assimilatory sulfate reduction I

gomodel:YeastPathways_SO4ASSIM-PWY

Saccharomyces cerevisiae S288C assimilatory sulfate reduction route that converts sulfate to hydrogen sulfide through adenosine 5'-phosphosulfate, 3'-phosphoadenylyl-sulfate, and sulfite.

Pathway type

metabolic

Organism scope

Saccharomyces cerevisiae S288C NCBITaxon:559292

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Pathway network

33 nodes · 50 asserted directed edges

Arrows show recorded subject → object relationships, not necessarily metabolic flow. Select a node for details or an arrow for evidence. Scroll, zoom or find a node to explore.

Reaction / activity
Chemical / cofactor
Protein / complex
Cell / process context
Solid arrow: mechanistic relation
Dashed arrow: membership, location or cofactor relation

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Components and activities

Declared biological components and source reaction directions
Node	Kind or role	Source direction
Saccharomyces cerevisiae S288C `NCBITaxon:559292`	taxon
hydrogen sulfide `CHEBI:16136`	small molecule
MET14 Scer `SGD:S000001484`	protein
NADPH(4-) `CHEBI:57783`	small molecule
water `CHEBI:15377`	small molecule
hydron `CHEBI:15378`	small molecule
MET3 Scer `SGD:S000003771`	protein
sulfite `CHEBI:17359`	small molecule
cytosol `GO:0005829`	cellular component
sulfate assimilation `GO:0000103`	biological process
CPX-3163 Scer `SGD:S000217821`	complex
NADP(3-) `CHEBI:58349`	small molecule
5'-adenylyl sulfate(2-) `CHEBI:58243`	small molecule
MET10 Scer `SGD:S000001926`	protein
thioredoxin `CHEBI:15033`	protein
adenosine 3',5'-bismonophosphate(4-) `CHEBI:58343`	small molecule
ATP(4-) `CHEBI:30616`	small molecule
3'-phosphonato-5'-adenylyl sulfate(4-) `CHEBI:58339`	small molecule
MET5 Scer `SGD:S000003898`	protein
sulfate `CHEBI:16189`	small molecule
diphosphoric acid `CHEBI:29888`	small molecule
ADP(3-) `CHEBI:456216`	small molecule
MET16 Scer `SGD:S000006371`	protein
Fe4S4 iron-sulfur cluster `CHEBI:64607`	cofactor
FAD(3-) `CHEBI:57692`	cofactor
FMN(3-) `CHEBI:58210`	cofactor
siroheme `CHEBI:28599`	cofactor
cytoplasm `GO:0005737`	cellular component
siroheme(8-) `CHEBI:60052`	cofactor
tetra-mu3-sulfido-tetrairon `CHEBI:49883`	cofactor
adenosine 3',5'-bisphosphate + sulfite + an oxidized thioredoxin + 2 H⁺ <=> 3'-phosphoadenylyl-sulfate + a reduced thioredoxin `gomodel:1.8.4.8-RXN`	molecular activity
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	molecular activity
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	molecular activity
hydrogen sulfide + 3 NADP⁺ + 3 H₂O <- sulfite + 3 NADPH + 5 H⁺ `gomodel:SULFITE-REDUCT-RXN`	molecular activity

