modified_haloalkaliphile_medium

CultureMech:002384 bacterial COMPLEX LIQUID
Original name: MODIFIED HALOALKALIPHILE MEDIUM

Overview

External ontology
mediadive.medium:J1215 (source link unavailable) — MODIFIED HALOALKALIPHILE MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'haloalkaliphile_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J306', 'original_category': 'bacterial'}
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1215

Composition

flowchart LR
    CultureMech_002384["modified_haloalkaliphile_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002384 --> ing0_Yeast_extract
    ing1_Casamino_acids["Casamino acids"]
    CultureMech_002384 --> ing1_Casamino_acids
    ing2_Na_glutamate["Na glutamate"]
    CultureMech_002384 --> ing2_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing2_Na_glutamate -.-> CHEBI_64220
    ing3_Trisodium_citrate["Trisodium citrate"]
    CultureMech_002384 --> ing3_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate -.-> CHEBI_53258
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002384 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_002384 --> ing5_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing5_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing6_KCl["KCl"]
    CultureMech_002384 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_NaCl["NaCl"]
    CultureMech_002384 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_Na2CO3["Na2CO3"]
    CultureMech_002384 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Yeast extract 2 G_PER_L
2 Casamino acids 2 G_PER_L
3 Na glutamate
C5H8NO4.Na 169.112 g/mol
1 G_PER_L CHEBI:64220
monosodium glutamate
4 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
0.1 G_PER_L CHEBI:32583
calcium sulfate dihydrate
7 KCl
Cl.K 74.551 g/mol
1 G_PER_L CHEBI:32588
potassium chloride
8 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
9 Na2CO3
CO3.2Na 105.988 g/mol
8 G_PER_L CHEBI:29377
sodium carbonate

Preparation

  1. Add components to distilled water and bring volume to 500 ml.
  2. Add components to distilled water and bring volume to 500 ml.
  3. Autoclave solutions A and B separately and combine them after cooling. For preparation of solid medium, add 18.0 g agar to Solution A (500 ml), combine them after cooling to 60-70C, mix well and quickly distribute into sterile petri dishes or test tubes.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:19.052897Z mediadive-import Imported from MediaDive Source: JCM, ID: J1215
2026-01-28T19:48:14.765481Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:08:52.954982Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:12.903816Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002384
2026-03-17T06:29:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.951739+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED HALOALKALIPHILE MEDIUM" to "modified_haloalkaliphile_medium" for consistent matching
2026-06-05T07:41:36.644825+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:48.784417+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:08.649740+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:37.077683+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: haloalkaliphile_medium, modified_haloalkaliphile_medium
2026-08-06T03:05:37.077730+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 2ae30464c0ad355bcdad0b95404529cd5e2bc5dc1a34fa21631c96647cc85869