penta-snRNP complex

GO:0071003 · Ribonucleoprotein · Ribonucleoprotein complex · Proposed

A ribonucleoprotein complex that is formed by the association of the U1, U2, U4/U6 and U5 small nuclear ribonucleoproteins. [GO:0071003]

Synonyms: penta-RNP complex

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Role
U1 snRNP	Protein complex	part of	`GO:0005685`	U1 small nuclear ribonucleoprotein subcomplex that associates with U2, U4/U6 and U5 snRNPs in the penta-snRNP complex. `GO:0071003` GO:0071003 defines the penta-snRNP complex as formed by association of the U1, U2, U4/U6 and U5 snRNPs. `GO:0005685` GO:0005685 is the Gene Ontology cellular-component term for the U1 snRNP.
U2 snRNP	Protein complex	part of	`GO:0005686`	U2 small nuclear ribonucleoprotein subcomplex that associates with U1, U4/U6 and U5 snRNPs in the penta-snRNP complex. `GO:0071003` GO:0071003 defines the penta-snRNP complex as formed by association of the U1, U2, U4/U6 and U5 snRNPs. `GO:0005686` GO:0005686 is the Gene Ontology cellular-component term for the U2 snRNP.
U4/U6 snRNP	Protein complex	part of	`GO:0071001`	U4/U6 small nuclear ribonucleoprotein subcomplex that associates with U1, U2 and U5 snRNPs in the penta-snRNP complex. `GO:0071003` GO:0071003 defines the penta-snRNP complex as formed by association of the U1, U2, U4/U6 and U5 snRNPs. `GO:0071001` GO:0071001 is the Gene Ontology cellular-component term for the U4/U6 snRNP.
U5 snRNP	Protein complex	part of	`GO:0005682`	U5 small nuclear ribonucleoprotein subcomplex that associates with U1, U2 and U4/U6 snRNPs in the penta-snRNP complex. `GO:0071003` GO:0071003 defines the penta-snRNP complex as formed by association of the U1, U2, U4/U6 and U5 snRNPs. `GO:0005682` GO:0005682 is the Gene Ontology cellular-component term for the U5 snRNP.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: The penta-snRNP complex was biochemically characterized in budding yeast; broader eukaryotic distribution is left for follow-up curation. [DOI:10.1016/s1097-2765(02)00436-7]
NCBITaxon:9606 Homo sapiens — COMMON: A preassembled penta-snRNP containing five spliceosomal snRNPs was detected in HeLa nuclear extracts; broader mammalian distribution is left for follow-up curation. [DOI:10.1128/MCB.23.10.3442-3455.2003]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Budding-yeast penta-snRNP characterized by Stevens, Ryan, Ge and colleagues in a composition and functional study. [DOI:10.1016/s1097-2765(02)00436-7]
NCBITaxon:9606 Homo sapiens — HeLa nuclear extracts in which Malca, Shomron and Ast detected a five-spliceosomal-snRNP penta-snRNP complex. [DOI:10.1128/MCB.23.10.3442-3455.2003]

Mechanism graphs

U1, U2, U4/U6 and U5 snRNPs form the penta-snRNP (ASSEMBLY)

The penta-snRNP complex forms by association of U1, U2, U4/U6 and U5 small nuclear ribonucleoproteins.

Subject	Predicate	Object	Evidence
U1 snRNP	contributes to assembly of	penta-snRNP complex	`GO:0071003` GO:0071003 includes U1 among the associating snRNPs that form the penta-snRNP complex.
U2 snRNP	contributes to assembly of	penta-snRNP complex	`GO:0071003` GO:0071003 includes U2 among the associating snRNPs that form the penta-snRNP complex.
U4/U6 snRNP	contributes to assembly of	penta-snRNP complex	`GO:0071003` GO:0071003 includes U4/U6 among the associating snRNPs that form the penta-snRNP complex.
U5 snRNP	contributes to assembly of	penta-snRNP complex	`GO:0071003` GO:0071003 includes U5 among the associating snRNPs that form the penta-snRNP complex.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral protein and RNA boundaries for yeast and human penta-snRNP complexes. The first GO:0071003 record captures the GO-grounded U1, U2, U4/U6 and U5 snRNP subcomplexes that define the penta-snRNP. Individual spliceosomal RNA and protein-family boundaries should be resolved before splitting this inventory.

Evidence

GO:0071003 Gene Ontology cellular-component term for penta-snRNP complex and its exact penta-RNP complex synonym.
GO:0097525 Gene Ontology cellular-component parent term for spliceosomal snRNP complex.
GO:0005685 Gene Ontology cellular-component term for the U1 snRNP.
GO:0005686 Gene Ontology cellular-component term for the U2 snRNP.
GO:0071001 Gene Ontology cellular-component term for the U4/U6 snRNP.
GO:0005682 Gene Ontology cellular-component term for the U5 snRNP.
DOI:10.1016/s1097-2765(02)00436-7 Stevens, Ryan, Ge and colleagues 2002, Composition and functional characterization of the yeast spliceosomal penta-snRNP, Molecular Cell.
PMID:11804584 PubMed identifier for Stevens, Ryan, Ge and colleagues 2002 as reported by PubMed.
DOI:10.1128/MCB.23.10.3442-3455.2003 Malca, Shomron and Ast 2003, The U1 snRNP base pairs with the 5' splice site within a penta-snRNP complex, Molecular and Cellular Biology.
PMID:12724403 PubMed identifier for Malca, Shomron and Ast 2003 as reported by PubMed.

Curation history

2026-09-25T17:57:18Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-25T17:58:19Z codex · EXPANDED_RECORD — Added the GO exact synonym, spliceosomal snRNP parentage, GO-grounded U1, U2, U4/U6 and U5 snRNP components, yeast and human penta-snRNP evidence, a nonmechanistic assembly graph and a component-boundary TODO.

Source: data/structures/ribonucleoprotein/penta_snrnp_complex.yaml