Overview
- External ontology
- mediadive.medium:845 (source link unavailable) — SPIRILLUM WINOGRADSKYI MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'spirillum_d427_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:845', 'original_category': 'bacterial'}
- pH range
- {'min': 7.2, 'max': 7.4}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium845.pdf
Composition
flowchart LR
CultureMech_002001["spirillum_winogradskyi_medium"]
ing0_Peptone["Peptone"]
CultureMech_002001 --> ing0_Peptone
ing1_Disodium_succinate["Disodium succinate"]
CultureMech_002001 --> ing1_Disodium_succinate
CHEBI_63675["CHEBI:63675"]
ing1_Disodium_succinate -.-> CHEBI_63675
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_002001 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002001 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002001 --> ing4_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
ing5_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_002001 --> ing5_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing5_MnSO4_x_H2O -.-> CHEBI_86364
ing6_Agar["Agar"]
CultureMech_002001 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Catalase["Catalase"]
CultureMech_002001 --> ing7_Catalase
ing8_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_002001 --> ing8_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing8_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002001 --> ing9_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
ing10_ZnCl2["ZnCl2"]
CultureMech_002001 --> ing10_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing10_ZnCl2 -.-> CHEBI_49976
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002001 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_H3BO3["H3BO3"]
CultureMech_002001 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002001 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002001 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002001 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002001 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_Vitamin_B12["Vitamin B12"]
CultureMech_002001 --> ing17_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing17_Vitamin_B12 -.-> CHEBI_176843
ing18_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002001 --> ing18_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing18_p_Aminobenzoic_acid -.-> CHEBI_30753
ing19_D_____biotin["D-(+)-biotin"]
CultureMech_002001 --> ing19_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing19_D_____biotin -.-> CHEBI_15956
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_002001 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002001 --> ing21_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing21_Calcium_pantothenate -.-> CHEBI_31345
ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002001 --> ing22_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing23_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_002001 --> ing23_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing23_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 4.98504 G_PER_L | ||
| 2 |
Disodium succinate
C4H4O4.2Na 162.052 g/mol
|
0.997009 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.997009 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.997009 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.00199402 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 6 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.00199402 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 7 | Agar | 0.997009 G_PER_L |
CHEBI:2509
agar
|
|
| 8 | Catalase | 1 G_PER_L | ||
| 9 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
5.2 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 10 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 11 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 19 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 20 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 22 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 23 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.3 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 24 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.2 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Preparation
- Dissolve ingredients except trace elements, vitamins and catalase, adjust pH to 7.2 - 7.4, bring medium to the boil and cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. To complete the medium add trace elements, vitamins and catalase from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Vitamins and catalase should be sterilized by filtration. After inoculation, inject sterile air into the culture vessel up to about 20% of the total gas volume (i.e., 4 parts N2 and 1 part air).
- Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- spirillum_d427_medium (CultureMech:006650)
- spirillum_winogradskyi_medium (CultureMech:002001)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:12.478664Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 845 |
| 2026-03-13T06:19:09.282336Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002001 |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.821058+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPIRILLUM WINOGRADSKYI MEDIUM" to "spirillum_winogradskyi_medium" for consistent matching |
| 2026-05-20T06:02:05.522849+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4} |
| 2026-06-05T07:41:46.220427+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.594351+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.028732+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: spirillum_d427_medium, spirillum_winogradskyi_medium |
| 2026-08-06T03:05:56.028767+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 1ca908b55826ebddfa5991aa727727aba8b44bad3d09f63affcdc0d0811ce44c |