Mechanistic edges

Mechanistic edges and their cited evidence
Subject	Predicate and description	Object	Evidence
hydrogen sulfide + 3 NADP⁺ + 3 H₂O <- sulfite + 3 NADPH + 5 H⁺ `gomodel:SULFITE-REDUCT-RXN`	has_output	hydrogen sulfide `CHEBI:16136`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFITE-REDUCT-RXN RO:0002234 gomodel:CHEBI_16136_SULFITE-REDUCT-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/0; endpoint types: /individuals/22/type; /individuals/6/type`
MET14 Scer `SGD:S000001484`	enables	adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002333 gomodel:YKL001C-MONOMER_ADENYLYLSULFKIN-RXN_controller. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/1; endpoint types: /individuals/0/type; /individuals/24/type`
hydrogen sulfide + 3 NADP⁺ + 3 H₂O <- sulfite + 3 NADPH + 5 H⁺ `gomodel:SULFITE-REDUCT-RXN`	has_input	NADPH(4-) `CHEBI:57783`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFITE-REDUCT-RXN RO:0002233 gomodel:CHEBI_57783_SULFITE-REDUCT-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/2; endpoint types: /individuals/22/type; /individuals/13/type`
hydrogen sulfide + 3 NADP⁺ + 3 H₂O <- sulfite + 3 NADPH + 5 H⁺ `gomodel:SULFITE-REDUCT-RXN`	has_output	water `CHEBI:15377`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFITE-REDUCT-RXN RO:0002234 gomodel:CHEBI_15377_SULFITE-REDUCT-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/3; endpoint types: /individuals/22/type; /individuals/1/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002234 gomodel:CHEBI_15378_ADENYLYLSULFKIN-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/4; endpoint types: /individuals/0/type; /individuals/3/type`
MET3 Scer `SGD:S000003771`	enables	sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002333 gomodel:YJR010W-MONOMER_SULFATE-ADENYLYLTRANS-RXN_controller. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/5; endpoint types: /individuals/21/type; /individuals/23/type`
adenosine 3',5'-bisphosphate + sulfite + an oxidized thioredoxin + 2 H⁺ <=> 3'-phosphoadenylyl-sulfate + a reduced thioredoxin `gomodel:1.8.4.8-RXN`	has_output	sulfite `CHEBI:17359`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:1.8.4.8-RXN RO:0002234 gomodel:CHEBI_17359_1.8.4.8-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/6; endpoint types: /individuals/60/type; /individuals/8/type`
adenosine 3',5'-bisphosphate + sulfite + an oxidized thioredoxin + 2 H⁺ <=> 3'-phosphoadenylyl-sulfate + a reduced thioredoxin `gomodel:1.8.4.8-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:1.8.4.8-RXN BFO:0000066 gomodel:reaction_1.8.4.8-RXN_location_lociGO_0005829. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/7; endpoint types: /individuals/60/type; /individuals/56/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002233 gomodel:CHEBI_15378_SULFATE-ADENYLYLTRANS-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/8; endpoint types: /individuals/21/type; /individuals/4/type`
adenosine 3',5'-bisphosphate + sulfite + an oxidized thioredoxin + 2 H⁺ <=> 3'-phosphoadenylyl-sulfate + a reduced thioredoxin `gomodel:1.8.4.8-RXN`	part_of	sulfate assimilation `GO:0000103`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:1.8.4.8-RXN BFO:0000050 gomodel:YeastPathways_SO4ASSIM-PWY/YeastPathways_SO4ASSIM-PWY. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/9; endpoint types: /individuals/60/type; /individuals/61/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	provides_input_for	adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002413 gomodel:ADENYLYLSULFKIN-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/10; endpoint types: /individuals/21/type; /individuals/0/type`
adenosine 3',5'-bisphosphate + sulfite + an oxidized thioredoxin + 2 H⁺ <=> 3'-phosphoadenylyl-sulfate + a reduced thioredoxin `gomodel:1.8.4.8-RXN`	has_output	hydron `CHEBI:15378`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:1.8.4.8-RXN RO:0002234 gomodel:CHEBI_15378_1.8.4.8-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/11; endpoint types: /individuals/60/type; /individuals/2/type`
hydrogen sulfide + 3 NADP⁺ + 3 H₂O <- sulfite + 3 NADPH + 5 H⁺ `gomodel:SULFITE-REDUCT-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFITE-REDUCT-RXN BFO:0000066 gomodel:reaction_SULFITE-REDUCT-RXN_location_lociGO_0005829. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/12; endpoint types: /individuals/22/type; /individuals/59/type`
CPX-3163 Scer `SGD:S000217821`	enables	hydrogen sulfide + 3 NADP⁺ + 3 H₂O <- sulfite + 3 NADPH + 5 H⁺ `gomodel:SULFITE-REDUCT-RXN`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFITE-REDUCT-RXN RO:0002333 gomodel:CPLX3O-23_SULFITE-REDUCT-RXN_controller. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/13; endpoint types: /individuals/22/type; /individuals/18/type`
