YeastPathways_PWY3O-6499.json#/facts/7; endpoint types: /individuals/55/type; /individuals/57/type`
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	part_of	phospholipid biosynthetic process `GO:0008654`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:RXN-1623 BFO:0000050 gomodel:YeastPathways_PWY3O-6499/YeastPathways_PWY3O-6499. Source location `./YeastPathways_PWY3O-6499.json#/facts/8; endpoint types: /individuals/13/type; /individuals/39/type`
glycerol-3-phosphate dehydrogenase [NAD(P)+] activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001520`	part_of	phospholipid biosynthetic process `GO:0008654`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001520 BFO:0000050 gomodel:YeastPathways_PWY3O-6499/YeastPathways_PWY3O-6499. Source location `./YeastPathways_PWY3O-6499.json#/facts/9; endpoint types: /individuals/40/type; /individuals/39/type`
sn-glycerol 3-phosphate + a long-chain acyl-CoA -> a 1-acyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN`	has_output	1-acyl-sn-glycerol 3-phosphate `CHEBI:16975`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN RO:0002234 gomodel:CHEBI_16975_GLYCEROL-3-P-ACYLTRANSFER-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/10; endpoint types: /individuals/11/type; /individuals/1/type`
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	has_output	coenzyme A(4-) `CHEBI:57287`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:RXN-1623 RO:0002234 gomodel:CHEBI_57287_RXN-1623. Source location `./YeastPathways_PWY3O-6499.json#/facts/12; endpoint types: /individuals/13/type; /individuals/6/type`
glycerol-3-phosphate dehydrogenase [NAD(P)+] activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001520`	has_input	glycerone phosphate(2-) `CHEBI:57642`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001520 RO:0002233 gomodel:YeastPathways_PWY3O-6499/6a4c244800001521. Source location `./YeastPathways_PWY3O-6499.json#/facts/13; endpoint types: /individuals/40/type; /individuals/41/type`
GPT2 Scer `SGD:S000001775`	enables	sn-glycerol 3-phosphate + a long-chain acyl-CoA -> a 1-acyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN RO:0002333 gomodel:MONOMER3O-4095_GLYCEROL-3-P-ACYLTRANSFER-RXN_controller. Source location `./YeastPathways_PWY3O-6499.json#/facts/15; endpoint types: /individuals/11/type; /individuals/12/type`
GPD1 Scer `SGD:S000002180`	enables	sn-glycerol 3-phosphate + NAD⁺ <- glycerone phosphate + NADH + H⁺ `gomodel:1.1.1.8-RXN`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:1.1.1.8-RXN RO:0002333 gomodel:YDL022W-MONOMER_1.1.1.8-RXN_controller. Source location `./YeastPathways_PWY3O-6499.json#/facts/16; endpoint types: /individuals/38/type; /individuals/14/type`
sn-glycerol 3-phosphate + a long-chain acyl-CoA -> a 1-acyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN`	has_input	sn-glycerol 3-phosphate(2-) `CHEBI:57597`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN RO:0002233 gomodel:CHEBI_57597_1.1.1.8-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/17; endpoint types: /individuals/11/type; /individuals/8/type`
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	has_input	1-acyl-sn-glycerol 3-phosphate `CHEBI:16975`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:RXN-1623 RO:0002233 gomodel:CHEBI_16975_GLYCEROL-3-P-ACYLTRANSFER-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/19; endpoint types: /individuals/13/type; /individuals/1/type`
glycerol-3-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001536`	part_of	phospholipid biosynthetic process `GO:0008654`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001536 BFO:0000050 gomodel:YeastPathways_PWY3O-6499/YeastPathways_PWY3O-6499. Source location `./YeastPathways_PWY3O-6499.json#/facts/20; endpoint types: /individuals/55/type; /individuals/39/type`
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	has_input	acyl-CoA `CHEBI:17984`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:RXN-1623 RO:0002233 gomodel:CHEBI_17984_RXN-1623. Source location `./YeastPathways_PWY3O-6499.json#/facts/21; endpoint types: /individuals/13/type; /individuals/2/type`
glycerol-3-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001536`	has_output	1-acyl-sn-glycerol 3-phosphate `CHEBI:16975`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001536 RO:0002234 gomodel:CHEBI_16975_GLYCEROL-3-P-ACYLTRANSFER-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/22; endpoint types: /individuals/55/type; /individuals/1/type`
sn-glycerol 3-phosphate + NAD⁺ <- glycerone phosphate + NADH + H⁺ `gomodel:1.1.1.8-RXN`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:1.1.1.8-RXN RO:0002233 gomodel:CHEBI_15378_1.1.1.8-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/23; endpoint types: /individuals/38/type; /individuals/0/type`
glycerol-3-phosphate dehydrogenase [NAD(P)+] activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001520`	has_output	sn-glycerol 3-phosphate(2-) `CHEBI:57597`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001520 RO:0002234 gomodel:CHEBI_57597_1.1.1.8-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/24; endpoint types: /individuals/40/type; /individuals/8/type`
