glutathione degradation

gomodel:YeastPathways_PWYQT-4432

Glutathione degradation uses ECM38 to transfer glutathione's gamma-glutamyl moiety to a proteinogenic amino acid, forming L-cysteinylglycine and an L-gamma-glutamyl-L-amino acid, then uses DUG1 to hydrolyze L-cysteinylglycine to L-cysteine and glycine in Saccharomyces cerevisiae S288C.

Pathway type

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

19 nodes · 20 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
glutathione catabolic process `GO:0006751`	biological process
L-cysteinylglycine zwitterion `CHEBI:61694`	small molecule
cytosol `GO:0005829`	cellular component
water `CHEBI:15377`	small molecule
glutathione `CHEBI:16856`	small molecule
DUG1 Scer `SGD:S000001940`	protein
L-cysteine zwitterion `CHEBI:35235`	small molecule
L-gamma-glutamyl-L-amino acid(1-) `CHEBI:71304`	small molecule
ECM38 Scer `SGD:S000004290`	protein
proteinogenic amino acid `CHEBI:83813`	small molecule
glycine zwitterion `CHEBI:57305`	small molecule
cytoplasm `GO:0005737`	cellular component
mitochondrion `GO:0005739`	cellular component
vacuolar membrane `GO:0005774`	cellular component
vacuole `GO:0005773`	cellular component
zinc(2+) `CHEBI:29105`	cofactor
manganese(2+) `CHEBI:29035`	cofactor
glutathione gamma-glutamate hydrolase `gomodel:RXN-6601`	molecular activity
metallodipeptidase activity `gomodel:RXN-6622`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
glutathione gamma-glutamate hydrolase `gomodel:RXN-6601`	part_of	glutathione catabolic process `GO:0006751`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6601 BFO:0000050 gomodel:YeastPathways_PWYQT-4432/YeastPathways_PWYQT-4432. Source location `./YeastPathways_PWYQT-4432.json#/facts/0; endpoint types: /individuals/6/type; /individuals/23/type`
glutathione gamma-glutamate hydrolase `gomodel:RXN-6601`	has_output	L-cysteinylglycine zwitterion `CHEBI:61694`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6601 RO:0002234 gomodel:CHEBI_61694_RXN-6601. Source location `./YeastPathways_PWYQT-4432.json#/facts/1; endpoint types: /individuals/6/type; /individuals/4/type`
metallodipeptidase activity `gomodel:RXN-6622`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6622 BFO:0000066 gomodel:reaction_RXN-6622_location_lociGO_0005829. Source location `./YeastPathways_PWYQT-4432.json#/facts/2; endpoint types: /individuals/7/type; /individuals/21/type`
metallodipeptidase activity `gomodel:RXN-6622`	has_input	water `CHEBI:15377`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6622 RO:0002233 gomodel:CHEBI_15377_RXN-6622. Source location `./YeastPathways_PWYQT-4432.json#/facts/3; endpoint types: /individuals/7/type; /individuals/0/type`
glutathione gamma-glutamate hydrolase `gomodel:RXN-6601`	has_input	glutathione `CHEBI:16856`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6601 RO:0002233 gomodel:CHEBI_57925_RXN-6601. Source location `./YeastPathways_PWYQT-4432.json#/facts/4; endpoint types: /individuals/6/type; /individuals/3/type`
metallodipeptidase activity `gomodel:RXN-6622`	has_input	L-cysteinylglycine zwitterion `CHEBI:61694`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6622 RO:0002233 gomodel:CHEBI_61694_RXN-6601. Source location `./YeastPathways_PWYQT-4432.json#/facts/5; endpoint types: /individuals/7/type; /individuals/4/type`
DUG1 Scer `SGD:S000001940`	enables	metallodipeptidase activity `gomodel:RXN-6622`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6622 RO:0002333 gomodel:YeastPathways_PWYQT-4432/6a6bcaed00000235. Source location `./YeastPathways_PWYQT-4432.json#/facts/7; endpoint types: /individuals/7/type; /individuals/24/type`
metallodipeptidase activity `gomodel:RXN-6622`	has_output	L-cysteine zwitterion `CHEBI:35235`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6622 RO:0002234 gomodel:CHEBI_35235_RXN-6622. Source location `./YeastPathways_PWYQT-4432.json#/facts/8; endpoint types: /individuals/7/type; /individuals/1/type`
glutathione gamma-glutamate hydrolase `gomodel:RXN-6601`	has_output	L-gamma-glutamyl-L-amino acid(1-) `CHEBI:71304`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6601 RO:0002234 gomodel:53e455f9-a234-432e-ade1-7720e76926f9_RXN-6601. Source location `./YeastPathways_PWYQT-4432.json#/facts/9; endpoint types: /individuals/6/type; /individuals/22/type`
metallodipeptidase activity `gomodel:RXN-6622`	part_of	glutathione catabolic process `GO:0006751`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6622 BFO:0000050 gomodel:YeastPathways_PWYQT-4432/YeastPathways_PWYQT-4432. Source location `./YeastPathways_PWYQT-4432.json#/facts/10; endpoint types: /individuals/7/type; /individuals/23/type`
ECM38 Scer `SGD:S000004290`	enables	glutathione gamma-glutamate hydrolase `gomodel:RXN-6601`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6601 RO:0002333 gomodel:YLR299W-MONOMER_RXN-6601_controller. Source location `./YeastPathways_PWYQT-4432.json#/facts/11; endpoint types: /individuals/6/type; /individuals/8/type`
