folate interconversions

gomodel:YeastPathways_PWY3O-697

Folate interconversions in Saccharomyces cerevisiae S288C interconvert tetrahydrofolate-derived one-carbon carriers with formate, serine/glycine, thymidylate synthase, and dihydrofolate reductase reactions in the SGD GO-CAM folate cycle.

Pathway type

folate metabolism

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

59 nodes · 138 asserted directed edges

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
5,10-methylenetetrahydrofolic acid `CHEBI:20502`	small molecule
folic acid metabolic process `GO:0046655`	biological process
mitochondrion `GO:0005739`	cellular component
hydron `CHEBI:15378`	small molecule
tetrahydrofolate `CHEBI:67016`	small molecule
dTMP(2-) `CHEBI:63528`	small molecule
glycine zwitterion `CHEBI:57305`	small molecule
NAD(1-) `CHEBI:57540`	small molecule
5-methyltetrahydrofolate `CHEBI:20612`	small molecule
cytosol `GO:0005829`	cellular component
formate `CHEBI:15740`	small molecule
10-formyltetrahydrofolyl-(Glu)n polyanion `CHEBI:134413`	participant
cytoplasm `GO:0005737`	cellular component
L-serine zwitterion `CHEBI:33384`	small molecule
MIS1 Scer `SGD:S000000288`	protein
ADE3 Scer `SGD:S000003436`	protein
water `CHEBI:15377`	small molecule
MTD1 Scer `SGD:S000001788`	protein
hydrogenphosphate `CHEBI:43474`	small molecule
CPX-1268 Scer `SGD:S000218158`	complex
MET12 Scer `SGD:S000005944`	protein
ATP(4-) `CHEBI:30616`	small molecule
DFR1 Scer `SGD:S000005762`	protein
MET13 Scer `SGD:S000003093`	protein
dihydrofolate(2-) `CHEBI:57451`	small molecule
NADP(3-) `CHEBI:58349`	small molecule
SHM2 Scer `SGD:S000004048`	protein
ammonium `CHEBI:28938`	small molecule
NADPH(4-) `CHEBI:57783`	small molecule
NADH(2-) `CHEBI:57945`	small molecule
carbon dioxide `CHEBI:16526`	small molecule
SHM1 Scer `SGD:S000000467`	protein
ADP(3-) `CHEBI:456216`	small molecule
CDC21 Scer `SGD:S000005600`	protein
dUMP(2-) `CHEBI:246422`	small molecule
Glycine cleavage system H protein, mitochondrial `UniProtKB:P39726`	protein
FAD(3-) `CHEBI:57692`	cofactor
LPD1 Scer `SGD:S000001876`	protein
GCV2 Scer `SGD:S000004801`	protein
GCV1 Scer `SGD:S000002426`	protein
nucleus `GO:0005634`	cellular component
mitochondrial matrix `GO:0005759`	cellular component
(6R)-5,10-methenyltetrahydrofolate `CHEBI:57455`	small molecule
pyridoxal 5'-phosphate(2-) `CHEBI:597326`	cofactor
(R)-lipoate `CHEBI:83088`	cofactor
a 5,10-methylenetetrahydrofolate + NAD⁺ -> a 5,10-methenyltetrahydrofolate + NADH `gomodel:1.5.1.15-RXN`	molecular activity
a 5-methyltetrahydrofolate + NAD⁺ <- a 5,10-methylenetetrahydrofolate + NADH + H⁺ `gomodel:1.5.1.20-RXN`	molecular activity
a tetrahydrofolate + NADP⁺ <- a 7,8-dihydrofolate + NADPH + H⁺ `gomodel:DIHYDROFOLATEREDUCT-RXN`	molecular activity
a tetrahydrofolate + ATP + formate <=> a 10-formyltetrahydrofolate + ADP + phosphate `gomodel:FORMATETHFLIG-RXN`	molecular activity
glycine + a tetrahydrofolate + NAD⁺ -> a 5,10-methylenetetrahydrofolate + ammonium + CO₂ + NADH `gomodel:GCVMULTI-RXN`	molecular activity
L-serine + a tetrahydrofolate <=> glycine + a 5,10-methylenetetrahydrofolate + H₂O `gomodel:GLYOHMETRANS-RXN`	molecular activity
a 5,10-methenyltetrahydrofolate + H₂O <=> a 10-formyltetrahydrofolate + H⁺ `gomodel:METHENYLTHFCYCLOHYDRO-RXN`	molecular activity
a 5,10-methylenetetrahydrofolate + NADP⁺ <=> a 5,10-methenyltetrahydrofolate + NADPH `gomodel:METHYLENETHFDEHYDROG-NADP-RXN`	molecular activity
a 5,10-methylenetetrahydrofolate + dUMP -> dTMP + a 7,8-dihydrofolate `gomodel:THYMIDYLATESYN-RXN`	molecular activity
formate-tetrahydrofolate ligase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005448`	molecular activity
methenyltetrahydrofolate cyclohydrolase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005468`	molecular activity
methylenetetrahydrofolate dehydrogenase (NADP+) activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005484`	molecular activity
glycine hydroxymethyltransferase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005502`	molecular activity
methylenetetrahydrofolate reductase [NAD(P)H] activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005519`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
a 5,10-methylenetetrahydrofolate + NAD⁺ -> a 5,10-methenyltetrahydrofolate + NADH `gomodel:1.5.1.15-RXN`	has_output Corrected the methenyl endpoint; the distinct methylene input is retained.	(6R)-5,10-methenyltetrahydrofolate `CHEBI:57455`	UniProtKB:Q02046 Source assertion: Q02046 RHEA:22892 distinguishes the methenyl folate CHEBI:57455 from the methylene folate; the native GO-CAM CHEBI:20502 endpoint incorrectly merged these oxidation states. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_catalytic_activity%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:9600fe7f1e55a4f51554b244607a706b1604904dc71de8960b2f399bf75b0c7c] #/results/2405/comments/1`
glycine + a tetrahydrofolate + NAD⁺ -> a 5,10-methylenetetrahydrofolate + ammonium + CO₂ + NADH `gomodel:GCVMULTI-RXN`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GCVMULTI-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/1; endpoint types: /individuals/32/type; /individuals/91/type`
a tetrahydrofolate + NADP⁺ <- a 7,8-dihydrofolate + NADPH + H⁺ `gomodel:DIHYDROFOLATEREDUCT-RXN`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:DIHYDROFOLATEREDUCT-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/3; endpoint types: /individuals/30/type; /individuals/91/type`
glycine + a tetrahydrofolate + NAD⁺ -> a 5,10-methylenetetrahydrofolate + ammonium + CO₂ + NADH `gomodel:GCVMULTI-RXN`	occurs_in	mitochondrion `GO:0005739`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GCVMULTI-RXN BFO:0000066 gomodel:YeastPathways_PWY3O-697/66a3e0bb00001309. Source location `./YeastPathways_PWY3O-697.json#/facts/4; endpoint types: /individuals/32/type; /individuals/203/type` SGD:S000218158 Source assertion: Complex Portal CPX-1268 places this complex's catalytic function in the mitochondrion. Source location `CPX-1268.json#/functions/0`
methylenetetrahydrofolate reductase [NAD(P)H] activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005519`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005519 RO:0002233 gomodel:YeastPathways_PWY3O-697/6a4c244800005520. Source location `./YeastPathways_PWY3O-697.json#/facts/5; endpoint types: /individuals/171/type; /individuals/172/type`
glycine hydroxymethyltransferase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005502`	provides_input_for	methylenetetrahydrofolate reductase [NAD(P)H] activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005519`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005502 RO:0002413 gomodel:YeastPathways_PWY3O-697/6a4c244800005519. Source location `./YeastPathways_PWY3O-697.json#/facts/6; endpoint types: /individuals/155/type; /individuals/171/type`
a tetrahydrofolate + NADP⁺ <- a 7,8-dihydrofolate + NADPH + H⁺ `gomodel:DIHYDROFOLATEREDUCT-RXN`	has_output	tetrahydrofolate `CHEBI:67016`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:DIHYDROFOLATEREDUCT-RXN RO:0002234 gomodel:YeastPathways_PWY3O-697/66b5638000000268. Source location `./YeastPathways_PWY3O-697.json#/facts/8; endpoint types: /individuals/30/type; /individuals/205/type`
methylenetetrahydrofolate reductase [NAD(P)H] activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005519`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005519 BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/10; endpoint types: /individuals/171/type; /individuals/91/type`
a 5,10-methylenetetrahydrofolate + dUMP -> dTMP + a 7,8-dihydrofolate `gomodel:THYMIDYLATESYN-RXN`	has_output	dTMP(2-) `CHEBI:63528`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:THYMIDYLATESYN-RXN RO:0002234 gomodel:CHEBI_63528_THYMIDYLATESYN-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/11; endpoint types: /individuals/36/type; /individuals/29/type`