hydrogen sulfide + 3 NADP⁺ + 3 H₂O <- sulfite + 3 NADPH + 5 H⁺ `gomodel:SULFITE-REDUCT-RXN`	has_output	NADP(3-) `CHEBI:58349`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFITE-REDUCT-RXN RO:0002234 gomodel:CHEBI_58349_SULFITE-REDUCT-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/14; endpoint types: /individuals/22/type; /individuals/17/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	provides_input_for	adenosine 3',5'-bisphosphate + sulfite + an oxidized thioredoxin + 2 H⁺ <=> 3'-phosphoadenylyl-sulfate + a reduced thioredoxin `gomodel:1.8.4.8-RXN`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002413 gomodel:1.8.4.8-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/15; endpoint types: /individuals/0/type; /individuals/60/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	has_output	5'-adenylyl sulfate(2-) `CHEBI:58243`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002234 gomodel:CHEBI_58243_SULFATE-ADENYLYLTRANS-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/16; endpoint types: /individuals/21/type; /individuals/14/type`
CPX-3163 Scer `SGD:S000217821`	has_part	MET10 Scer `SGD:S000001926`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:CPLX3O-23_SULFITE-REDUCT-RXN_controller BFO:0000051 gomodel:SGD_S000001926_CPLX3O-23_component. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/17; endpoint types: /individuals/18/type; /individuals/19/type`
hydrogen sulfide + 3 NADP⁺ + 3 H₂O <- sulfite + 3 NADPH + 5 H⁺ `gomodel:SULFITE-REDUCT-RXN`	has_input	sulfite `CHEBI:17359`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFITE-REDUCT-RXN RO:0002233 gomodel:CHEBI_17359_1.8.4.8-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/18; endpoint types: /individuals/22/type; /individuals/8/type`
adenosine 3',5'-bisphosphate + sulfite + an oxidized thioredoxin + 2 H⁺ <=> 3'-phosphoadenylyl-sulfate + a reduced thioredoxin `gomodel:1.8.4.8-RXN`	has_input	thioredoxin `CHEBI:15033`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:1.8.4.8-RXN RO:0002233 gomodel:YeastPathways_SO4ASSIM-PWY/6a4c244800003900. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/19; endpoint types: /individuals/60/type; /individuals/62/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN BFO:0000066 gomodel:reaction_SULFATE-ADENYLYLTRANS-RXN_location_lociGO_0005829. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/20; endpoint types: /individuals/21/type; /individuals/58/type`
adenosine 3',5'-bisphosphate + sulfite + an oxidized thioredoxin + 2 H⁺ <=> 3'-phosphoadenylyl-sulfate + a reduced thioredoxin `gomodel:1.8.4.8-RXN`	has_output	adenosine 3',5'-bismonophosphate(4-) `CHEBI:58343`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:1.8.4.8-RXN RO:0002234 gomodel:CHEBI_58343_1.8.4.8-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/21; endpoint types: /individuals/60/type; /individuals/16/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002233 gomodel:CHEBI_30616_SULFATE-ADENYLYLTRANS-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/22; endpoint types: /individuals/21/type; /individuals/11/type`
adenosine 3',5'-bisphosphate + sulfite + an oxidized thioredoxin + 2 H⁺ <=> 3'-phosphoadenylyl-sulfate + a reduced thioredoxin `gomodel:1.8.4.8-RXN`	has_output	thioredoxin `CHEBI:15033`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:1.8.4.8-RXN RO:0002234 gomodel:YeastPathways_SO4ASSIM-PWY/6a4c244800003901. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/23; endpoint types: /individuals/60/type; /individuals/63/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	has_output	3'-phosphonato-5'-adenylyl sulfate(4-) `CHEBI:58339`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002234 gomodel:CHEBI_58339_ADENYLYLSULFKIN-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/24; endpoint types: /individuals/0/type; /individuals/15/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	occurs_in	cytosol `GO:0005829`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN BFO:0000066 gomodel:reaction_ADENYLYLSULFKIN-RXN_location_lociGO_0005829. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/25; endpoint types: /individuals/0/type; /individuals/57/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	has_input	5'-adenylyl sulfate(2-) `CHEBI:58243`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002233 gomodel:CHEBI_58243_SULFATE-ADENYLYLTRANS-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/26; endpoint types: /individuals/0/type; /individuals/14/type`
CPX-3163 Scer `SGD:S000217821`	has_part	MET5 Scer `SGD:S000003898`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:CPLX3O-23_SULFITE-REDUCT-RXN_controller BFO:0000051 gomodel:SGD_S000003898_CPLX3O-23_component. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/27; endpoint types: /individuals/18/type; /individuals/20/type`
