modified_artificial_seawater_medium

CultureMech:000178 algae COMPLEX LIQUID
Original name: Modified Artificial Seawater Medium

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Synonyms
  • {'name': 'dyv_medium', 'source': 'unknown', 'original_category': 'algae'}

Composition

flowchart LR
    CultureMech_000178["modified_artificial_seawater_medium"]
    ing0_1["1"]
    CultureMech_000178 --> ing0_1
    ing1_2["2"]
    CultureMech_000178 --> ing1_2
    ing2_3["3"]
    CultureMech_000178 --> ing2_3
    ing3_4["4"]
    CultureMech_000178 --> ing3_4
    ing4_5["5"]
    CultureMech_000178 --> ing4_5
    ing5_6["6"]
    CultureMech_000178 --> ing5_6
    ing6_7["7"]
    CultureMech_000178 --> ing6_7
    ing7_8["8"]
    CultureMech_000178 --> ing7_8
    ing8_9["9"]
    CultureMech_000178 --> ing8_9
    ing9_10["10"]
    CultureMech_000178 --> ing9_10
    ing10_11["11"]
    CultureMech_000178 --> ing10_11
    ing11_12["12"]
    CultureMech_000178 --> ing11_12
    ing12_13["13"]
    CultureMech_000178 --> ing12_13

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 1 variable G_PER_L Original amount: MES(CAS: 7365-45-9)
2 2 variable G_PER_L Original amount: MWC Metal Solution
3 3 variable G_PER_L Original amount: CaCl2•2H2O(CAS: 10035-04-8)
4 4 variable G_PER_L Original amount: MgSO4•7H2O(CAS: 10034-99-8)
5 5 variable G_PER_L Original amount: Na2Glycerophosphate•5H2O(CAS: 13408-09-8)
6 6 variable G_PER_L Original amount: NaNO3(CAS: 7631-99-4)
7 7 variable G_PER_L Original amount: Na2SiO3•9H2O(CAS: 13517-24-3)
8 8 variable G_PER_L Original amount: NH4NO3(CAS: 6484-52-2)
9 9 variable G_PER_L Original amount: KCl(CAS: 7447-40-7)
10 10 variable G_PER_L Original amount: DYV Metal Solution
11 11 variable G_PER_L Original amount: Vitamin B12
12 12 variable G_PER_L Original amount: Biotin Vitamin Solution
13 13 variable G_PER_L Original amount: Thiamine Vitamin Solution

Preparation

  1. Important Notes To Consider: The quality of water, including natural seawater, is important.
  2. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc.
  3. Natural seawater and natural freshwater should be obtained from a non-polluted source. When dissolving chemicals, wait for the first component to dissolve before adding the second.
  4. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently.
  5. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts. Attention should be given to the pH of the final medium.
  6. In most cases, if pH adjustment is required, this occurs before sterilization.
  7. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers.
  8. This would make the medium very alkaline soon after removal from the autoclave.
  9. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: modified-artificial-seawater-medium, URL: https://utex.org/products/modified-artificial-seawater-medium
2026-03-13T06:18:53.438935Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000178
2026-03-17T19:45:04.372745+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Artificial Seawater Medium" to "modified_artificial_seawater_medium" for consistent matching
2026-05-16T09:02:56.036821+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.242536+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-06T03:04:19.526219+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: DYV_Medium, Modified_Artificial_Seawater_Medium
2026-08-06T03:04:19.526258+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 7317a63a1c1ccff3c0c8fcaa392dd9d23ad81a52174fe9de69218da7776e0e21