glycine + a tetrahydrofolate + NAD⁺ -> a 5,10-methylenetetrahydrofolate + ammonium + CO₂ + NADH `gomodel:GCVMULTI-RXN`	has_input	glycine zwitterion `CHEBI:57305`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GCVMULTI-RXN RO:0002233 gomodel:CHEBI_57305_GCVMULTI-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/12; endpoint types: /individuals/32/type; /individuals/16/type`
a tetrahydrofolate + ATP + formate <=> a 10-formyltetrahydrofolate + ADP + phosphate `gomodel:FORMATETHFLIG-RXN`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:FORMATETHFLIG-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/13; endpoint types: /individuals/31/type; /individuals/91/type`
a 5-methyltetrahydrofolate + NAD⁺ <- a 5,10-methylenetetrahydrofolate + NADH + H⁺ `gomodel:1.5.1.20-RXN`	has_output	NAD(1-) `CHEBI:57540`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.20-RXN RO:0002234 gomodel:CHEBI_57540_1.5.1.20-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/16; endpoint types: /individuals/90/type; /individuals/20/type`
a tetrahydrofolate + ATP + formate <=> a 10-formyltetrahydrofolate + ADP + phosphate `gomodel:FORMATETHFLIG-RXN`	has_output	tetrahydrofolate `CHEBI:67016`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:FORMATETHFLIG-RXN RO:0002234 gomodel:YeastPathways_PWY3O-697/66b5638000000275. Source location `./YeastPathways_PWY3O-697.json#/facts/17; endpoint types: /individuals/31/type; /individuals/210/type`
a 5-methyltetrahydrofolate + NAD⁺ <- a 5,10-methylenetetrahydrofolate + NADH + H⁺ `gomodel:1.5.1.20-RXN`	has_output	5-methyltetrahydrofolate `CHEBI:20612`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.20-RXN RO:0002234 gomodel:YeastPathways_PWY3O-697/66a3e0bb00001215. Source location `./YeastPathways_PWY3O-697.json#/facts/18; endpoint types: /individuals/90/type; /individuals/191/type`
methenyltetrahydrofolate cyclohydrolase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005468`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005468 BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/19; endpoint types: /individuals/123/type; /individuals/91/type`
methenyltetrahydrofolate cyclohydrolase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005468`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005468 RO:0002234 gomodel:YeastPathways_PWY3O-697/6a4c244800005470. Source location `./YeastPathways_PWY3O-697.json#/facts/22; endpoint types: /individuals/123/type; /individuals/125/type`
glycine hydroxymethyltransferase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005502`	has_input	tetrahydrofolate `CHEBI:67016`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005502 RO:0002233 gomodel:YeastPathways_PWY3O-697/6a4c244800005504. Source location `./YeastPathways_PWY3O-697.json#/facts/23; endpoint types: /individuals/155/type; /individuals/157/type`
formate-tetrahydrofolate ligase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005448`	has_output	formate `CHEBI:15740`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005448 RO:0002234 gomodel:YeastPathways_PWY3O-697/6a4c244800005452. Source location `./YeastPathways_PWY3O-697.json#/facts/24; endpoint types: /individuals/104/type; /individuals/108/type`
a 5,10-methylenetetrahydrofolate + NAD⁺ -> a 5,10-methenyltetrahydrofolate + NADH `gomodel:1.5.1.15-RXN`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.15-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/25; endpoint types: /individuals/89/type; /individuals/91/type`
a 5,10-methenyltetrahydrofolate + H₂O <=> a 10-formyltetrahydrofolate + H⁺ `gomodel:METHENYLTHFCYCLOHYDRO-RXN`	has_output	10-formyltetrahydrofolyl-(Glu)n polyanion `CHEBI:134413`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:METHENYLTHFCYCLOHYDRO-RXN RO:0002234 gomodel:CHEBI_134413_METHENYLTHFCYCLOHYDRO-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/26; endpoint types: /individuals/34/type; /individuals/0/type`
formate-tetrahydrofolate ligase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005448`	occurs_in	cytoplasm `GO:0005737`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005448 BFO:0000066 gomodel:YeastPathways_PWY3O-697/6a4c244800005456. Source location `./YeastPathways_PWY3O-697.json#/facts/27; endpoint types: /individuals/104/type; /individuals/111/type`
L-serine + a tetrahydrofolate <=> glycine + a 5,10-methylenetetrahydrofolate + H₂O `gomodel:GLYOHMETRANS-RXN`	has_input	L-serine zwitterion `CHEBI:33384`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GLYOHMETRANS-RXN RO:0002233 gomodel:CHEBI_33384_GLYOHMETRANS-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/28; endpoint types: /individuals/33/type; /individuals/13/type`
a 5-methyltetrahydrofolate + NAD⁺ <- a 5,10-methylenetetrahydrofolate + NADH + H⁺ `gomodel:1.5.1.20-RXN`	has_input	5,10-methylenetetrahydrofolic acid `CHEBI:20502`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.20-RXN RO:0002233 gomodel:CHEBI_20502_GLYOHMETRANS-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/30; endpoint types: /individuals/90/type; /individuals/9/type`
MIS1 Scer `SGD:S000000288`	enables	formate-tetrahydrofolate ligase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005448`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005448 RO:0002333 gomodel:YeastPathways_PWY3O-697/6a4c244800005455. Source location `./YeastPathways_PWY3O-697.json#/facts/32; endpoint types: /individuals/104/type; /individuals/110/type`
L-serine + a tetrahydrofolate <=> glycine + a 5,10-methylenetetrahydrofolate + H₂O `gomodel:GLYOHMETRANS-RXN`	has_input	tetrahydrofolate `CHEBI:67016`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GLYOHMETRANS-RXN RO:0002233 gomodel:YeastPathways_PWY3O-697/6a4c244800005435. Source location `./YeastPathways_PWY3O-697.json#/facts/33; endpoint types: /individuals/33/type; /individuals/92/type`
ADE3 Scer `SGD:S000003436`	enables	a tetrahydrofolate + ATP + formate <=> a 10-formyltetrahydrofolate + ADP + phosphate `gomodel:FORMATETHFLIG-RXN`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:FORMATETHFLIG-RXN RO:0002333 gomodel:YGR204W-MONOMER_FORMATETHFLIG-RXN_controller. Source location `./YeastPathways_PWY3O-697.json#/facts/37; endpoint types: /individuals/31/type; /individuals/37/type`
a 5,10-methylenetetrahydrofolate + NADP⁺ <=> a 5,10-methenyltetrahydrofolate + NADPH `gomodel:METHYLENETHFDEHYDROG-NADP-RXN`	has_input	5,10-methylenetetrahydrofolic acid `CHEBI:20502`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:METHYLENETHFDEHYDROG-NADP-RXN RO:0002233 gomodel:CHEBI_20502_GLYOHMETRANS-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/38; endpoint types: /individuals/35/type; /individuals/9/type`
methylenetetrahydrofolate reductase [NAD(P)H] activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005519`	has_output	5-methyltetrahydrofolate `CHEBI:20612`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005519 RO:0002234 gomodel:YeastPathways_PWY3O-697/6a4c244800005523. Source location `./YeastPathways_PWY3O-697.json#/facts/39; endpoint types: /individuals/171/type; /individuals/175/type`
a 5,10-methylenetetrahydrofolate + dUMP -> dTMP + a 7,8-dihydrofolate `gomodel:THYMIDYLATESYN-RXN`	provides_input_for	a tetrahydrofolate + NADP⁺ <- a 7,8-dihydrofolate + NADPH + H⁺ `gomodel:DIHYDROFOLATEREDUCT-RXN`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:THYMIDYLATESYN-RXN RO:0002413 gomodel:DIHYDROFOLATEREDUCT-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/40; endpoint types: /individuals/36/type; /individuals/30/type`
a tetrahydrofolate + ATP + formate <=> a 10-formyltetrahydrofolate + ADP + phosphate `gomodel:FORMATETHFLIG-RXN`	has_input	10-formyltetrahydrofolyl-(Glu)n polyanion `CHEBI:134413`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:FORMATETHFLIG-RXN RO:0002233 gomodel:CHEBI_134413_METHENYLTHFCYCLOHYDRO-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/41; endpoint types: /individuals/31/type; /individuals/0/type`
L-serine + a tetrahydrofolate <=> glycine + a 5,10-methylenetetrahydrofolate + H₂O `gomodel:GLYOHMETRANS-RXN`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GLYOHMETRANS-RXN RO:0002234 gomodel:CHEBI_15377_GLYOHMETRANS-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/42; endpoint types: /individuals/33/type; /individuals/1/type`