hydrogen sulfide + 3 NADP⁺ + 3 H₂O <- sulfite + 3 NADPH + 5 H⁺ `gomodel:SULFITE-REDUCT-RXN`	has_input	hydron `CHEBI:15378`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFITE-REDUCT-RXN RO:0002233 gomodel:CHEBI_15378_SULFITE-REDUCT-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/28; endpoint types: /individuals/22/type; /individuals/5/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	has_input	sulfate `CHEBI:16189`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002233 gomodel:CHEBI_16189_SULFATE-ADENYLYLTRANS-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/29; endpoint types: /individuals/21/type; /individuals/7/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	part_of	sulfate assimilation `GO:0000103`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN BFO:0000050 gomodel:YeastPathways_SO4ASSIM-PWY/YeastPathways_SO4ASSIM-PWY. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/30; endpoint types: /individuals/0/type; /individuals/61/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	has_output	diphosphoric acid `CHEBI:29888`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN RO:0002234 gomodel:CHEBI_29888_SULFATE-ADENYLYLTRANS-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/31; endpoint types: /individuals/21/type; /individuals/9/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	has_output	ADP(3-) `CHEBI:456216`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002234 gomodel:CHEBI_456216_ADENYLYLSULFKIN-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/32; endpoint types: /individuals/0/type; /individuals/12/type`
adenosine 3',5'-bisphosphate + sulfite + an oxidized thioredoxin + 2 H⁺ <=> 3'-phosphoadenylyl-sulfate + a reduced thioredoxin `gomodel:1.8.4.8-RXN`	has_input	3'-phosphonato-5'-adenylyl sulfate(4-) `CHEBI:58339`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:1.8.4.8-RXN RO:0002233 gomodel:CHEBI_58339_ADENYLYLSULFKIN-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/33; endpoint types: /individuals/60/type; /individuals/15/type`
adenosine 5'-phosphosulfate + ATP <=> 3'-phosphoadenylyl-sulfate + ADP + H⁺ `gomodel:ADENYLYLSULFKIN-RXN`	has_input	ATP(4-) `CHEBI:30616`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:ADENYLYLSULFKIN-RXN RO:0002233 gomodel:CHEBI_30616_ADENYLYLSULFKIN-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/34; endpoint types: /individuals/0/type; /individuals/10/type`
sulfate + ATP + H⁺ <=> adenosine 5'-phosphosulfate + diphosphate `gomodel:SULFATE-ADENYLYLTRANS-RXN`	part_of	sulfate assimilation `GO:0000103`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFATE-ADENYLYLTRANS-RXN BFO:0000050 gomodel:YeastPathways_SO4ASSIM-PWY/YeastPathways_SO4ASSIM-PWY. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/35; endpoint types: /individuals/21/type; /individuals/61/type`
MET16 Scer `SGD:S000006371`	enables	adenosine 3',5'-bisphosphate + sulfite + an oxidized thioredoxin + 2 H⁺ <=> 3'-phosphoadenylyl-sulfate + a reduced thioredoxin `gomodel:1.8.4.8-RXN`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:1.8.4.8-RXN RO:0002333 gomodel:YPR167C-MONOMER_1.8.4.8-RXN_controller. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/36; endpoint types: /individuals/60/type; /individuals/25/type`
adenosine 3',5'-bisphosphate + sulfite + an oxidized thioredoxin + 2 H⁺ <=> 3'-phosphoadenylyl-sulfate + a reduced thioredoxin `gomodel:1.8.4.8-RXN`	provides_input_for	hydrogen sulfide + 3 NADP⁺ + 3 H₂O <- sulfite + 3 NADPH + 5 H⁺ `gomodel:SULFITE-REDUCT-RXN`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:1.8.4.8-RXN RO:0002413 gomodel:SULFITE-REDUCT-RXN. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/37; endpoint types: /individuals/60/type; /individuals/22/type`
hydrogen sulfide + 3 NADP⁺ + 3 H₂O <- sulfite + 3 NADPH + 5 H⁺ `gomodel:SULFITE-REDUCT-RXN`	part_of	sulfate assimilation `GO:0000103`	gomodel:YeastPathways_SO4ASSIM-PWY Source assertion: The model asserts gomodel:SULFITE-REDUCT-RXN BFO:0000050 gomodel:YeastPathways_SO4ASSIM-PWY/YeastPathways_SO4ASSIM-PWY. Source location `./YeastPathways_SO4ASSIM-PWY.json#/facts/38; endpoint types: /individuals/22/type; /individuals/61/type`
CPX-3163 Scer `SGD:S000217821`	has_part ECO:0005547: inferred by curator; source stoichiometry minValue: 2, maxValue: 2	Fe4S4 iron-sulfur cluster `CHEBI:64607`	SGD:S000217821 Source assertion: Complex Portal CPX-3163 lists CHEBI:64607 as a small molecule component; complex evidence is ECO:0005547: inferred by curator. Source location `Complex Portal JSON #/participants/2; /evidenceType`
CPX-3163 Scer `SGD:S000217821`	has_part ECO:0005547: inferred by curator; source stoichiometry minValue: 2, maxValue: 2	FAD(3-) `CHEBI:57692`	SGD:S000217821 Source assertion: Complex Portal CPX-3163 lists CHEBI:57692 as a small molecule component; complex evidence is ECO:0005547: inferred by curator. Source location `Complex Portal JSON #/participants/3; /evidenceType`
