Overview
- External ontology
- mediadive.medium:389 (source link unavailable) — ANNWOODIA MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'thiobacillus_aquaesulis_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:389', 'original_category': 'bacterial'}
- pH
- 7.6
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium389.pdf
Composition
flowchart LR
CultureMech_001495["annwoodia_medium"]
ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_001495 --> ing0_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing1_KH2PO4["KH2PO4"]
CultureMech_001495 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_001495 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001495 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Agar["Agar"]
CultureMech_001495 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_001495 --> ing5_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing6_Na2_EDTA["Na2-EDTA"]
CultureMech_001495 --> ing6_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing6_Na2_EDTA -.-> CHEBI_64734
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001495 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001495 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001495 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001495 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_001495 --> ing11__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing11__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001495 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001495 --> ing13_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
ing14_NaOH["NaOH"]
CultureMech_001495 --> ing14_NaOH
CHEBI_32145["CHEBI:32145"]
ing14_NaOH -.-> CHEBI_32145
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
79 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
15 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.45977 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.114943 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 | Agar | 17.2414 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 6 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
250 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 7 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
50 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
11 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
7.34 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
2.5 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.5 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.5 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.2 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 15 |
NaOH
HO.Na 39.997 g/mol
|
11 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Preparation
- Autoclave solutions A, B and C and filter sterilize solution D. To complete the medium combine sterile solutions when cool.
- Adjust to pH 7.6.
- Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- annwoodia_medium (CultureMech:001495)
- thiobacillus_aquaesulis_medium (CultureMech:005166)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.005432Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 389 |
| 2026-03-13T06:19:04.677382Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001495 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.727832+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ANNWOODIA MEDIUM" to "annwoodia_medium" for consistent matching |
| 2026-06-05T07:40:49.217822+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.355525+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:23.687425+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: annwoodia_medium, thiobacillus_aquaesulis_medium |
| 2026-08-06T03:05:23.687473+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 52fd6c7188efe21405f6eaa5794e1e5ab583cfd67a0ee621c64c94b26c9fc58c |