sn-glycerol 3-phosphate + NAD⁺ <- glycerone phosphate + NADH + H⁺ `gomodel:1.1.1.8-RXN`	has_output	NAD(1-) `CHEBI:57540`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:1.1.1.8-RXN RO:0002234 gomodel:CHEBI_57540_1.1.1.8-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/25; endpoint types: /individuals/38/type; /individuals/7/type`
sn-glycerol 3-phosphate + a long-chain acyl-CoA -> a 1-acyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN`	has_input	long-chain fatty acyl-CoA `CHEBI:33184`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN RO:0002233 gomodel:CHEBI_33184_GLYCEROL-3-P-ACYLTRANSFER-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/26; endpoint types: /individuals/11/type; /individuals/4/type`
glycerol-3-phosphate dehydrogenase [NAD(P)+] activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001520`	has_output	NAD(1-) `CHEBI:57540`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001520 RO:0002234 gomodel:YeastPathways_PWY3O-6499/6a4c244800001524. Source location `./YeastPathways_PWY3O-6499.json#/facts/27; endpoint types: /individuals/40/type; /individuals/44/type`
sn-glycerol 3-phosphate + NAD⁺ <- glycerone phosphate + NADH + H⁺ `gomodel:1.1.1.8-RXN`	has_input	NADH(2-) `CHEBI:57945`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:1.1.1.8-RXN RO:0002233 gomodel:CHEBI_57945_1.1.1.8-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/28; endpoint types: /individuals/38/type; /individuals/10/type`
SLC1 Scer `SGD:S000002210`	enables	an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:RXN-1623 RO:0002333 gomodel:YDL052C-MONOMER_RXN-1623_controller. Source location `./YeastPathways_PWY3O-6499.json#/facts/29; endpoint types: /individuals/13/type; /individuals/15/type`
glycerol-3-phosphate dehydrogenase [NAD(P)+] activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001520`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001520 RO:0002233 gomodel:YeastPathways_PWY3O-6499/6a4c244800001522. Source location `./YeastPathways_PWY3O-6499.json#/facts/30; endpoint types: /individuals/40/type; /individuals/42/type`
sn-glycerol 3-phosphate + NAD⁺ <- glycerone phosphate + NADH + H⁺ `gomodel:1.1.1.8-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:1.1.1.8-RXN BFO:0000066 gomodel:reaction_1.1.1.8-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY3O-6499.json#/facts/31; endpoint types: /individuals/38/type; /individuals/35/type`
glycerol-3-phosphate dehydrogenase [NAD(P)+] activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001520`	has_input	NADH(2-) `CHEBI:57945`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001520 RO:0002233 gomodel:YeastPathways_PWY3O-6499/6a4c244800001523. Source location `./YeastPathways_PWY3O-6499.json#/facts/32; endpoint types: /individuals/40/type; /individuals/43/type`
sn-glycerol 3-phosphate + NAD⁺ <- glycerone phosphate + NADH + H⁺ `gomodel:1.1.1.8-RXN`	has_output	sn-glycerol 3-phosphate(2-) `CHEBI:57597`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:1.1.1.8-RXN RO:0002234 gomodel:CHEBI_57597_1.1.1.8-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/33; endpoint types: /individuals/38/type; /individuals/8/type`
glycerol-3-phosphate O-acyltransferase activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001536`	has_input	sn-glycerol 3-phosphate(2-) `CHEBI:57597`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001536 RO:0002233 gomodel:CHEBI_57597_1.1.1.8-RXN. Source location `./YeastPathways_PWY3O-6499.json#/facts/34; endpoint types: /individuals/55/type; /individuals/8/type`
glycerol-3-phosphate dehydrogenase [NAD(P)+] activity `gomodel:YeastPathways_PWY3O-6499/6a4c244800001520`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-6499/6a4c244800001520 BFO:0000066 gomodel:YeastPathways_PWY3O-6499/6a4c244800001527. Source location `./YeastPathways_PWY3O-6499.json#/facts/35; endpoint types: /individuals/40/type; /individuals/46/type`
sn-glycerol 3-phosphate + a long-chain acyl-CoA -> a 1-acyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN`	part_of	phospholipid biosynthetic process `GO:0008654`	gomodel:YeastPathways_PWY3O-6499 Source assertion: The model asserts gomodel:GLYCEROL-3-P-ACYLTRANSFER-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-6499/YeastPathways_PWY3O-6499. Source location `./YeastPathways_PWY3O-6499.json#/facts/36; endpoint types: /individuals/11/type; /individuals/39/type`
SCT1 Scer `SGD:S000000107`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:19525420) Topology: Multi-pass membrane protein. Source qualification: Localizes to both perinuclear and cortical endoplasmic reticulum	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P32784 Source assertion: UniProtKB:P32784 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/995/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P32784 Source assertion: UniProtKB:P32784 has the exact SGD cross-reference SGD:S000000107. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/995/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
GPD2 Scer `SGD:S000005420`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	cytoplasm `GO:0005737`	UniProtKB:P41911 Source assertion: UniProtKB:P41911 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1828/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P41911 Source assertion: UniProtKB:P41911 has the exact SGD cross-reference SGD:S000005420. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1828/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
