pyruvate decarboxylation to acetyl CoA

gomodel:YeastPathways_PYRUVDEHYD-PWY

Pyruvate decarboxylation to acetyl CoA in Saccharomyces cerevisiae S288C uses pyruvate dehydrogenase activities to transfer acetyl groups through lipoyl-lysine intermediates, form acetyl-CoA, and regenerate oxidized lipoyl cofactor while reducing NAD(+) to NADH.

Pathway type

central carbon metabolism

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

25 nodes · 36 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

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Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
pyruvate `CHEBI:15361`	small molecule
pyruvate decarboxylation to acetyl-CoA `GO:0006086`	biological process
hydron `CHEBI:15378`	small molecule
carbon dioxide `CHEBI:16526`	small molecule
Enzyme N6-(lipoyl)lysine `CHEBI:80218`	small molecule
cytosol `GO:0005829`	cellular component
[Dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine `CHEBI:80404`	small molecule
Enzyme N6-(dihydrolipoyl)lysine `CHEBI:80219`	small molecule
LAT1 Scer `SGD:S000005015`	protein
acetyl-CoA(4-) `CHEBI:57288`	small molecule
LPD1 Scer `SGD:S000001876`	protein
NAD(1-) `CHEBI:57540`	small molecule
PDB1 Scer `SGD:S000000425`	protein
NADH(2-) `CHEBI:57945`	small molecule
PDA1 Scer `SGD:S000000980`	protein
coenzyme A(4-) `CHEBI:57287`	small molecule
mitochondrial matrix `GO:0005759`	cellular component
(R)-lipoate `CHEBI:83088`	cofactor
FAD(3-) `CHEBI:57692`	cofactor
thiamine(1+) diphosphate(3-) `CHEBI:58937`	cofactor
magnesium(2+) `CHEBI:18420`	cofactor
a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine + NAD⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + NADH + H⁺ `gomodel:RXN0-1132`	molecular activity
acetyl-CoA + a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine <- a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + coenzyme A `gomodel:RXN0-1133`	molecular activity
pyruvate + a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + H⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + CO₂ `gomodel:RXN0-1134`	molecular activity
pyruvate dehydrogenase (acetyl-transferring) activity `gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
pyruvate dehydrogenase (acetyl-transferring) activity `gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320`	has_input	pyruvate `CHEBI:15361`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320 RO:0002233 gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000322. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/0; endpoint types: /individuals/39/type; /individuals/41/type`
pyruvate + a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + H⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + CO₂ `gomodel:RXN0-1134`	part_of	pyruvate decarboxylation to acetyl-CoA `GO:0006086`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1134 BFO:0000050 gomodel:YeastPathways_PYRUVDEHYD-PWY/YeastPathways_PYRUVDEHYD-PWY. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/1; endpoint types: /individuals/13/type; /individuals/38/type`
pyruvate dehydrogenase (acetyl-transferring) activity `gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320 RO:0002233 gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000321. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/2; endpoint types: /individuals/39/type; /individuals/40/type`
pyruvate + a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + H⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + CO₂ `gomodel:RXN0-1134`	has_output	carbon dioxide `CHEBI:16526`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1134 RO:0002234 gomodel:CHEBI_16526_RXN0-1134. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/3; endpoint types: /individuals/13/type; /individuals/3/type`
a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine + NAD⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + NADH + H⁺ `gomodel:RXN0-1132`	part_of	pyruvate decarboxylation to acetyl-CoA `GO:0006086`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1132 BFO:0000050 gomodel:YeastPathways_PYRUVDEHYD-PWY/YeastPathways_PYRUVDEHYD-PWY. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/4; endpoint types: /individuals/11/type; /individuals/38/type`
pyruvate dehydrogenase (acetyl-transferring) activity `gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320`	has_input	Enzyme N6-(lipoyl)lysine `CHEBI:80218`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320 RO:0002233 gomodel:CHEBI_80218_RXN0-1132. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/5; endpoint types: /individuals/39/type; /individuals/8/type`
pyruvate + a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + H⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + CO₂ `gomodel:RXN0-1134`	has_input	Enzyme N6-(lipoyl)lysine `CHEBI:80218`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1134 RO:0002233 gomodel:CHEBI_80218_RXN0-1132. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/6; endpoint types: /individuals/13/type; /individuals/8/type`