glutathione gamma-glutamate hydrolase `gomodel:RXN-6601`	provides_input_for	metallodipeptidase activity `gomodel:RXN-6622`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6601 RO:0002413 gomodel:RXN-6622. Source location `./YeastPathways_PWYQT-4432.json#/facts/12; endpoint types: /individuals/6/type; /individuals/7/type`
glutathione gamma-glutamate hydrolase `gomodel:RXN-6601`	has_input	proteinogenic amino acid `CHEBI:83813`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6601 RO:0002233 gomodel:CHEBI_83813_RXN-6601. Source location `./YeastPathways_PWYQT-4432.json#/facts/13; endpoint types: /individuals/6/type; /individuals/5/type`
metallodipeptidase activity `gomodel:RXN-6622`	has_output	glycine zwitterion `CHEBI:57305`	gomodel:YeastPathways_PWYQT-4432 Source assertion: The model asserts gomodel:RXN-6622 RO:0002234 gomodel:CHEBI_57305_RXN-6622. Source location `./YeastPathways_PWYQT-4432.json#/facts/14; endpoint types: /individuals/7/type; /individuals/2/type`
DUG1 Scer `SGD:S000001940`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095); ECO:0000269 (PubMed:17179087)	cytoplasm `GO:0005737`	UniProtKB:P43616 Source assertion: UniProtKB:P43616 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1878/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P43616 Source assertion: UniProtKB:P43616 has the exact SGD cross-reference SGD:S000001940. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1878/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
DUG1 Scer `SGD:S000001940`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:16823961)	mitochondrion `GO:0005739`	UniProtKB:P43616 Source assertion: UniProtKB:P43616 annotates location Mitochondrion (SL-0173). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1878/comments/1/subcellularLocations/1; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P43616 Source assertion: UniProtKB:P43616 has the exact SGD cross-reference SGD:S000001940. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1878/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
ECM38 Scer `SGD:S000004290`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:11672438); ECO:0000269 (PubMed:6143552); ECO:0000269 (PubMed:6143574) Topology: Single-pass type II membrane protein.	vacuolar membrane `GO:0005774`	UniProtKB:Q05902 Source assertion: UniProtKB:Q05902 annotates location Vacuole membrane (SL-0271). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2691/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:Q05902 Source assertion: UniProtKB:Q05902 has the exact SGD cross-reference SGD:S000004290. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2691/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
glutathione gamma-glutamate hydrolase `gomodel:RXN-6601`	occurs_in Activity compartment supported by an inspected functional annotation. ECO:0000269 (PubMed:11672438). Alternative isoenzymes may function elsewhere.	vacuole `GO:0005773`	UniProtKB:Q05902 Source assertion: UniProtKB:Q05902 describes the corresponding catalytic function in the vacuole; this projection applies to the activity enabled by SGD:S000004290. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/2691/comments/0/texts/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
DUG1 Scer `SGD:S000001940`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:19346245)	zinc(2+) `CHEBI:29105`	UniProtKB:P43616 Source assertion: UniProtKB:P43616 annotates CHEBI:29105 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1878/comments/0/cofactors/0` UniProtKB:P43616 Source assertion: UniProtKB:P43616 has the exact SGD cross-reference SGD:S000001940. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1878/uniProtKBCrossReferences/0`
DUG1 Scer `SGD:S000001940`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:19346245)	manganese(2+) `CHEBI:29035`	UniProtKB:P43616 Source assertion: UniProtKB:P43616 annotates CHEBI:29035 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1878/comments/0/cofactors/1` UniProtKB:P43616 Source assertion: UniProtKB:P43616 has the exact SGD cross-reference SGD:S000001940. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1878/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_PWYQT-4432 GO-CAM glutathione degradation model YeastPathways_PWYQT-4432 (source version: Pinned Noctua JSON archive; model date 2026-08-03; member ./YeastPathways_PWYQT-4432.json)
UniProtKB:P43616 Reviewed UniProtKB P43616 location annotations (source version: UniProt release 2026_03)
UniProtKB:Q05902 Reviewed UniProtKB Q05902 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 2 activity location assignments and 2 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 4 independently supported location edges and quarantined 1 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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