methanocorpusculum_aggregans_medium

CultureMech:001423 archaea COMPLEX LIQUID
Original name: METHANOCORPUSCULUM AGGREGANS MEDIUM

Overview

External ontology
mediadive.medium:321 (source link unavailable) — METHANOCORPUSCULUM AGGREGANS MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'methanogenium_aggregans_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:321', 'original_category': 'archaea'}
  • {'name': 'methanogenium_aggregans_medium_replace_sludge_fluid_medium_119_with_rumen_fluid_clarified', 'source': 'KOMODO', 'source_id': 'komodo.medium:321_replace_Sludge fluid (medium 119)_with_Rumen fluid, clarified', 'original_category': 'archaea'}
pH range
{'min': 6.8, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium321.pdf

Composition

flowchart LR
    CultureMech_001423["methanocorpusculum_aggregans_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_001423 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_001423 --> ing1_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001423 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_Na_formate["Na-formate"]
    CultureMech_001423 --> ing3_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing3_Na_formate -.-> CHEBI_62965
    ing4_Na_acetate["Na-acetate"]
    CultureMech_001423 --> ing4_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Na_acetate -.-> CHEBI_32954
    ing5_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001423 --> ing5_Trypticase_peptone
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001423 --> ing6_Yeast_extract
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001423 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_Na2CO3["Na2CO3"]
    CultureMech_001423 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001423 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001423 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_KH2PO4["KH2PO4"]
    CultureMech_001423 --> ing11_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing11_KH2PO4 -.-> CHEBI_63036
    ing12__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001423 --> ing12__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing12__NH4_2SO4 -.-> CHEBI_62946
    ing13_NaCl["NaCl"]
    CultureMech_001423 --> ing13_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing13_NaCl -.-> CHEBI_26710
    ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001423 --> ing14_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001423 --> ing15_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001423 --> ing16_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing17_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001423 --> ing17_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing17_MnSO4_x_H2O -.-> CHEBI_86364
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001423 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001423 --> ing19_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001423 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001423 --> ing21_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing22_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001423 --> ing22_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing22_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing23_H3BO3["H3BO3"]
    CultureMech_001423 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001423 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001423 --> ing25_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing26_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001423 --> ing26_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing26_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001423 --> ing27_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing28_Sludge["Sludge"]
    CultureMech_001423 --> ing28_Sludge

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
2 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
0.4 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.4 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 Na-formate
CHO2.Na 68.007 g/mol
5 G_PER_L CHEBI:62965
sodium formate
5 Na-acetate
C2H3O2.Na 82.034 g/mol
1 G_PER_L CHEBI:32954
sodium acetate
6 Trypticase peptone 1 G_PER_L
7 Yeast extract 5.0 G_PER_L [Merged 2 duplicates: 1.0, 4.0]
8 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
9 Na2CO3
CO3.2Na 105.988 g/mol
1.5 G_PER_L CHEBI:29377
sodium carbonate
10 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.2 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
11 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.2 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
12 KH2PO4
H2O4P.K 136.084 g/mol
6 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
13 (NH4)2SO4
2H4N.O4S 132.141 g/mol
6 G_PER_L CHEBI:62946
ammonium sulfate
14 NaCl
Cl.Na 58.443 g/mol
13.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 12.0, 1.0]
15 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
5.6 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 2.6, 3.0]
16 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.26 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.16, 0.1]
17 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
18 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
19 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
20 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
21 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
22 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
23 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
24 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
25 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
26 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
27 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
28 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
29 Sludge 1000 G_PER_L

Preparation

  1. Dissolve ingredients except carbonate, cysteine and sulfide, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary.
  2. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.
  3. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
  4. Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.
  5. Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37°C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at 8-12°C in the dark.

Target organisms

No target organisms are recorded.

Provenance

Merged from 3 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:10.786858Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 321
2026-03-13T03:09:41.221381Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:04.012814Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001423
2026-03-17T06:30:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.995466+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOCORPUSCULUM AGGREGANS MEDIUM" to "methanocorpusculum_aggregans_medium" for consistent matching
2026-05-20T06:01:55.518076+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:41:31.321214+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.897437+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 8 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:30.250377+00:00 recipe-merger Merged 3 duplicate recipes into canonical record Sources: methanocorpusculum_aggregans_medium, methanogenium_aggregans_medium, methanogenium_aggregans_medium_replace_sludge_fluid_medium_119_with_rumen_fluid_clarified
2026-08-06T03:04:30.250445+00:00 merge_recipes.py MERGED_RECIPES merged 3 source recipe(s) on fingerprint 6dc36e8a4974277e2a402228f6dd70291496ff69af21db87bb168f3a98bf1968