pyruvate dehydrogenase (acetyl-transferring) activity `gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320`	part_of	pyruvate decarboxylation to acetyl-CoA `GO:0006086`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320 BFO:0000050 gomodel:YeastPathways_PYRUVDEHYD-PWY/YeastPathways_PYRUVDEHYD-PWY. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/8; endpoint types: /individuals/39/type; /individuals/38/type`
pyruvate dehydrogenase (acetyl-transferring) activity `gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320`	has_output	[Dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine `CHEBI:80404`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320 RO:0002234 gomodel:CHEBI_80404_RXN0-1134. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/9; endpoint types: /individuals/39/type; /individuals/10/type`
a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine + NAD⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + NADH + H⁺ `gomodel:RXN0-1132`	has_input	Enzyme N6-(dihydrolipoyl)lysine `CHEBI:80219`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1132 RO:0002233 gomodel:CHEBI_80219_RXN0-1133. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/10; endpoint types: /individuals/11/type; /individuals/9/type`
acetyl-CoA + a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine <- a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + coenzyme A `gomodel:RXN0-1133`	part_of	pyruvate decarboxylation to acetyl-CoA `GO:0006086`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1133 BFO:0000050 gomodel:YeastPathways_PYRUVDEHYD-PWY/YeastPathways_PYRUVDEHYD-PWY. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/13; endpoint types: /individuals/12/type; /individuals/38/type`
pyruvate dehydrogenase (acetyl-transferring) activity `gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320`	has_output	carbon dioxide `CHEBI:16526`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320 RO:0002234 gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000323. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/14; endpoint types: /individuals/39/type; /individuals/42/type`
LAT1 Scer `SGD:S000005015`	enables	acetyl-CoA + a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine <- a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + coenzyme A `gomodel:RXN0-1133`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1133 RO:0002333 gomodel:YNL071W-MONOMER_RXN0-1133_controller. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/15; endpoint types: /individuals/12/type; /individuals/15/type`
pyruvate + a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + H⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + CO₂ `gomodel:RXN0-1134`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1134 RO:0002233 gomodel:CHEBI_15378_RXN0-1134. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/16; endpoint types: /individuals/13/type; /individuals/2/type`
acetyl-CoA + a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine <- a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + coenzyme A `gomodel:RXN0-1133`	has_output	acetyl-CoA(4-) `CHEBI:57288`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1133 RO:0002234 gomodel:CHEBI_57288_RXN0-1133. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/17; endpoint types: /individuals/12/type; /individuals/5/type`
a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine + NAD⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + NADH + H⁺ `gomodel:RXN0-1132`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1132 RO:0002234 gomodel:CHEBI_15378_RXN0-1132. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/18; endpoint types: /individuals/11/type; /individuals/1/type`
acetyl-CoA + a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine <- a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + coenzyme A `gomodel:RXN0-1133`	has_input	[Dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine `CHEBI:80404`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1133 RO:0002233 gomodel:CHEBI_80404_RXN0-1134. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/19; endpoint types: /individuals/12/type; /individuals/10/type`
LPD1 Scer `SGD:S000001876`	enables	a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine + NAD⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + NADH + H⁺ `gomodel:RXN0-1132`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1132 RO:0002333 gomodel:YFL018C-MONOMER_RXN0-1132_controller. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/20; endpoint types: /individuals/11/type; /individuals/14/type`
