Entodiniomorphid subkinetal myoneme system

cellstructuremech:entodiniomorphid_subkinetal_myoneme_system · Cytoskeleton · Other · Proposed

A system of calmyonemin-containing contractile filament bundles associated with the bases of retractile apical ciliature in entodiniomorphid ciliates, characterized in Eudiplodinium maggii and Entodinium bursa. This record denotes the subkinetal bundles, not all other calmyonemin-positive arrays or the cilia themselves. [DOI:10.1016/S0248-4900(94)80014-6]

Synonyms: subkinetal myonemes

Part of: GO:0005856 — cytoskeleton (no local record)

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Stoichiometry	Essential	Role
Calmyonemin proteins	Protein	part of	Reviewed label only (reviewed name; no accession asserted)			UNKNOWN	Antibody-localized myonemal constituents; molecular identity and native inventory are not resolved to specific paralogues. `DOI:10.1002/cm.970270208` Primary abstract reports A69 immunoelectron localization to E. maggii myonemes plus biochemical calcium-binding and centrin-similarity evidence. An essential role in retraction is postulated, not experimentally established. `DOI:10.1016/S0248-4900(94)80014-6` Publisher-deposited abstract reports anti-calmyonemin immunofluorescence at E. bursa ciliature bases and describes subkinetal myoneme assembly. Other antibody-positive arrays are not merged into this structure.

Canonical examples

NCBITaxon:47892 Eudiplodinium maggii — Species used for myonemal protein characterization and immunoelectron localization, as reported in the primary abstract. Identifier verified at ENA. [DOI:10.1002/cm.970270208]
NCBITaxon:358014 Entodinium bursa — Species used for calmyonemin immunofluorescence and cell-cycle observations. Abstract-level example; identifier verified at ENA. No clade-wide prevalence is inferred. [DOI:10.1016/S0248-4900(94)80014-6]

Functions

Apical ciliature retraction — The subkinetal contractile bundles support withdrawal of apical ciliature during stress-associated retraction; the molecular force-generating mechanism remains unresolved here.
- DOI:10.1002/cm.970270208 Abstract places converging myonemes beneath retractile apical ciliary zones.
- DOI:10.1016/S0248-4900(94)80014-6 Abstract explicitly describes subkinetal myonemes as contractile bundles powering ciliature retraction.

Discussions

CURATION_TODO (RESOLVED): Delimit one subkinetal bundle system rather than a generic myoneme or a record per species. Shared calcium-binding proteins do not make all ciliate contractile architectures the same structure.
CURATION_TODO (OPEN): Resolve the native calmyonemin sequences, exact family grounding and other bundle constituents. Antibody cross-reactivity and EF-hand similarity do not establish an exact taxon-neutral family mapping or complete composition.
CURATION_TODO (RESOLVED): Keep other calmyonemin-positive arrays and their cell-cycle behavior outside this record. A shared immunoreactive protein does not establish anatomical identity or a shared assembly pathway.
CURATION_TODO (OPEN): Inspect full Methods and microscopy panels, then identify selective tests of assembly and retraction requirements. Readable primary abstracts support this bounded anatomical record but not a resolved molecular contraction cycle.

Evidence

DOI:10.1002/cm.970270208 David and Vigues (1994), PMID 8162623. PubMed and publisher-deposited Crossref abstracts read. Publisher PDF returned HTTP 403; full Methods and figures were not inspected.
DOI:10.1016/S0248-4900(94)80014-6 Vigues and David (1994 print; 2012 online date in Crossref). DOI, authors and publisher-deposited abstract verified at Crossref. Publisher PDF returned HTTP 403; full Methods and figures were not inspected. A quoted Europe PMC DOI lookup returned no hit, not proof that no PubMed record exists.
GO:0005622 Verified non-obsolete intracellular anatomical structure: broad is-a parent only.
GO:0005856 — cytoskeleton Verified non-obsolete cytoskeleton: encompassing framework, used as part_of.

Curation history

2026-10-04T02:31:37Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-10-04T02:33:01Z codex · EXPANDED_RECORD — Added primary-abstract-backed calmyonemin class, verified species and hierarchy, retraction function, and explicit source-access, composition, causal and other-array boundaries.

Source: data/structures/cytoskeleton/entodiniomorphid_subkinetal_myoneme_system.yaml