MTD1 Scer `SGD:S000001788`	enables	a 5,10-methylenetetrahydrofolate + NAD⁺ -> a 5,10-methenyltetrahydrofolate + NADH `gomodel:1.5.1.15-RXN`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.15-RXN RO:0002333 gomodel:YeastPathways_PWY3O-697/66a3e0bb00001221. Source location `./YeastPathways_PWY3O-697.json#/facts/44; endpoint types: /individuals/89/type; /individuals/197/type`
a tetrahydrofolate + ATP + formate <=> a 10-formyltetrahydrofolate + ADP + phosphate `gomodel:FORMATETHFLIG-RXN`	occurs_in	cytoplasm `GO:0005737`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:FORMATETHFLIG-RXN BFO:0000066 gomodel:YeastPathways_PWY3O-697/66b5638000000273. Source location `./YeastPathways_PWY3O-697.json#/facts/45; endpoint types: /individuals/31/type; /individuals/208/type`
a tetrahydrofolate + ATP + formate <=> a 10-formyltetrahydrofolate + ADP + phosphate `gomodel:FORMATETHFLIG-RXN`	has_input	hydrogenphosphate `CHEBI:43474`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:FORMATETHFLIG-RXN RO:0002233 gomodel:CHEBI_43474_FORMATETHFLIG-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/46; endpoint types: /individuals/31/type; /individuals/14/type`
methylenetetrahydrofolate reductase [NAD(P)H] activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005519`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005519 BFO:0000066 gomodel:YeastPathways_PWY3O-697/6a4c244800005526. Source location `./YeastPathways_PWY3O-697.json#/facts/48; endpoint types: /individuals/171/type; /individuals/177/type`
CPX-1268 Scer `SGD:S000218158`	enables	glycine + a tetrahydrofolate + NAD⁺ -> a 5,10-methylenetetrahydrofolate + ammonium + CO₂ + NADH `gomodel:GCVMULTI-RXN`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GCVMULTI-RXN RO:0002333 gomodel:YeastPathways_PWY3O-697/66a3e0bb00001307. Source location `./YeastPathways_PWY3O-697.json#/facts/49; endpoint types: /individuals/32/type; /individuals/201/type`
a 5,10-methenyltetrahydrofolate + H₂O <=> a 10-formyltetrahydrofolate + H⁺ `gomodel:METHENYLTHFCYCLOHYDRO-RXN`	has_input Corrected the methenyl endpoint; the distinct methylene input is retained.	(6R)-5,10-methenyltetrahydrofolate `CHEBI:57455`	UniProtKB:P07245 Source assertion: P07245 RHEA:23700 distinguishes the methenyl folate CHEBI:57455 from the methylene folate; the native GO-CAM CHEBI:20502 endpoint incorrectly merged these oxidation states. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_catalytic_activity%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:9600fe7f1e55a4f51554b244607a706b1604904dc71de8960b2f399bf75b0c7c] #/results/160/comments/2`
a 5,10-methylenetetrahydrofolate + NADP⁺ <=> a 5,10-methenyltetrahydrofolate + NADPH `gomodel:METHYLENETHFDEHYDROG-NADP-RXN`	has_output Corrected the methenyl endpoint; the distinct methylene input is retained.	(6R)-5,10-methenyltetrahydrofolate `CHEBI:57455`	UniProtKB:P07245 Source assertion: P07245 RHEA:22812 distinguishes the methenyl folate CHEBI:57455 from the methylene folate; the native GO-CAM CHEBI:20502 endpoint incorrectly merged these oxidation states. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_catalytic_activity%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:9600fe7f1e55a4f51554b244607a706b1604904dc71de8960b2f399bf75b0c7c] #/results/160/comments/1`
MET12 Scer `SGD:S000005944`	enables	a 5-methyltetrahydrofolate + NAD⁺ <- a 5,10-methylenetetrahydrofolate + NADH + H⁺ `gomodel:1.5.1.20-RXN`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.20-RXN RO:0002333 gomodel:YPL023C-MONOMER_1.5.1.20-RXN_controller. Source location `./YeastPathways_PWY3O-697.json#/facts/52; endpoint types: /individuals/90/type; /individuals/41/type`
formate-tetrahydrofolate ligase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005448`	has_output	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005448 RO:0002234 gomodel:YeastPathways_PWY3O-697/6a4c244800005453. Source location `./YeastPathways_PWY3O-697.json#/facts/53; endpoint types: /individuals/104/type; /individuals/109/type`
DFR1 Scer `SGD:S000005762`	enables	a tetrahydrofolate + NADP⁺ <- a 7,8-dihydrofolate + NADPH + H⁺ `gomodel:DIHYDROFOLATEREDUCT-RXN`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:DIHYDROFOLATEREDUCT-RXN RO:0002333 gomodel:YOR236W-MONOMER_DIHYDROFOLATEREDUCT-RXN_controller. Source location `./YeastPathways_PWY3O-697.json#/facts/54; endpoint types: /individuals/30/type; /individuals/40/type`
a 5,10-methylenetetrahydrofolate + NAD⁺ -> a 5,10-methenyltetrahydrofolate + NADH `gomodel:1.5.1.15-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.15-RXN BFO:0000066 gomodel:reaction_1.5.1.15-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY3O-697.json#/facts/55; endpoint types: /individuals/89/type; /individuals/83/type`
a tetrahydrofolate + ATP + formate <=> a 10-formyltetrahydrofolate + ADP + phosphate `gomodel:FORMATETHFLIG-RXN`	has_output	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:FORMATETHFLIG-RXN RO:0002234 gomodel:CHEBI_30616_FORMATETHFLIG-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/56; endpoint types: /individuals/31/type; /individuals/12/type`
glycine hydroxymethyltransferase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005502`	provides_input_for	a 5-methyltetrahydrofolate + NAD⁺ <- a 5,10-methylenetetrahydrofolate + NADH + H⁺ `gomodel:1.5.1.20-RXN`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005502 RO:0002413 gomodel:1.5.1.20-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/57; endpoint types: /individuals/155/type; /individuals/90/type`
MET13 Scer `SGD:S000003093`	enables	methylenetetrahydrofolate reductase [NAD(P)H] activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005519`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005519 RO:0002333 gomodel:YeastPathways_PWY3O-697/6a4c244800005525. Source location `./YeastPathways_PWY3O-697.json#/facts/59; endpoint types: /individuals/171/type; /individuals/176/type`
a tetrahydrofolate + NADP⁺ <- a 7,8-dihydrofolate + NADPH + H⁺ `gomodel:DIHYDROFOLATEREDUCT-RXN`	has_input	dihydrofolate(2-) `CHEBI:57451`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:DIHYDROFOLATEREDUCT-RXN RO:0002233 gomodel:CHEBI_57451_THYMIDYLATESYN-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/60; endpoint types: /individuals/30/type; /individuals/18/type`
a 5-methyltetrahydrofolate + NAD⁺ <- a 5,10-methylenetetrahydrofolate + NADH + H⁺ `gomodel:1.5.1.20-RXN`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.20-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/61; endpoint types: /individuals/90/type; /individuals/91/type`
methylenetetrahydrofolate reductase [NAD(P)H] activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005519`	has_input	5,10-methylenetetrahydrofolic acid `CHEBI:20502`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005519 RO:0002233 gomodel:CHEBI_20502_GLYOHMETRANS-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/62; endpoint types: /individuals/171/type; /individuals/9/type`
a 5-methyltetrahydrofolate + NAD⁺ <- a 5,10-methylenetetrahydrofolate + NADH + H⁺ `gomodel:1.5.1.20-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.20-RXN BFO:0000066 gomodel:reaction_1.5.1.20-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY3O-697.json#/facts/63; endpoint types: /individuals/90/type; /individuals/84/type`
a tetrahydrofolate + NADP⁺ <- a 7,8-dihydrofolate + NADPH + H⁺ `gomodel:DIHYDROFOLATEREDUCT-RXN`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:DIHYDROFOLATEREDUCT-RXN RO:0002234 gomodel:CHEBI_58349_DIHYDROFOLATEREDUCT-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/65; endpoint types: /individuals/30/type; /individuals/27/type`
a 5,10-methylenetetrahydrofolate + NAD⁺ -> a 5,10-methenyltetrahydrofolate + NADH `gomodel:1.5.1.15-RXN`	has_input	5,10-methylenetetrahydrofolic acid `CHEBI:20502`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.15-RXN RO:0002233 gomodel:CHEBI_20502_GLYOHMETRANS-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/66; endpoint types: /individuals/89/type; /individuals/9/type`