a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine + NAD⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + NADH + H⁺ `gomodel:RXN0-1132`	has_output	Enzyme N6-(lipoyl)lysine `CHEBI:80218`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1132 RO:0002234 gomodel:CHEBI_80218_RXN0-1132. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/21; endpoint types: /individuals/11/type; /individuals/8/type`
acetyl-CoA + a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine <- a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + coenzyme A `gomodel:RXN0-1133`	has_output	Enzyme N6-(dihydrolipoyl)lysine `CHEBI:80219`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1133 RO:0002234 gomodel:CHEBI_80219_RXN0-1133. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/22; endpoint types: /individuals/12/type; /individuals/9/type`
a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine + NAD⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + NADH + H⁺ `gomodel:RXN0-1132`	has_input	NAD(1-) `CHEBI:57540`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1132 RO:0002233 gomodel:CHEBI_57540_RXN0-1132. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/23; endpoint types: /individuals/11/type; /individuals/6/type`
PDB1 Scer `SGD:S000000425`	enables	pyruvate dehydrogenase (acetyl-transferring) activity `gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000320 RO:0002333 gomodel:YeastPathways_PYRUVDEHYD-PWY/6a4c244800000324. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/24; endpoint types: /individuals/39/type; /individuals/43/type`
pyruvate + a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + H⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + CO₂ `gomodel:RXN0-1134`	has_input	pyruvate `CHEBI:15361`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1134 RO:0002233 gomodel:CHEBI_15361_RXN0-1134. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/26; endpoint types: /individuals/13/type; /individuals/0/type`
a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine + NAD⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + NADH + H⁺ `gomodel:RXN0-1132`	has_output	NADH(2-) `CHEBI:57945`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1132 RO:0002234 gomodel:CHEBI_57945_RXN0-1132. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/27; endpoint types: /individuals/11/type; /individuals/7/type`
pyruvate + a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + H⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + CO₂ `gomodel:RXN0-1134`	has_output	[Dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine `CHEBI:80404`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1134 RO:0002234 gomodel:CHEBI_80404_RXN0-1134. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/28; endpoint types: /individuals/13/type; /individuals/10/type`
PDA1 Scer `SGD:S000000980`	enables	pyruvate + a [pyruvate dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + H⁺ -> a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + CO₂ `gomodel:RXN0-1134`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1134 RO:0002333 gomodel:YeastPathways_PYRUVDEHYD-PWY/6690711d00000754. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/29; endpoint types: /individuals/13/type; /individuals/53/type`
acetyl-CoA + a [pyruvate dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine <- a [pyruvate dehydrogenase E2 protein] N⁶-S-acetyldihydrolipoyl-L-lysine + coenzyme A `gomodel:RXN0-1133`	has_input	coenzyme A(4-) `CHEBI:57287`	gomodel:YeastPathways_PYRUVDEHYD-PWY Source assertion: The model asserts gomodel:RXN0-1133 RO:0002233 gomodel:CHEBI_57287_RXN0-1133. Source location `./YeastPathways_PYRUVDEHYD-PWY.json#/facts/30; endpoint types: /individuals/12/type; /individuals/4/type`
LAT1 Scer `SGD:S000005015`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	mitochondrial matrix `GO:0005759`	UniProtKB:P12695 Source assertion: UniProtKB:P12695 annotates location Mitochondrion matrix (SL-0170). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/350/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P12695 Source assertion: UniProtKB:P12695 has the exact SGD cross-reference SGD:S000005015. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/350/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
LPD1 Scer `SGD:S000001876`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	mitochondrial matrix `GO:0005759`	UniProtKB:P09624 Source assertion: UniProtKB:P09624 annotates location Mitochondrion matrix (SL-0170). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/227/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P09624 Source assertion: UniProtKB:P09624 has the exact SGD cross-reference SGD:S000001876. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/227/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
