Overview
- External ontology
- mediadive.medium:J386 (source link unavailable) — MIDDLEBROOK 7H10 AGAR WITH OADC ENRICHMENT
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'hemin_medium_for_mycobacterium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J714', 'original_category': 'bacterial'}
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=386
Composition
flowchart LR
CultureMech_002743["middlebrook_7h10_agar_with_oadc_enrichment"]
ing0_Middlebrook_7H10_agar["Middlebrook 7H10 agar"]
CultureMech_002743 --> ing0_Middlebrook_7H10_agar
CHEBI_2509["CHEBI:2509"]
ing0_Middlebrook_7H10_agar -.-> CHEBI_2509
ing1_Glycerol["Glycerol"]
CultureMech_002743 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Middlebrook 7H10 agar | 21.1111 G_PER_L |
CHEBI:2509
agar
|
Copied from referenced JCM Medium 386 |
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
5 G_PER_L |
CHEBI:17754
glycerol
|
Copied from referenced JCM Medium 386 |
Preparation
- Sterilize at 121C for 10 min and cool to 50 - 55C. Aseptically add 100 ml of Middlebrook OADC Enrichment (BD-BBL) and mix thoroughly.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- JCM_J714_HEMIN_MEDIUM_FOR_MYCOBACTERIUM (CultureMech:003061)
- middlebrook_7h10_agar_with_oadc_enrichment (CultureMech:002743)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.472084Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J386 |
| 2026-03-13T06:19:15.424569Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002743 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.820479+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MIDDLEBROOK 7H10 AGAR WITH OADC ENRICHMENT" to "middlebrook_7h10_agar_with_oadc_enrichment" for consistent matching |
| 2026-06-05T07:41:33.901166+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:37.128067+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: JCM_J714_HEMIN_MEDIUM_FOR_MYCOBACTERIUM, middlebrook_7h10_agar_with_oadc_enrichment |
| 2026-08-06T03:04:37.128104+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint adf81a1cf56625a81ca91eb04d6777b6f59df7995129596355599573bdcab692 |