methanomassiliicoccus_medium

Overview

External ontology

mediadive.medium:119b (source link unavailable) — METHANOMASSILIICOCCUS MEDIUM

Applications

• Microbial cultivation

Synonyms

• {'name': 'methanobacterium_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:119', 'original_category': 'archaea'}
• {'name': 'for_dsm_16632_and_dsm_16643', 'source': 'KOMODO', 'source_id': 'komodo.medium:119.1', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_11975', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_11975', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_11977', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_11977', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_11978', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_11978', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_11979', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_11979', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_11995', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_11995', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_1224', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_1224', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_15163', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_15163', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_3468', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_3468', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_4855', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_4855', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_6216', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_6216', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_7057', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_7057', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_7256', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_7256', 'original_category': 'bacterial'}
• {'name': 'medium_119_modified_for_dsm_9575', 'source': 'KOMODO', 'source_id': 'komodo.medium:119_9575', 'original_category': 'bacterial'}

pH

7.6

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119b.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_000645["methanomassiliicoccus_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000645 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000645 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_000645 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000645 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000645 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000645 --> ing5_Yeast_extract
    ing6_Na_acetate["Na-acetate"]
    CultureMech_000645 --> ing6_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Na_acetate -.-> CHEBI_32954
    ing7_Na_formate["Na-formate"]
    CultureMech_000645 --> ing7_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing7_Na_formate -.-> CHEBI_62965
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000645 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_Methanol["Methanol"]
    CultureMech_000645 --> ing9_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing9_Methanol -.-> CHEBI_17790
    ing10_NaHCO3["NaHCO3"]
    CultureMech_000645 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000645 --> ing11_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000645 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209
    ing13_HCl["HCl"]
    CultureMech_000645 --> ing13_HCl
    CHEBI_17883["CHEBI:17883"]
    ing13_HCl -.-> CHEBI_17883
    ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000645 --> ing14_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing15_ZnCl2["ZnCl2"]
    CultureMech_000645 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000645 --> ing16_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing17_H3BO3["H3BO3"]
    CultureMech_000645 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000645 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000645 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000645 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000645 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_NaOH["NaOH"]
    CultureMech_000645 --> ing22_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing22_NaOH -.-> CHEBI_32145
    ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000645 --> ing23_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000645 --> ing24_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing25_Sludge["Sludge"]
    CultureMech_000645 --> ing25_Sludge
    ing26_Isobutyric_acid["Isobutyric acid"]
    CultureMech_000645 --> ing26_Isobutyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing26_Isobutyric_acid -.-> CHEBI_16135
    ing27_Tryptone["Tryptone"]
    CultureMech_000645 --> ing27_Tryptone
    ing28_Yeast_extract["Yeast extract"]
    CultureMech_000645 --> ing28_Yeast_extract
    ing29_Sodium_chloride["Sodium chloride"]
    CultureMech_000645 --> ing29_Sodium_chloride
    ing29_Sodium_chloride -.-> CHEBI_26710
    CultureMech_000645 --> more["...4 more"]

Ingredients (34)

Preparation

1. Dissolve ingredients except methanol, bicarbonate, cysteine and sulfide. Adjust pH of medium to 7.2 and sparge with 100% N2 gas for 30 - 45 min to make it anoxic. Then dispense medium under same gas atmosphere into anoxic Hungate-type tubes to 30% v/v of their volume and autoclave. Add methanol (from 15% v/v stock solution), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use check pH of complete medium and adjust to 7.6, if necessary.
2. After inoculation, add sterile 80% H2 and 20% CO2 gas mixture to 0.5 bar overpressure. After growth becomes visible overpressure of 80% H2 and 20% CO2 gas mixture can be increased to 1 bar.
3. Note: Use 10% (v/v) as inoculum.
4. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
5. Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37°C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at 8-12°C in the dark.
6. Adjust pH to 7.5 with concentrated NaOH.
7. The ferrous sulfate solution is not stable and should be freshly prepared.

Target organisms

No target organisms are recorded.

Provenance

Merged from 16 source records.

• KOMODO_119_METHANOBACTERIUM_medium (CultureMech:003939)
• methanomassiliicoccus_medium (CultureMech:000645)
• for_dsm_16632_and_dsm_16643 (CultureMech:003918)
• medium_119_modified_for_dsm_11975 (CultureMech:003911)
• medium_119_modified_for_dsm_11977 (CultureMech:003912)
• medium_119_modified_for_dsm_11978 (CultureMech:003913)
• medium_119_modified_for_dsm_11979 (CultureMech:003914)
• medium_119_modified_for_dsm_11995 (CultureMech:003915)
• medium_119_modified_for_dsm_1224 (CultureMech:003916)
• medium_119_modified_for_dsm_15163 (CultureMech:003917)
• medium_119_modified_for_dsm_3468 (CultureMech:003919)
• medium_119_modified_for_dsm_4855 (CultureMech:003920)
• medium_119_modified_for_dsm_6216 (CultureMech:003921)
• medium_119_modified_for_dsm_7057 (CultureMech:003922)
• medium_119_modified_for_dsm_7256 (CultureMech:003923)
• medium_119_modified_for_dsm_9575 (CultureMech:003924)