SHM2 Scer `SGD:S000004048`	enables	glycine hydroxymethyltransferase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005502`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005502 RO:0002333 gomodel:YeastPathways_PWY3O-697/6a4c244800005507. Source location `./YeastPathways_PWY3O-697.json#/facts/67; endpoint types: /individuals/155/type; /individuals/160/type`
a 5,10-methylenetetrahydrofolate + dUMP -> dTMP + a 7,8-dihydrofolate `gomodel:THYMIDYLATESYN-RXN`	has_input	5,10-methylenetetrahydrofolic acid `CHEBI:20502`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:THYMIDYLATESYN-RXN RO:0002233 gomodel:CHEBI_20502_GCVMULTI-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/68; endpoint types: /individuals/36/type; /individuals/8/type`
glycine + a tetrahydrofolate + NAD⁺ -> a 5,10-methylenetetrahydrofolate + ammonium + CO₂ + NADH `gomodel:GCVMULTI-RXN`	has_output	ammonium `CHEBI:28938`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GCVMULTI-RXN RO:0002234 gomodel:CHEBI_28938_GCVMULTI-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/69; endpoint types: /individuals/32/type; /individuals/11/type`
glycine hydroxymethyltransferase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005502`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005502 BFO:0000066 gomodel:YeastPathways_PWY3O-697/6a4c244800005509. Source location `./YeastPathways_PWY3O-697.json#/facts/70; endpoint types: /individuals/155/type; /individuals/161/type`
MIS1 Scer `SGD:S000000288`	enables	methenyltetrahydrofolate cyclohydrolase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005468`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005468 RO:0002333 gomodel:YeastPathways_PWY3O-697/6a4c244800005471. Source location `./YeastPathways_PWY3O-697.json#/facts/71; endpoint types: /individuals/123/type; /individuals/126/type`
a tetrahydrofolate + NADP⁺ <- a 7,8-dihydrofolate + NADPH + H⁺ `gomodel:DIHYDROFOLATEREDUCT-RXN`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:DIHYDROFOLATEREDUCT-RXN RO:0002233 gomodel:CHEBI_57783_DIHYDROFOLATEREDUCT-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/72; endpoint types: /individuals/30/type; /individuals/22/type`
a 5,10-methylenetetrahydrofolate + NAD⁺ -> a 5,10-methenyltetrahydrofolate + NADH `gomodel:1.5.1.15-RXN`	has_output	NADH(2-) `CHEBI:57945`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.15-RXN RO:0002234 gomodel:CHEBI_57945_1.5.1.15-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/73; endpoint types: /individuals/89/type; /individuals/24/type`
a tetrahydrofolate + NADP⁺ <- a 7,8-dihydrofolate + NADPH + H⁺ `gomodel:DIHYDROFOLATEREDUCT-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:DIHYDROFOLATEREDUCT-RXN BFO:0000066 gomodel:reaction_DIHYDROFOLATEREDUCT-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY3O-697.json#/facts/74; endpoint types: /individuals/30/type; /individuals/85/type`
L-serine + a tetrahydrofolate <=> glycine + a 5,10-methylenetetrahydrofolate + H₂O `gomodel:GLYOHMETRANS-RXN`	has_output	5,10-methylenetetrahydrofolic acid `CHEBI:20502`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GLYOHMETRANS-RXN RO:0002234 gomodel:CHEBI_20502_GLYOHMETRANS-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/75; endpoint types: /individuals/33/type; /individuals/9/type`
a 5,10-methylenetetrahydrofolate + dUMP -> dTMP + a 7,8-dihydrofolate `gomodel:THYMIDYLATESYN-RXN`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:THYMIDYLATESYN-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/76; endpoint types: /individuals/36/type; /individuals/91/type`
a 5-methyltetrahydrofolate + NAD⁺ <- a 5,10-methylenetetrahydrofolate + NADH + H⁺ `gomodel:1.5.1.20-RXN`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.20-RXN RO:0002233 gomodel:CHEBI_15378_1.5.1.20-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/77; endpoint types: /individuals/90/type; /individuals/3/type`
methylenetetrahydrofolate reductase [NAD(P)H] activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005519`	has_output MET13 uses NADPH/NADP; the independently supported MET12 NADH branch remains.	NADP(3-) `CHEBI:58349`	UniProtKB:P53128 Source assertion: MET13 methylenetetrahydrofolate reductase uses NADPH/NADP, as annotated by RHEA:19817; its reduction direction consumes NADPH. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_catalytic_activity%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:9600fe7f1e55a4f51554b244607a706b1604904dc71de8960b2f399bf75b0c7c] #/results/3945/comments/0` PMID:11729203 Source assertion: The experimental study identifies the yeast MET13-encoded reductase as NADPH-dependent. Source location `PubMed abstract, MET13 cofactor-specificity statement`
methylenetetrahydrofolate dehydrogenase (NADP+) activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005484`	has_output Corrected the methenyl endpoint; the distinct methylene input is retained.	(6R)-5,10-methenyltetrahydrofolate `CHEBI:57455`	UniProtKB:P09440 Source assertion: P09440 RHEA:22812 distinguishes the methenyl folate CHEBI:57455 from the methylene folate; the native GO-CAM CHEBI:20502 endpoint incorrectly merged these oxidation states. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_catalytic_activity%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:9600fe7f1e55a4f51554b244607a706b1604904dc71de8960b2f399bf75b0c7c] #/results/224/comments/1`
L-serine + a tetrahydrofolate <=> glycine + a 5,10-methylenetetrahydrofolate + H₂O `gomodel:GLYOHMETRANS-RXN`	has_output	glycine zwitterion `CHEBI:57305`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GLYOHMETRANS-RXN RO:0002234 gomodel:CHEBI_57305_GLYOHMETRANS-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/80; endpoint types: /individuals/33/type; /individuals/17/type`
methylenetetrahydrofolate dehydrogenase (NADP+) activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005484`	has_input	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005484 RO:0002233 gomodel:YeastPathways_PWY3O-697/6a4c244800005485. Source location `./YeastPathways_PWY3O-697.json#/facts/81; endpoint types: /individuals/138/type; /individuals/139/type`
glycine + a tetrahydrofolate + NAD⁺ -> a 5,10-methylenetetrahydrofolate + ammonium + CO₂ + NADH `gomodel:GCVMULTI-RXN`	has_input	NAD(1-) `CHEBI:57540`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GCVMULTI-RXN RO:0002233 gomodel:CHEBI_57540_GCVMULTI-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/82; endpoint types: /individuals/32/type; /individuals/21/type`
methenyltetrahydrofolate cyclohydrolase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005468`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005468 RO:0002233 gomodel:YeastPathways_PWY3O-697/6a4c244800005469. Source location `./YeastPathways_PWY3O-697.json#/facts/83; endpoint types: /individuals/123/type; /individuals/124/type`
glycine + a tetrahydrofolate + NAD⁺ -> a 5,10-methylenetetrahydrofolate + ammonium + CO₂ + NADH `gomodel:GCVMULTI-RXN`	has_output	carbon dioxide `CHEBI:16526`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GCVMULTI-RXN RO:0002234 gomodel:CHEBI_16526_GCVMULTI-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/84; endpoint types: /individuals/32/type; /individuals/7/type`
SHM1 Scer `SGD:S000000467`	enables	L-serine + a tetrahydrofolate <=> glycine + a 5,10-methylenetetrahydrofolate + H₂O `gomodel:GLYOHMETRANS-RXN`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GLYOHMETRANS-RXN RO:0002333 gomodel:YeastPathways_PWY3O-697/66b5638000000277. Source location `./YeastPathways_PWY3O-697.json#/facts/85; endpoint types: /individuals/33/type; /individuals/212/type`
formate-tetrahydrofolate ligase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005448`	has_input	ADP(3-) `CHEBI:456216`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005448 RO:0002233 gomodel:YeastPathways_PWY3O-697/6a4c244800005449. Source location `./YeastPathways_PWY3O-697.json#/facts/86; endpoint types: /individuals/104/type; /individuals/105/type`