CPX-3163 Scer `SGD:S000217821`	has_part ECO:0005547: inferred by curator; source stoichiometry minValue: 2, maxValue: 2	FMN(3-) `CHEBI:58210`	SGD:S000217821 Source assertion: Complex Portal CPX-3163 lists CHEBI:58210 as a small molecule component; complex evidence is ECO:0005547: inferred by curator. Source location `Complex Portal JSON #/participants/4; /evidenceType`
CPX-3163 Scer `SGD:S000217821`	has_part ECO:0005547: inferred by curator; source stoichiometry minValue: 2, maxValue: 2	siroheme `CHEBI:28599`	SGD:S000217821 Source assertion: Complex Portal CPX-3163 lists CHEBI:28599 as a small molecule component; complex evidence is ECO:0005547: inferred by curator. Source location `Complex Portal JSON #/participants/5; /evidenceType`
CPX-3163 Scer `SGD:S000217821`	located_in Complex location from the independent composition source; original GO-CAM activity locations are retained with their own provenance.	cytoplasm `GO:0005737`	SGD:S000217821 Source assertion: Complex Portal CPX-3163 records cellular location GO:0005737 (cytoplasm); its evidence classification is ECO:0005547: inferred by curator. Source location `Complex Portal JSON #/crossReferences/1; /evidenceType`
MET3 Scer `SGD:S000003771`	located_in Physical protein location from reviewed UniProt. ECO:0000255 (HAMAP-Rule:MF_03106); ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:P08536 Source assertion: UniProtKB:P08536 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/211/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P08536 Source assertion: UniProtKB:P08536 has the exact SGD cross-reference SGD:S000003771. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/211/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
MET5 Scer `SGD:S000003898`	located_in Physical protein location from reviewed UniProt. ECO:0000269 (PubMed:14562095)	cytoplasm `GO:0005737`	UniProtKB:P47169 Source assertion: UniProtKB:P47169 annotates location Cytoplasm (SL-0086). Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1984/comments/1/subcellularLocations/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d` UniProtKB:P47169 Source assertion: UniProtKB:P47169 has the exact SGD cross-reference SGD:S000003898. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_subcellular_location%2Ccc_function%2Ccc_caution%2Corganism_id#/results/1984/uniProtKBCrossReferences/0; sha256=95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d`
MET10 Scer `SGD:S000001926`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 Binds 1 FAD per subunit. UniProt note evidence: ECO:0000250	FAD(3-) `CHEBI:57692`	UniProtKB:P39692 Source assertion: UniProtKB:P39692 annotates CHEBI:57692 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3677/comments/0/cofactors/0; /results/3677/comments/0/note` UniProtKB:P39692 Source assertion: UniProtKB:P39692 has the exact SGD cross-reference SGD:S000001926. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3677/uniProtKBCrossReferences/0`
MET10 Scer `SGD:S000001926`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 Binds 1 FMN per subunit. UniProt note evidence: ECO:0000250	FMN(3-) `CHEBI:58210`	UniProtKB:P39692 Source assertion: UniProtKB:P39692 annotates CHEBI:58210 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3677/comments/1/cofactors/0; /results/3677/comments/1/note` UniProtKB:P39692 Source assertion: UniProtKB:P39692 has the exact SGD cross-reference SGD:S000001926. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/3677/uniProtKBCrossReferences/0`
MET5 Scer `SGD:S000003898`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 Binds 1 siroheme per subunit. UniProt note evidence: ECO:0000250	siroheme(8-) `CHEBI:60052`	UniProtKB:P47169 Source assertion: UniProtKB:P47169 annotates CHEBI:60052 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1984/comments/0/cofactors/0; /results/1984/comments/0/note` UniProtKB:P47169 Source assertion: UniProtKB:P47169 has the exact SGD cross-reference SGD:S000003898. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1984/uniProtKBCrossReferences/0`
MET5 Scer `SGD:S000003898`	has_cofactor UniProtKB reviewed (Swiss-Prot) annotation. UniProt evidence: ECO:0000250 Binds 1 [4Fe-4S] cluster per subunit. UniProt note evidence: ECO:0000250	tetra-mu3-sulfido-tetrairon `CHEBI:49883`	UniProtKB:P47169 Source assertion: UniProtKB:P47169 annotates CHEBI:49883 as a cofactor of this protein. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1984/comments/1/cofactors/0; /results/1984/comments/1/note` UniProtKB:P47169 Source assertion: UniProtKB:P47169 has the exact SGD cross-reference SGD:S000003898. Source location `https://rest.uniprot.org/uniprotkb/stream?query=taxonomy_id%3A559292+AND+reviewed%3Atrue&format=json&fields=accession%2Cprotein_name%2Cxref_sgd%2Ccc_cofactor%2Corganism_id%2Ccc_caution [sha256:1378282d22c5518215707964664b55946f468ba74a227a6ea1bbf27db69ddbc4] #/results/1984/uniProtKBCrossReferences/0`

