wc_medium

CultureMech:000240 algae COMPLEX LIQUID
Original name: WC Medium

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Synonyms
  • {'name': 'artificial_seawater_medium', 'source': 'unknown', 'original_category': 'algae'}
  • {'name': 'ldm_medium', 'source': 'unknown', 'original_category': 'algae'}
  • {'name': 'modified_bolds_3n_medium', 'source': 'unknown', 'original_category': 'algae'}
  • {'name': 'n_20_medium', 'source': 'unknown', 'original_category': 'algae'}

Composition

flowchart LR
    CultureMech_000240["wc_medium"]
    ing0_1["1"]
    CultureMech_000240 --> ing0_1
    ing1_2["2"]
    CultureMech_000240 --> ing1_2
    ing2_3["3"]
    CultureMech_000240 --> ing2_3
    ing3_4["4"]
    CultureMech_000240 --> ing3_4
    ing4_5["5"]
    CultureMech_000240 --> ing4_5
    ing5_6["6"]
    CultureMech_000240 --> ing5_6
    ing6_7["7"]
    CultureMech_000240 --> ing6_7
    ing7_8["8"]
    CultureMech_000240 --> ing7_8
    ing8_9["9"]
    CultureMech_000240 --> ing8_9
    ing9_10["10"]
    CultureMech_000240 --> ing9_10
    ing10_11["11"]
    CultureMech_000240 --> ing10_11

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 1 variable G_PER_L Original amount: NaCl(Fisher S271-500)
2 2 variable G_PER_L Original amount: MgSO4•7H2O(Sigma 230391)
3 3 variable G_PER_L Original amount: KCl(Fisher P 217)
4 4 variable G_PER_L Original amount: NaNO3(Fisher BP360-500)
5 5 variable G_PER_L Original amount: CaCl2•2H2O(Sigma C-3881)
6 6 variable G_PER_L Original amount: KH2PO4(Sigma P 0662)
7 7 variable G_PER_L Original amount: Tricine (adjust to pH 8)(Sigma T-5816-256)
8 8 variable G_PER_L Original amount: P-II Metal Solution
9 9 variable G_PER_L Original amount: Chelated Iron Solution
10 10 variable G_PER_L Original amount: NH4Cl(Fisher A 649-500)
11 11 variable G_PER_L Original amount: Vitamin B12

Preparation

  1. Important Notes To Consider: The quality of water, including natural seawater, is important.
  2. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc.
  3. Natural seawater and natural freshwater should be obtained from a non-polluted source. When dissolving chemicals, wait for the first component to dissolve before adding the second.
  4. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently.
  5. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts. Attention should be given to the pH of the final medium.
  6. In most cases, if pH adjustment is required, this occurs before sterilization.
  7. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers.
  8. This would make the medium very alkaline soon after removal from the autoclave.
  9. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.

Target organisms

No target organisms are recorded.

Provenance

Merged from 5 source records.

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: wc-medium, URL: https://utex.org/products/wc-medium
2026-03-13T06:18:53.698627Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000240
2026-03-17T19:45:04.602428+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "WC Medium" to "wc_medium" for consistent matching
2026-05-16T09:02:56.174028+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.394992+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-06T03:04:19.361793+00:00 recipe-merger Merged 5 duplicate recipes into canonical record Sources: Artificial_Seawater_Medium, LDM_Medium, Modified_Bolds_3N_Medium, N_20_Medium, WC_Medium
2026-08-06T03:04:19.361849+00:00 merge_recipes.py MERGED_RECIPES merged 5 source recipe(s) on fingerprint 45f7523e48d1b708d46cf2b78b00715ccc525331a6a76c7e14ba1a90455d196d