Overview
- External ontology
- mediadive.medium:J565 (source link unavailable) — DESULFOVIBRIO HEB233 MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'desulfotomaculum_lam5_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J545', 'original_category': 'bacterial'}
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=565
Composition
flowchart LR
CultureMech_002914["desulfovibrio_heb233_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002914 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_002914 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_002914 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_002914 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Na2SO4["Na2SO4"]
CultureMech_002914 --> ing4_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing4_Na2SO4 -.-> CHEBI_32149
ing5_KCl["KCl"]
CultureMech_002914 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_CaCl2["CaCl2"]
CultureMech_002914 --> ing6_CaCl2
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2 -.-> CHEBI_3312
ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002914 --> ing7_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
ing8_Yeast_extract["Yeast extract"]
CultureMech_002914 --> ing8_Yeast_extract
ing9_Sodium_lactate["Sodium lactate"]
CultureMech_002914 --> ing9_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing9_Sodium_lactate -.-> CHEBI_75228
ing10_NaHCO3["NaHCO3"]
CultureMech_002914 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002914 --> ing11_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002914 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_Resazurin["Resazurin"]
CultureMech_002914 --> ing13_Resazurin
CHEBI_8806["CHEBI:8806"]
ing13_Resazurin -.-> CHEBI_8806
ing14_HCl["HCl"]
CultureMech_002914 --> ing14_HCl
CHEBI_17883["CHEBI:17883"]
ing14_HCl -.-> CHEBI_17883
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002914 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_H3BO3["H3BO3"]
CultureMech_002914 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002914 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002914 --> ing18_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002914 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002914 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002914 --> ing21_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002914 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.29703 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.29703 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.990099 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.990099 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2.9703 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.0990099 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.0990099 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 8 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.49505 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 9 | Yeast extract | 0.0990099 G_PER_L | ||
| 10 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
0.990099 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.9802 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.49505 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.39604 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000990099 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 15 |
HCl
HCl 36.461 g/mol
|
12.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 19 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix ingredients and adjust pH to 7.2. Distribute 0.9 volume of the medium into tubes (e.g., 4.5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave at 110C for 45 min.
- To complete the medium, add the following stock solutions [Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere] to the medium (per 4.5 ml):
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- desulfotomaculum_lam5_medium (CultureMech:002893)
- desulfovibrio_heb233_medium (CultureMech:002914)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.948839Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J565 |
| 2026-03-13T06:19:16.528725Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002914 |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.092067+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOVIBRIO HEB233 MEDIUM" to "desulfovibrio_heb233_medium" for consistent matching |
| 2026-06-05T07:40:57.816972+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.041082+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:31.989417+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: desulfotomaculum_lam5_medium, desulfovibrio_heb233_medium |
| 2026-08-06T03:05:31.989467+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint f557e3cde1d8ee695aa89ee267f8ccd2902e6d8c9f2eba27ea62a6dad18a65db |