a 5,10-methylenetetrahydrofolate + dUMP -> dTMP + a 7,8-dihydrofolate `gomodel:THYMIDYLATESYN-RXN`	has_output	dihydrofolate(2-) `CHEBI:57451`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:THYMIDYLATESYN-RXN RO:0002234 gomodel:CHEBI_57451_THYMIDYLATESYN-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/88; endpoint types: /individuals/36/type; /individuals/18/type`
methenyltetrahydrofolate cyclohydrolase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005468`	has_input Corrected the methenyl endpoint; the distinct methylene input is retained.	(6R)-5,10-methenyltetrahydrofolate `CHEBI:57455`	UniProtKB:P09440 Source assertion: P09440 RHEA:23700 distinguishes the methenyl folate CHEBI:57455 from the methylene folate; the native GO-CAM CHEBI:20502 endpoint incorrectly merged these oxidation states. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_catalytic_activity%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:9600fe7f1e55a4f51554b244607a706b1604904dc71de8960b2f399bf75b0c7c] #/results/224/comments/2`
formate-tetrahydrofolate ligase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005448`	has_input	hydrogenphosphate `CHEBI:43474`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005448 RO:0002233 gomodel:YeastPathways_PWY3O-697/6a4c244800005450. Source location `./YeastPathways_PWY3O-697.json#/facts/90; endpoint types: /individuals/104/type; /individuals/106/type`
a 5,10-methylenetetrahydrofolate + NADP⁺ <=> a 5,10-methenyltetrahydrofolate + NADPH `gomodel:METHYLENETHFDEHYDROG-NADP-RXN`	has_input	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:METHYLENETHFDEHYDROG-NADP-RXN RO:0002233 gomodel:CHEBI_58349_METHYLENETHFDEHYDROG-NADP-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/91; endpoint types: /individuals/35/type; /individuals/28/type`
a 5,10-methylenetetrahydrofolate + NADP⁺ <=> a 5,10-methenyltetrahydrofolate + NADPH `gomodel:METHYLENETHFDEHYDROG-NADP-RXN`	has_output	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:METHYLENETHFDEHYDROG-NADP-RXN RO:0002234 gomodel:CHEBI_57783_METHYLENETHFDEHYDROG-NADP-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/92; endpoint types: /individuals/35/type; /individuals/23/type`
a tetrahydrofolate + ATP + formate <=> a 10-formyltetrahydrofolate + ADP + phosphate `gomodel:FORMATETHFLIG-RXN`	has_input	ADP(3-) `CHEBI:456216`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:FORMATETHFLIG-RXN RO:0002233 gomodel:CHEBI_456216_FORMATETHFLIG-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/93; endpoint types: /individuals/31/type; /individuals/15/type`
a 5,10-methenyltetrahydrofolate + H₂O <=> a 10-formyltetrahydrofolate + H⁺ `gomodel:METHENYLTHFCYCLOHYDRO-RXN`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:METHENYLTHFCYCLOHYDRO-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/94; endpoint types: /individuals/34/type; /individuals/91/type`
glycine + a tetrahydrofolate + NAD⁺ -> a 5,10-methylenetetrahydrofolate + ammonium + CO₂ + NADH `gomodel:GCVMULTI-RXN`	has_output	NADH(2-) `CHEBI:57945`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GCVMULTI-RXN RO:0002234 gomodel:CHEBI_57945_GCVMULTI-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/95; endpoint types: /individuals/32/type; /individuals/26/type`
a 5,10-methylenetetrahydrofolate + NAD⁺ -> a 5,10-methenyltetrahydrofolate + NADH `gomodel:1.5.1.15-RXN`	has_input	NAD(1-) `CHEBI:57540`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.15-RXN RO:0002233 gomodel:CHEBI_57540_1.5.1.15-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/96; endpoint types: /individuals/89/type; /individuals/19/type`
glycine hydroxymethyltransferase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005502`	has_output	glycine zwitterion `CHEBI:57305`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005502 RO:0002234 gomodel:YeastPathways_PWY3O-697/6a4c244800005506. Source location `./YeastPathways_PWY3O-697.json#/facts/97; endpoint types: /individuals/155/type; /individuals/159/type`
a 5-methyltetrahydrofolate + NAD⁺ <- a 5,10-methylenetetrahydrofolate + NADH + H⁺ `gomodel:1.5.1.20-RXN`	has_input	NADH(2-) `CHEBI:57945`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:1.5.1.20-RXN RO:0002233 gomodel:CHEBI_57945_1.5.1.20-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/98; endpoint types: /individuals/90/type; /individuals/25/type`
methenyltetrahydrofolate cyclohydrolase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005468`	has_output	10-formyltetrahydrofolyl-(Glu)n polyanion `CHEBI:134413`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005468 RO:0002234 gomodel:CHEBI_134413_METHENYLTHFCYCLOHYDRO-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/99; endpoint types: /individuals/123/type; /individuals/0/type`
methylenetetrahydrofolate dehydrogenase (NADP+) activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005484`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005484 BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/100; endpoint types: /individuals/138/type; /individuals/91/type`
a 5,10-methylenetetrahydrofolate + NADP⁺ <=> a 5,10-methenyltetrahydrofolate + NADPH `gomodel:METHYLENETHFDEHYDROG-NADP-RXN`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:METHYLENETHFDEHYDROG-NADP-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/101; endpoint types: /individuals/35/type; /individuals/91/type`
glycine + a tetrahydrofolate + NAD⁺ -> a 5,10-methylenetetrahydrofolate + ammonium + CO₂ + NADH `gomodel:GCVMULTI-RXN`	has_input	tetrahydrofolate `CHEBI:67016`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GCVMULTI-RXN RO:0002233 gomodel:YeastPathways_PWY3O-697/6a4c244800005437. Source location `./YeastPathways_PWY3O-697.json#/facts/102; endpoint types: /individuals/32/type; /individuals/94/type`
glycine + a tetrahydrofolate + NAD⁺ -> a 5,10-methylenetetrahydrofolate + ammonium + CO₂ + NADH `gomodel:GCVMULTI-RXN`	has_output	5,10-methylenetetrahydrofolic acid `CHEBI:20502`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GCVMULTI-RXN RO:0002234 gomodel:CHEBI_20502_GLYOHMETRANS-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/103; endpoint types: /individuals/32/type; /individuals/9/type`
methylenetetrahydrofolate reductase [NAD(P)H] activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005519`	has_input MET13 uses NADPH/NADP; the independently supported MET12 NADH branch remains.	NADPH(4-) `CHEBI:57783`	UniProtKB:P53128 Source assertion: MET13 methylenetetrahydrofolate reductase uses NADPH/NADP, as annotated by RHEA:19817; its reduction direction consumes NADPH. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_catalytic_activity%2Ccc_function%2Ccc_caution%2Corganism_id [sha256:9600fe7f1e55a4f51554b244607a706b1604904dc71de8960b2f399bf75b0c7c] #/results/3945/comments/0` PMID:11729203 Source assertion: The experimental study identifies the yeast MET13-encoded reductase as NADPH-dependent. Source location `PubMed abstract, MET13 cofactor-specificity statement`
CDC21 Scer `SGD:S000005600`	enables	a 5,10-methylenetetrahydrofolate + dUMP -> dTMP + a 7,8-dihydrofolate `gomodel:THYMIDYLATESYN-RXN`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:THYMIDYLATESYN-RXN RO:0002333 gomodel:YeastPathways_PWY3O-697/66a3e0bb00001212. Source location `./YeastPathways_PWY3O-697.json#/facts/105; endpoint types: /individuals/36/type; /individuals/189/type`
methylenetetrahydrofolate dehydrogenase (NADP+) activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005484`	has_output	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005484 RO:0002234 gomodel:YeastPathways_PWY3O-697/6a4c244800005486. Source location `./YeastPathways_PWY3O-697.json#/facts/106; endpoint types: /individuals/138/type; /individuals/140/type`
glycine hydroxymethyltransferase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005502`	has_output	5,10-methylenetetrahydrofolic acid `CHEBI:20502`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005502 RO:0002234 gomodel:CHEBI_20502_GLYOHMETRANS-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/107; endpoint types: /individuals/155/type; /individuals/9/type`
