Overview
- External ontology
- mediadive.medium:1291 (source link unavailable) — CHTHONOMONAS MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 't49_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:1291', 'original_category': 'bacterial'}
- pH range
- {'min': 5.0, 'max': 5.3}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1291.pdf
Composition
flowchart LR
CultureMech_000754["chthonomonas_medium"]
ing0_Gellan_Gum["Gellan Gum"]
CultureMech_000754 --> ing0_Gellan_Gum
CHEBI_85248["CHEBI:85248"]
ing0_Gellan_Gum -.-> CHEBI_85248
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_000754 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_K2HPO4["K2HPO4"]
CultureMech_000754 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000754 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000754 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_Yeast_extract["Yeast extract"]
CultureMech_000754 --> ing5_Yeast_extract
ing6_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_000754 --> ing6_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing6_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_000754 --> ing7_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
ing8_ZnCl2["ZnCl2"]
CultureMech_000754 --> ing8_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing8_ZnCl2 -.-> CHEBI_49976
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000754 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_H3BO3["H3BO3"]
CultureMech_000754 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000754 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000754 --> ing12_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000754 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000754 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_Vitamin_B12["Vitamin B12"]
CultureMech_000754 --> ing15_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing15_Vitamin_B12 -.-> CHEBI_176843
ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000754 --> ing16_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
ing17_D_____biotin["D-(+)-biotin"]
CultureMech_000754 --> ing17_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing17_D_____biotin -.-> CHEBI_15956
ing18_Nicotinic_acid["Nicotinic acid"]
CultureMech_000754 --> ing18_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing18_Nicotinic_acid -.-> CHEBI_15940
ing19_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_000754 --> ing19_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing19_Calcium_pantothenate -.-> CHEBI_31345
ing20_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000754 --> ing20_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing20_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing21_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_000754 --> ing21_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing21_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Gellan Gum | 0.5 G_PER_L |
CHEBI:85248
gellan gum
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
4 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.45 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 | Yeast extract | 0.01 G_PER_L | ||
| 7 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
5.2 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 8 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 9 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 17 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 18 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 19 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 20 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 21 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.3 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 22 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.2 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Preparation
- Add gellan gum and boil to dissolve. Then add the remaining ingredients (except magnesium chloride, yeast extract and vitamins), adjust the pH value to 5.0 to 5.3 and fill the medium under an air atmosphere into tubes of the Hungate type or similar vials up to 30% their volume. After autoclaving add magnesium chloride, yeast extract and vitamins from steril stock solutions. Vitamins should be sterilized by filtration.
- Note: Incubation with shaking may improve cell yield.
- Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- chthonomonas_medium (CultureMech:000754)
- t49_medium (CultureMech:004041)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.009662Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1291 |
| 2026-03-13T06:18:58.095631Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000754 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.454134+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CHTHONOMONAS MEDIUM" to "chthonomonas_medium" for consistent matching |
| 2026-05-20T06:01:11.637055+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '5-5.3' -> {'min': 5.0, 'max': 5.3} |
| 2026-06-05T07:40:53.634016+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.591323+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:27.741301+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: chthonomonas_medium, t49_medium |
| 2026-08-06T03:05:27.741336+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 5a3ad768e96cd830185a4cd6b4b8bc592585bb661e08adb1efc8e0664240fb87 |