GPD2 Scer `SGD:S000005420`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	mitochondrion `GO:0005739`	UniProtKB:P41911 Source assertion: UniProtKB:P41911 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1828/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P41911 Source assertion: UniProtKB:P41911 has the exact SGD cross-reference SGD:S000005420. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1828/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
GPT2 Scer `SGD:S000001775`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:22267742); ECO:0000269 (PubMed:31421179); ECO:0000269 (PubMed:9401016) Source qualification: Localizes to both perinuclear and cortical endoplasmic reticulum	lipid droplet `GO:0005811`	UniProtKB:P36148 Source assertion: UniProtKB:P36148 annotates location Lipid droplet (SL-0154). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1237/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P36148 Source assertion: UniProtKB:P36148 has the exact SGD cross-reference SGD:S000001775. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1237/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
GPT2 Scer `SGD:S000001775`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:19525420); ECO:0000269 (PubMed:31421179) Topology: Multi-pass membrane protein. Source qualification: Localizes to both perinuclear and cortical endoplasmic reticulum	endoplasmic reticulum membrane `GO:0005789`	UniProtKB:P36148 Source assertion: UniProtKB:P36148 annotates location Endoplasmic reticulum membrane (SL-0097). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1237/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P36148 Source assertion: UniProtKB:P36148 has the exact SGD cross-reference SGD:S000001775. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1237/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
GPD1 Scer `SGD:S000002180`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:15210723)	cytoplasm `GO:0005737`	UniProtKB:Q00055 Source assertion: UniProtKB:Q00055 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2362/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q00055 Source assertion: UniProtKB:Q00055 has the exact SGD cross-reference SGD:S000002180. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2362/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
GPD1 Scer `SGD:S000002180`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:15210723)	peroxisome `GO:0005777`	UniProtKB:Q00055 Source assertion: UniProtKB:Q00055 annotates location Peroxisome (SL-0204). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2362/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q00055 Source assertion: UniProtKB:Q00055 has the exact SGD cross-reference SGD:S000002180. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2362/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
SLC1 Scer `SGD:S000002210`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:18154737); ECO:0000269 (PubMed:21820081); ECO:0000269 (PubMed:24868093)	lipid droplet `GO:0005811`	UniProtKB:P33333 Source assertion: UniProtKB:P33333 annotates location Lipid droplet (SL-0154). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1078/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P33333 Source assertion: UniProtKB:P33333 has the exact SGD cross-reference SGD:S000002210. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1078/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
an acyl-CoA + a 1-acyl-sn-glycerol 3-phosphate -> a 1,2-diacyl-sn-glycerol 3-phosphate + coenzyme A `gomodel:RXN-1623`	occurs_in Activity compartment supported by an inspected functional annotation. ECO:0000269 (PubMed:17675291); ECO:0000269 (PubMed:17890783); ECO:0000269 (PubMed:20694142); ECO:0000269 (PubMed:9401016). Alternative isoenzymes may function elsewhere.	lipid droplet `GO:0005811`	UniProtKB:P33333 Source assertion: UniProtKB:P33333 describes the corresponding catalytic function in the lipid droplet; this projection applies to the activity enabled by SGD:S000002210. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1078/comments/0/texts/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`

References

gomodel:YeastPathways_PWY3O-6499 SGD GO-CAM phosphatidate biosynthesis II (the glycerol-3-phosphate pathway) - imported from: Saccharomyces Genome Database (source version: Pinned Noctua JSON archive; model date 2026-07-13; member ./YeastPathways_PWY3O-6499.json)
UniProtKB:P32784 Reviewed UniProtKB P32784 location annotations (source version: UniProt release 2026_03)
UniProtKB:P41911 Reviewed UniProtKB P41911 location annotations (source version: UniProt release 2026_03)
UniProtKB:P36148 Reviewed UniProtKB P36148 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q00055 Reviewed UniProtKB Q00055 location annotations (source version: UniProt release 2026_03)
UniProtKB:P33333 Reviewed UniProtKB P33333 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 5 activity location assignments and 5 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 9 independently supported location edges and quarantined 3 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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