Overview
- External ontology
- mediadive.medium:J714 (source link unavailable) — HEMIN MEDIUM FOR MYCOBACTERIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM Medium 714 | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=714 JCM Medium 714 supplements JCM Medium 386 with 60 micromol/L hemin solubilized in 1 N NaOH.
Composition
flowchart LR
CultureMech_003061["hemin_medium_for_mycobacterium"]
ing0_Middlebrook_7H10_agar__BD_Difco_["Middlebrook 7H10 agar (BD-Difco)"]
CultureMech_003061 --> ing0_Middlebrook_7H10_agar__BD_Difco_
ing1_Glycerol["Glycerol"]
CultureMech_003061 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
ing2_Distilled_water["Distilled water"]
CultureMech_003061 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_Middlebrook_OADC_Enrichment__BD_BBL_["Middlebrook OADC Enrichment (BD-BBL)"]
CultureMech_003061 --> ing3_Middlebrook_OADC_Enrichment__BD_BBL_
ing4_Hemin["Hemin"]
CultureMech_003061 --> ing4_Hemin
CHEBI_50385["CHEBI:50385"]
ing4_Hemin -.-> CHEBI_50385
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Middlebrook 7H10 agar (BD-Difco) | 19 G_PER_L | JCM Medium 386 lists this commercial BD-Difco medium base as supplied. | |
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
5 ML_PER_L |
CHEBI:17754
glycerol
|
JCM Medium 386 lists glycerol as 5 ml/L. |
| 3 |
Distilled water
H2O 18.015 g/mol
|
895 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 386 uses 895 ml/L distilled water before OADC addition. |
| 4 | Middlebrook OADC Enrichment (BD-BBL) | 100 ML_PER_L | JCM Medium 386 aseptically adds this commercial BD-BBL enrichment. | |
| 5 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
60 MICROMOLAR |
CHEBI:50385
hemin
|
JCM Medium 714 adds 60 micromol/L hemin solubilized with 1 N NaOH. |
Preparation
- Sterilize the Middlebrook 7H10 agar, glycerol, and distilled water at 121 C for 10 min.
- Cool to 50-55 C.
- Aseptically add 100 ml/L Middlebrook OADC Enrichment (BD-BBL), add hemin to 60 micromol/L after solubilizing it with 1 N NaOH, and mix thoroughly.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.377838Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J714 |
| 2026-02-03T11:26:55.834670 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:17.505081Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003061 |
| 2026-03-17T19:45:31.222452+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HEMIN MEDIUM FOR MYCOBACTERIUM" to "hemin_medium_for_mycobacterium" for consistent matching |
| 2026-04-04T21:48:41.473924 | resolve_option_c_references | RESOLVED_REFERENCE | Resolved reference by copying from middlebrook_7h10_agar_with_oadc_enrichment (CultureMech:002743) |
| 2026-04-04T22:05:44.144884 | resolve_option_c_references | RESOLVED_REFERENCE | Resolved reference by copying from middlebrook_7h10_agar_with_oadc_enrichment (CultureMech:002743) |
| 2026-05-16T08:57:04.817533+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:40:17.624995+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-10T00:00:00-07:00 | repair_jcm_middlebrook_hemin_score20.py | RESOLVED_JCM_MIDDLEBROOK_HEMIN_SCORE20 | Rebuilt the recipe from live JCM Medium 386 and 714 pages. |