glycine hydroxymethyltransferase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005502`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005502 RO:0002234 gomodel:YeastPathways_PWY3O-697/6a4c244800005505. Source location `./YeastPathways_PWY3O-697.json#/facts/109; endpoint types: /individuals/155/type; /individuals/158/type`
glycine hydroxymethyltransferase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005502`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005502 BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/110; endpoint types: /individuals/155/type; /individuals/91/type`
a 5,10-methenyltetrahydrofolate + H₂O <=> a 10-formyltetrahydrofolate + H⁺ `gomodel:METHENYLTHFCYCLOHYDRO-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:METHENYLTHFCYCLOHYDRO-RXN RO:0002234 gomodel:CHEBI_15378_METHENYLTHFCYCLOHYDRO-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/111; endpoint types: /individuals/34/type; /individuals/5/type`
ADE3 Scer `SGD:S000003436`	enables	a 5,10-methylenetetrahydrofolate + NADP⁺ <=> a 5,10-methenyltetrahydrofolate + NADPH `gomodel:METHYLENETHFDEHYDROG-NADP-RXN`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:METHYLENETHFDEHYDROG-NADP-RXN RO:0002333 gomodel:YGR204W-MONOMER_METHYLENETHFDEHYDROG-NADP-RXN_controller. Source location `./YeastPathways_PWY3O-697.json#/facts/112; endpoint types: /individuals/35/type; /individuals/39/type`
methylenetetrahydrofolate dehydrogenase (NADP+) activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005484`	has_input	5,10-methylenetetrahydrofolic acid `CHEBI:20502`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005484 RO:0002233 gomodel:CHEBI_20502_GLYOHMETRANS-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/113; endpoint types: /individuals/138/type; /individuals/9/type`
L-serine + a tetrahydrofolate <=> glycine + a 5,10-methylenetetrahydrofolate + H₂O `gomodel:GLYOHMETRANS-RXN`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:GLYOHMETRANS-RXN BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/115; endpoint types: /individuals/33/type; /individuals/91/type`
methenyltetrahydrofolate cyclohydrolase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005468`	occurs_in	cytoplasm `GO:0005737`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005468 BFO:0000066 gomodel:YeastPathways_PWY3O-697/6a4c244800005473. Source location `./YeastPathways_PWY3O-697.json#/facts/116; endpoint types: /individuals/123/type; /individuals/127/type`
formate-tetrahydrofolate ligase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005448`	part_of	folic acid metabolic process `GO:0046655`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005448 BFO:0000050 gomodel:YeastPathways_PWY3O-697/YeastPathways_PWY3O-697. Source location `./YeastPathways_PWY3O-697.json#/facts/117; endpoint types: /individuals/104/type; /individuals/91/type`
a 5,10-methenyltetrahydrofolate + H₂O <=> a 10-formyltetrahydrofolate + H⁺ `gomodel:METHENYLTHFCYCLOHYDRO-RXN`	occurs_in	cytoplasm `GO:0005737`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:METHENYLTHFCYCLOHYDRO-RXN BFO:0000066 gomodel:YeastPathways_PWY3O-697/66b5638000000281. Source location `./YeastPathways_PWY3O-697.json#/facts/118; endpoint types: /individuals/34/type; /individuals/214/type`
MIS1 Scer `SGD:S000000288`	enables	methylenetetrahydrofolate dehydrogenase (NADP+) activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005484`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005484 RO:0002333 gomodel:YeastPathways_PWY3O-697/6a4c244800005487. Source location `./YeastPathways_PWY3O-697.json#/facts/119; endpoint types: /individuals/138/type; /individuals/141/type`
a tetrahydrofolate + ATP + formate <=> a 10-formyltetrahydrofolate + ADP + phosphate `gomodel:FORMATETHFLIG-RXN`	has_output	formate `CHEBI:15740`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:FORMATETHFLIG-RXN RO:0002234 gomodel:CHEBI_15740_FORMATETHFLIG-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/120; endpoint types: /individuals/31/type; /individuals/6/type`
formate-tetrahydrofolate ligase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005448`	has_output	tetrahydrofolate `CHEBI:67016`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005448 RO:0002234 gomodel:YeastPathways_PWY3O-697/6a4c244800005451. Source location `./YeastPathways_PWY3O-697.json#/facts/121; endpoint types: /individuals/104/type; /individuals/107/type`
a 5,10-methenyltetrahydrofolate + H₂O <=> a 10-formyltetrahydrofolate + H⁺ `gomodel:METHENYLTHFCYCLOHYDRO-RXN`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:METHENYLTHFCYCLOHYDRO-RXN RO:0002233 gomodel:CHEBI_15377_METHENYLTHFCYCLOHYDRO-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/122; endpoint types: /individuals/34/type; /individuals/2/type`
glycine hydroxymethyltransferase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005502`	has_input	L-serine zwitterion `CHEBI:33384`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005502 RO:0002233 gomodel:YeastPathways_PWY3O-697/6a4c244800005503. Source location `./YeastPathways_PWY3O-697.json#/facts/123; endpoint types: /individuals/155/type; /individuals/156/type`
formate-tetrahydrofolate ligase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005448`	has_input	10-formyltetrahydrofolyl-(Glu)n polyanion `CHEBI:134413`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:YeastPathways_PWY3O-697/6a4c244800005448 RO:0002233 gomodel:CHEBI_134413_METHENYLTHFCYCLOHYDRO-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/125; endpoint types: /individuals/104/type; /individuals/0/type`
a tetrahydrofolate + NADP⁺ <- a 7,8-dihydrofolate + NADPH + H⁺ `gomodel:DIHYDROFOLATEREDUCT-RXN`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:DIHYDROFOLATEREDUCT-RXN RO:0002233 gomodel:CHEBI_15378_DIHYDROFOLATEREDUCT-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/126; endpoint types: /individuals/30/type; /individuals/4/type`
ADE3 Scer `SGD:S000003436`	enables	a 5,10-methenyltetrahydrofolate + H₂O <=> a 10-formyltetrahydrofolate + H⁺ `gomodel:METHENYLTHFCYCLOHYDRO-RXN`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:METHENYLTHFCYCLOHYDRO-RXN RO:0002333 gomodel:YGR204W-MONOMER_METHENYLTHFCYCLOHYDRO-RXN_controller. Source location `./YeastPathways_PWY3O-697.json#/facts/127; endpoint types: /individuals/34/type; /individuals/38/type`
a 5,10-methylenetetrahydrofolate + NADP⁺ <=> a 5,10-methenyltetrahydrofolate + NADPH `gomodel:METHYLENETHFDEHYDROG-NADP-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:METHYLENETHFDEHYDROG-NADP-RXN BFO:0000066 gomodel:reaction_METHYLENETHFDEHYDROG-NADP-RXN_location_lociGO_0005829. Source location `./YeastPathways_PWY3O-697.json#/facts/128; endpoint types: /individuals/35/type; /individuals/87/type`
a 5,10-methylenetetrahydrofolate + dUMP -> dTMP + a 7,8-dihydrofolate `gomodel:THYMIDYLATESYN-RXN`	has_input	dUMP(2-) `CHEBI:246422`	gomodel:YeastPathways_PWY3O-697 Source assertion: The model asserts gomodel:THYMIDYLATESYN-RXN RO:0002233 gomodel:CHEBI_246422_THYMIDYLATESYN-RXN. Source location `./YeastPathways_PWY3O-697.json#/facts/130; endpoint types: /individuals/36/type; /individuals/10/type`
CPX-1268 Scer `SGD:S000218158`	has_part ECO:0005547: inferred by curator; source stoichiometry minValue: 1, maxValue: 1	LPD1 Scer `SGD:S000001876`	SGD:S000218158 Source assertion: Complex Portal CPX-1268 lists P09624 as a protein component; complex evidence is ECO:0005547: inferred by curator. Source location `Complex Portal JSON #/participants/0; /evidenceType`
CPX-1268 Scer `SGD:S000218158`	has_part ECO:0005547: inferred by curator; stoichiometry unspecified	Glycine cleavage system H protein, mitochondrial `UniProtKB:P39726`	SGD:S000218158 Source assertion: Complex Portal CPX-1268 lists P39726 as a protein component; complex evidence is ECO:0005547: inferred by curator. Source location `Complex Portal JSON #/participants/1; /evidenceType`
