Overview
- External ontology
- mediadive.medium:498 (source link unavailable) — PSEUDOTHERMOTOGA MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'thermotoga_ii_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:498', 'original_category': 'bacterial'}
- pH range
- {'min': 7.0, 'max': 7.2}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium498.pdf
Composition
flowchart LR
CultureMech_001625["pseudothermotoga_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001625 --> ing0_Yeast_extract
ing1_NaCl["NaCl"]
CultureMech_001625 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Na2_EDTA["Na2-EDTA"]
CultureMech_001625 --> ing2_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing2_Na2_EDTA -.-> CHEBI_64734
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001625 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001625 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001625 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_KCl["KCl"]
CultureMech_001625 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_NaBr["NaBr"]
CultureMech_001625 --> ing7_NaBr
CHEBI_63004["CHEBI:63004"]
ing7_NaBr -.-> CHEBI_63004
ing8_H3BO3["H3BO3"]
CultureMech_001625 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9__NH4_2Ni_SO4_2["(NH4)2Ni(SO4)2"]
CultureMech_001625 --> ing9__NH4_2Ni_SO4_2
CHEBI_86149["CHEBI:86149"]
ing9__NH4_2Ni_SO4_2 -.-> CHEBI_86149
ing10_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_001625 --> ing10_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing10_SrCl2_x_6_H2O -.-> CHEBI_36385
ing11_KI["KI"]
CultureMech_001625 --> ing11_KI
CHEBI_8346["CHEBI:8346"]
ing11_KI -.-> CHEBI_8346
ing12_Sodium_resazurin["Sodium resazurin"]
CultureMech_001625 --> ing12_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing12_Sodium_resazurin -.-> CHEBI_8806
ing13_KH2PO4["KH2PO4"]
CultureMech_001625 --> ing13_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing13_KH2PO4 -.-> CHEBI_63036
ing14_Starch["Starch"]
CultureMech_001625 --> ing14_Starch
CHEBI_28017["CHEBI:28017"]
ing14_Starch -.-> CHEBI_28017
ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001625 --> ing15_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing15_Na2S_x_9_H2O -.-> CHEBI_76209
ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001625 --> ing16_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
ing17_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001625 --> ing17_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing17_MnSO4_x_H2O -.-> CHEBI_86364
ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001625 --> ing18_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001625 --> ing19_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001625 --> ing20_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001625 --> ing21_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
ing22_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001625 --> ing22_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing22_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001625 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001625 --> ing24_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001625 --> ing25_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001625 --> ing26_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.488281 G_PER_L | ||
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
4.378909999999999 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 3.37891, 1.0] |
| 3 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.751953 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.859375 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.859375, 3.0] |
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.673828 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.236719 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.136719, 0.1] |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.078125 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 |
NaBr
Br.Na 102.894 g/mol
|
0.012207 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.01390625 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.00390625, 0.01] |
| 10 |
(NH4)2Ni(SO4)2
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
0.00292969 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 11 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.00185547 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 12 |
KI
I.K 166.002 g/mol
|
9.76562e-06 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 13 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000488281 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 14 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.488281 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 15 | Starch | 4.88281 G_PER_L |
CHEBI:28017
starch
|
|
| 16 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.488281 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 17 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 18 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 19 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 20 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 21 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 22 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 23 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 25 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 26 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 27 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients (except hydrogenphosphate, starch and sulfide) and adjust pH to 7.0. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% volume and autoclave. Add hydrogenphosphate, starch and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Check pH of the complete medium and adjust to 7.0 - 7.2, if necessary.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- pseudothermotoga_medium (CultureMech:001625)
- thermotoga_ii_medium (CultureMech:005631)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.380298Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 498 |
| 2026-03-13T03:09:38.757338Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:05.858079Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001625 |
| 2026-03-17T06:30:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:18.007807+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PSEUDOTHERMOTOGA MEDIUM" to "pseudothermotoga_medium" for consistent matching |
| 2026-05-20T06:02:03.146828+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2} |
| 2026-06-05T07:41:42.808946+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.502597+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.726102+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: pseudothermotoga_medium, thermotoga_ii_medium |
| 2026-08-06T03:05:53.726148+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint ec6abb115da3ccc7d4966bcecd7d8429c2265136294a916fa9aba227f2738460 |