PDB1 Scer `SGD:S000000425`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	mitochondrial matrix `GO:0005759`	UniProtKB:P32473 Source assertion: UniProtKB:P32473 annotates location Mitochondrion matrix (SL-0170). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3420/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P32473 Source assertion: UniProtKB:P32473 has the exact SGD cross-reference SGD:S000000425. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/3420/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
PDA1 Scer `SGD:S000000980`	located_in Physical protein location from reviewed UniProt. evidence code not specified by source	mitochondrial matrix `GO:0005759`	UniProtKB:P16387 Source assertion: UniProtKB:P16387 annotates location Mitochondrion matrix (SL-0170). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/437/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P16387 Source assertion: UniProtKB:P16387 has the exact SGD cross-reference SGD:S000000980. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/437/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
LAT1 Scer `SGD:S000005015`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. Reviewed source qualification: The source describes a covalently attached cofactor moiety. Keep that bound-state qualification; no free cosubstrate consumption is inferred. UniProt evidence: not specified Binds 1 lipoyl cofactor covalently. UniProt note evidence: ECO:0000269 (PubMed:3050999)	(R)-lipoate `CHEBI:83088`	UniProtKB:P12695 Source assertion: UniProtKB:P12695 annotates CHEBI:83088 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/350/comments/0/cofactors/0; /results/350/comments/0/note` UniProtKB:P12695 Source assertion: UniProtKB:P12695 has the exact SGD cross-reference SGD:S000005015. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/350/uniProtKBCrossReferences/0`
LPD1 Scer `SGD:S000001876`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000269 (PubMed:9538259); ECO:0000269 (Reference:Ref.13) Binds 1 FAD per subunit. UniProt note evidence: ECO:0000269 (PubMed:9538259); ECO:0000269 (Reference:Ref.13)	FAD(3-) `CHEBI:57692`	UniProtKB:P09624 Source assertion: UniProtKB:P09624 annotates CHEBI:57692 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/227/comments/0/cofactors/0; /results/227/comments/0/note` UniProtKB:P09624 Source assertion: UniProtKB:P09624 has the exact SGD cross-reference SGD:S000001876. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/227/uniProtKBCrossReferences/0`
PDB1 Scer `SGD:S000000425`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P11177)	thiamine(1+) diphosphate(3-) `CHEBI:58937`	UniProtKB:P32473 Source assertion: UniProtKB:P32473 annotates CHEBI:58937 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3420/comments/0/cofactors/0` UniProtKB:P32473 Source assertion: UniProtKB:P32473 has the exact SGD cross-reference SGD:S000000425. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3420/uniProtKBCrossReferences/0`
PDA1 Scer `SGD:S000000980`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P08559)	thiamine(1+) diphosphate(3-) `CHEBI:58937`	UniProtKB:P16387 Source assertion: UniProtKB:P16387 annotates CHEBI:58937 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/437/comments/0/cofactors/0` UniProtKB:P16387 Source assertion: UniProtKB:P16387 has the exact SGD cross-reference SGD:S000000980. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/437/uniProtKBCrossReferences/0`
PDA1 Scer `SGD:S000000980`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 (UniProtKB:P08559)	magnesium(2+) `CHEBI:18420`	UniProtKB:P16387 Source assertion: UniProtKB:P16387 annotates CHEBI:18420 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/437/comments/0/cofactors/1` UniProtKB:P16387 Source assertion: UniProtKB:P16387 has the exact SGD cross-reference SGD:S000000980. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/437/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_PYRUVDEHYD-PWY SGD GO-CAM pyruvate decarboxylation to acetyl CoA - imported from: Saccharomyces Genome Database (source version: Pinned Noctua JSON archive; model date 2026-07-07; member ./YeastPathways_PYRUVDEHYD-PWY.json)
UniProtKB:P12695 Reviewed UniProtKB P12695 location annotations (source version: UniProt release 2026_03)
UniProtKB:P09624 Reviewed UniProtKB P09624 location annotations (source version: UniProt release 2026_03)
UniProtKB:P32473 Reviewed UniProtKB P32473 location annotations (source version: UniProt release 2026_03)
UniProtKB:P16387 Reviewed UniProtKB P16387 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 4 activity location assignments and 4 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 4 independently supported location edges and quarantined 4 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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