Overview
- External ontology
- mediadive.medium:J1270 (source link unavailable) — CHOCOLATE AGAR WITH 5% SHEEP BLOOD
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'brucella_agar', 'source': 'MediaDive', 'source_id': 'mediadive.medium:584', 'original_category': 'bacterial'}
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1270
Composition
flowchart LR
CultureMech_002436["chocolate_agar_with_5_sheep_blood"]
ing0_Brucella_agar["Brucella agar"]
CultureMech_002436 --> ing0_Brucella_agar
ing1_Sheep_blood["Sheep blood"]
CultureMech_002436 --> ing1_Sheep_blood
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Brucella agar | 1000 G_PER_L |
MICRO:0000595 (source link unavailable)
Brucella agar
|
|
| 2 | Sheep blood | 100 G_PER_L |
Preparation
- Mix ingredients except horse blood, autoclave and cool to about 70C. Aseptically add 5% (final) sterile defibrinated horse blood, keep at 70C for 15 minutes and cool to about 50C. Mix and quickly dispense into sterile petri dishes.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- brucella_agar (CultureMech:001710)
- chocolate_agar_with_5_sheep_blood (CultureMech:002436)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.243540Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1270 |
| 2026-03-13T06:19:13.297963Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002436 |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.399207+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CHOCOLATE AGAR WITH 5% SHEEP BLOOD" to "chocolate_agar_with_5_sheep_blood" for consistent matching |
| 2026-06-05T07:40:53.371148+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:25.989067+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: brucella_agar, chocolate_agar_with_5_sheep_blood |
| 2026-08-06T03:05:25.989111+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 8ec12b7e3497d66c9da6c9ad8cbd5ce155d0eef1446fc2599695e257b84f9d9c |