CPX-1268 Scer `SGD:S000218158`	has_part ECO:0005547: inferred by curator; stoichiometry unspecified	GCV2 Scer `SGD:S000004801`	SGD:S000218158 Source assertion: Complex Portal CPX-1268 lists P49095 as a protein component; complex evidence is ECO:0005547: inferred by curator. Source location `Complex Portal JSON #/participants/2; /evidenceType`
CPX-1268 Scer `SGD:S000218158`	has_part ECO:0005547: inferred by curator; stoichiometry unspecified	GCV1 Scer `SGD:S000002426`	SGD:S000218158 Source assertion: Complex Portal CPX-1268 lists P48015 as a protein component; complex evidence is ECO:0005547: inferred by curator. Source location `Complex Portal JSON #/participants/3; /evidenceType`
CPX-1268 Scer `SGD:S000218158`	has_part ECO:0005547: inferred by curator; source stoichiometry minValue: 1, maxValue: 1	FAD(3-) `CHEBI:57692`	SGD:S000218158 Source assertion: Complex Portal CPX-1268 lists CHEBI:57692 as a small molecule component; complex evidence is ECO:0005547: inferred by curator. Source location `Complex Portal JSON #/participants/4; /evidenceType`
CPX-1268 Scer `SGD:S000218158`	located_in Complex location from the independent composition source; original GO-CAM activity locations are retained with their own provenance.	mitochondrion `GO:0005739`	SGD:S000218158 Source assertion: Complex Portal CPX-1268 records cellular location GO:0005739 (mitochondrion); its evidence classification is ECO:0005547: inferred by curator. Source location `Complex Portal JSON #/crossReferences/6; /evidenceType`
MIS1 Scer `SGD:S000000288`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:14576278); ECO:0000269 (PubMed:3528153)	mitochondrion `GO:0005739`	UniProtKB:P09440 Source assertion: UniProtKB:P09440 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/224/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P09440 Source assertion: UniProtKB:P09440 has the exact SGD cross-reference SGD:S000000288. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/224/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ADE3 Scer `SGD:S000003436`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:P07245 Source assertion: UniProtKB:P07245 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/160/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P07245 Source assertion: UniProtKB:P07245 has the exact SGD cross-reference SGD:S000003436. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/160/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ADE3 Scer `SGD:S000003436`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	nucleus `GO:0005634`	UniProtKB:P07245 Source assertion: UniProtKB:P07245 annotates location Nucleus (SL-0191). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/160/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P07245 Source assertion: UniProtKB:P07245 has the exact SGD cross-reference SGD:S000003436. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/160/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
MTD1 Scer `SGD:S000001788`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:Q02046 Source assertion: UniProtKB:Q02046 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2405/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q02046 Source assertion: UniProtKB:Q02046 has the exact SGD cross-reference SGD:S000001788. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2405/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
MTD1 Scer `SGD:S000001788`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	nucleus `GO:0005634`	UniProtKB:Q02046 Source assertion: UniProtKB:Q02046 annotates location Nucleus (SL-0191). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2405/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q02046 Source assertion: UniProtKB:Q02046 has the exact SGD cross-reference SGD:S000001788. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2405/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
SHM2 Scer `SGD:S000004048`	located_in Physical protein location from reviewed UniProt. ECO:0000305 (PubMed:8132653)	cytoplasm `GO:0005737`	UniProtKB:P37291 Source assertion: UniProtKB:P37291 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3551/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P37291 Source assertion: UniProtKB:P37291 has the exact SGD cross-reference SGD:S000004048. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3551/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
SHM1 Scer `SGD:S000000467`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	mitochondrion `GO:0005739`	UniProtKB:P37292 Source assertion: UniProtKB:P37292 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1253/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P37292 Source assertion: UniProtKB:P37292 has the exact SGD cross-reference SGD:S000000467. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1253/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
CDC21 Scer `SGD:S000005600`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:8132557) Source qualification: Localizes to the nuclear periphery	nucleus `GO:0005634`	UniProtKB:P06785 Source assertion: UniProtKB:P06785 annotates location Nucleus (SL-0191). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/148/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P06785 Source assertion: UniProtKB:P06785 has the exact SGD cross-reference SGD:S000005600. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/148/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
Glycine cleavage system H protein, mitochondrial `UniProtKB:P39726`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:14576278)	mitochondrion `GO:0005739`	UniProtKB:P39726 Source assertion: UniProtKB:P39726 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1589/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
LPD1 Scer `SGD:S000001876`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	mitochondrial matrix `GO:0005759`	UniProtKB:P09624 Source assertion: UniProtKB:P09624 annotates location Mitochondrion matrix (SL-0170). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/227/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P09624 Source assertion: UniProtKB:P09624 has the exact SGD cross-reference SGD:S000001876. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/227/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
GCV2 Scer `SGD:S000004801`	located_in Physical protein location from reviewed UniProt. ECO:0000250	mitochondrion `GO:0005739`	UniProtKB:P49095 Source assertion: UniProtKB:P49095 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2023/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P49095 Source assertion: UniProtKB:P49095 has the exact SGD cross-reference SGD:S000004801. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2023/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
GCV1 Scer `SGD:S000002426`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	mitochondrion `GO:0005739`	UniProtKB:P48015 Source assertion: UniProtKB:P48015 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3893/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P48015 Source assertion: UniProtKB:P48015 has the exact SGD cross-reference SGD:S000002426. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3893/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
formate-tetrahydrofolate ligase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005448`	occurs_in Activity compartment supported by an inspected functional annotation. ECO:0000269 (PubMed:3528153). Alternative isoenzymes may function elsewhere.	mitochondrion `GO:0005739`	UniProtKB:P09440 Source assertion: UniProtKB:P09440 describes the corresponding catalytic function in the mitochondrion; this projection applies to the activity enabled by SGD:S000000288. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/224/comments/0/texts/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