References

gomodel:YeastPathways_SO4ASSIM-PWY SGD GO-CAM assimilatory sulfate reduction I - imported from: Saccharomyces Genome Database (source version: Pinned Noctua JSON archive; model date 2026-07-15; member ./YeastPathways_SO4ASSIM-PWY.json)
SGD:S000217821 Complex Portal CPX-3163: Sulfite reductase complex (NADPH) (source version: Inspected API snapshot 2026-10-05; release dates 2014-10-16)
UniProtKB:P39692 Reviewed UniProtKB P39692 SGD cross-reference (source version: UniProt release 2026_03)
UniProtKB:P47169 Reviewed UniProtKB P47169 SGD cross-reference (source version: UniProt release 2026_03)
UniProtKB:P08536 Reviewed UniProtKB P08536 location annotations (source version: UniProt release 2026_03)

Curation history

2026-10-05T06:05:08Z Codex; review-causal-graph; Reviewed every native GO-CAM fact; preserved precise input/output, causal, location, membership and complex-part relations with traceable structured source evidence. Kept distinct generic physical instances and recorded source-scope exclusions in the cohort review ledger. Reviewed exact Complex Portal composition, source evidence grades, and independent physical complex locations.
2026-10-05T06:17:04Z Codex; review-complex-composition; Reviewed exact Complex Portal composition, source evidence grades, and independent physical complex locations; reused existing proteins through reviewed UniProt SGD cross-references.
2026-10-05T06:43:56Z Codex
- EDIT — Audit causal activity compartments and preserve scoped protein location evidence
  Reviewed all 4 activity location assignments and 5 mapped physical proteins against reviewed S288C UniProt release2026_03 location/function/caution batch SHA256 95f72dcc644358c480e0e59f996cf88533558b5fa4256460a030e0a1eaf9678d. Added 2 independently supported location edges and quarantined 0 generic cytosol source assertions. Mapped physical protein locations via exact SGD cross-references; retained source ECO grades, molecular form and conditional notes. Physical protein location alone never generates activity location. Explicitly inspected functional assertions support bounded activity projections. Every original activity location and exclusion is recorded in reports/causal_graph_review/gocam-location-review.json. No independent location annotation is treated as an evidence gap, not a negative result. Cytoplasm is not narrowed to cytosol. Microsome fractions are not projected as in-vivo organelles. Semantic validation passed all85 records;4 independent source-role acceptance tests passed.
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