phosphoac3_medium

Overview

External ontology

mediadive.medium:J875 (source link unavailable) — PHOSPHOAC3 MEDIUM

Applications

• Microbial cultivation

Synonyms

• {'name': 'ird_mesotoga_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J926', 'original_category': 'bacterial'}

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=875

Composition

flowchart LR
    CultureMech_003222["phosphoac3_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003222 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_003222 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_003222 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_003222 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_003222 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003222 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003222 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_Sodium_acetate["Sodium acetate"]
    CultureMech_003222 --> ing7_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Sodium_acetate -.-> CHEBI_32954
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_003222 --> ing8_Yeast_extract
    ing9_Sulfur["Sulfur"]
    CultureMech_003222 --> ing9_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing9_Sulfur -.-> CHEBI_26833
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003222 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Resazurin["Resazurin"]
    CultureMech_003222 --> ing11_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing11_Resazurin -.-> CHEBI_8806
    ing12_NaHCO3["NaHCO3"]
    CultureMech_003222 --> ing12_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing12_NaHCO3 -.-> CHEBI_32139
    ing13_L_Sodium_lactate["L-Sodium lactate"]
    CultureMech_003222 --> ing13_L_Sodium_lactate
    CHEBI_232798["CHEBI:232798"]
    ing13_L_Sodium_lactate -.-> CHEBI_232798
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003222 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209
    ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003222 --> ing15_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing16_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003222 --> ing16_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing16_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing17_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003222 --> ing17_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing17_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003222 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003222 --> ing19_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003222 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003222 --> ing21_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing22_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_003222 --> ing22_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing22_AlK_SO4_2 -.-> CHEBI_86463
    ing23_H3BO3["H3BO3"]
    CultureMech_003222 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003222 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (25)

Preparation

1. Mix components except sulfur, and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium into culture vessels containing appropriate amount of sulfur while gassing with a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Add per liter the following solutions (autoclaved and stored under N2, or *filter-sterilized and stored under N2-CO2, 4:1, v/v):
2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

• ird_mesotoga_medium (CultureMech:003273)
• phosphoac3_medium (CultureMech:003222)