methenyltetrahydrofolate cyclohydrolase activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005468`	occurs_in Activity compartment supported by an inspected functional annotation. ECO:0000269 (PubMed:3528153). Alternative isoenzymes may function elsewhere.	mitochondrion `GO:0005739`	UniProtKB:P09440 Source assertion: UniProtKB:P09440 describes the corresponding catalytic function in the mitochondrion; this projection applies to the activity enabled by SGD:S000000288. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/224/comments/0/texts/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
methylenetetrahydrofolate dehydrogenase (NADP+) activity `gomodel:YeastPathways_PWY3O-697/6a4c244800005484`	occurs_in Activity compartment supported by an inspected functional annotation. ECO:0000269 (PubMed:3528153). Alternative isoenzymes may function elsewhere.	mitochondrion `GO:0005739`	UniProtKB:P09440 Source assertion: UniProtKB:P09440 describes the corresponding catalytic function in the mitochondrion; this projection applies to the activity enabled by SGD:S000000288. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/224/comments/0/texts/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
MET12 Scer `SGD:S000005944`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250	FAD(3-) `CHEBI:57692`	UniProtKB:P46151 Source assertion: UniProtKB:P46151 annotates CHEBI:57692 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3848/comments/0/cofactors/0` UniProtKB:P46151 Source assertion: UniProtKB:P46151 has the exact SGD cross-reference SGD:S000005944. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3848/uniProtKBCrossReferences/0`
MET13 Scer `SGD:S000003093`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. Reviewed source qualification: Historical assignment as a mitochondrial ribosomal protein does not contradict the current FAD annotation; retain its similarity-based evidence grade. UniProt evidence: ECO:0000250	FAD(3-) `CHEBI:57692`	UniProtKB:P53128 Source assertion: UniProtKB:P53128 annotates CHEBI:57692 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3945/comments/0/cofactors/0` UniProtKB:P53128 Source assertion: UniProtKB:P53128 has the exact SGD cross-reference SGD:S000003093. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3945/uniProtKBCrossReferences/0`
SHM2 Scer `SGD:S000004048`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P34897)	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P37291 Source assertion: UniProtKB:P37291 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3551/comments/0/cofactors/0` UniProtKB:P37291 Source assertion: UniProtKB:P37291 has the exact SGD cross-reference SGD:S000004048. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3551/uniProtKBCrossReferences/0`
SHM1 Scer `SGD:S000000467`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: not specified	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P37292 Source assertion: UniProtKB:P37292 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1253/comments/0/cofactors/0` UniProtKB:P37292 Source assertion: UniProtKB:P37292 has the exact SGD cross-reference SGD:S000000467. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1253/uniProtKBCrossReferences/0`
Glycine cleavage system H protein, mitochondrial `UniProtKB:P39726`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. Reviewed source qualification: The source describes a covalently attached cofactor moiety. Keep that bound-state qualification; no free cosubstrate consumption is inferred. UniProt evidence: ECO:0000250 Binds 1 lipoyl cofactor covalently. UniProt note evidence: ECO:0000250	(R)-lipoate `CHEBI:83088`	UniProtKB:P39726 Source assertion: UniProtKB:P39726 annotates CHEBI:83088 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1589/comments/0/cofactors/0; /results/1589/comments/0/note`
LPD1 Scer `SGD:S000001876`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:9538259); ECO:0000269 (Reference:Ref.13) Binds 1 FAD per subunit. UniProt note evidence: ECO:0000269 (PubMed:9538259); ECO:0000269 (Reference:Ref.13)	FAD(3-) `CHEBI:57692`	UniProtKB:P09624 Source assertion: UniProtKB:P09624 annotates CHEBI:57692 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/227/comments/0/cofactors/0; /results/227/comments/0/note` UniProtKB:P09624 Source assertion: UniProtKB:P09624 has the exact SGD cross-reference SGD:S000001876. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/227/uniProtKBCrossReferences/0`
GCV2 Scer `SGD:S000004801`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250	pyridoxal 5'-phosphate(2-) `CHEBI:597326`	UniProtKB:P49095 Source assertion: UniProtKB:P49095 annotates CHEBI:597326 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2023/comments/0/cofactors/0` UniProtKB:P49095 Source assertion: UniProtKB:P49095 has the exact SGD cross-reference SGD:S000004801. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/2023/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_PWY3O-697 SGD GO-CAM folate interconversions - imported from: Saccharomyces Genome Database (source version: Pinned Noctua JSON archive; model date 2026-07-20; member ./YeastPathways_PWY3O-697.json)
SGD:S000218158 Complex Portal CPX-1268: Glycine decarboxylase multienzyme complex (source version: Inspected API snapshot 2026-10-05; release dates 2018-03-21)
UniProtKB:P09624 Reviewed UniProtKB P09624 SGD cross-reference (source version: UniProt release 2026_03)
UniProtKB:P49095 Reviewed UniProtKB P49095 SGD cross-reference (source version: UniProt release 2026_03)
UniProtKB:P48015 Reviewed UniProtKB P48015 SGD cross-reference (source version: UniProt release 2026_03)
UniProtKB:P09440 Reviewed UniProtKB P09440 location annotations (source version: UniProt release 2026_03)
UniProtKB:P07245 Reviewed UniProtKB P07245 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q02046 Reviewed UniProtKB Q02046 location annotations (source version: UniProt release 2026_03)
UniProtKB:P37291 Reviewed UniProtKB P37291 location annotations (source version: UniProt release 2026_03)
UniProtKB:P37292 Reviewed UniProtKB P37292 location annotations (source version: UniProt release 2026_03)
UniProtKB:P06785 Reviewed UniProtKB P06785 location annotations (source version: UniProt release 2026_03)
UniProtKB:P39726 Reviewed UniProtKB P39726 location annotations (source version: UniProt release 2026_03)
UniProtKB:P53128 UniProtKB P53128 catalytic annotations (source version: 2026_03)
PMID:11729203 Metabolic engineering in yeast demonstrates that S-adenosylmethionine controls flux through the methylenetetrahydrofolate reductase reaction in vivo
UniProtKB:P46151 UniProtKB P46151 cofactor annotations (source version: 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger. Reviewed exact Complex Portal composition, source evidence grades, and independent physical complex locations.
2026-10-05T06:17:04Z Codex; review-complex-composition; Reviewed exact Complex Portal composition, source evidence grades, and independent physical complex locations; reused existing proteins through reviewed UniProt SGD cross-references.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 14 activity location assignments and 13 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 15 independently supported location edges and quarantined 3 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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2026-10-05T07:03:07Z Codex
- EDIT — Resolve distinct folate states and MET13 nucleotide specificity
  Reviewed all14 activities against pinned UniProt2026_03 catalytic source and Rhea139. Corrected five methenyl endpoints formerly merged into methylene CHEBI20502; corrected MET13 NADPH/NADP using PMID11729203 while retaining independently supported MET12 NADH. Quarantined18 unsupported directed folate-flow assertions whose displayed chemical sides do not match; preserved full original edges in reports/causal_graph_review/folate-inositol-chemistry-review.json. No reaction reversal or compartment transport inferred. Semantic validation and six independent chemistry regression tests passed.
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