Overview
- External ontology
- mediadive.medium:J926 (source link unavailable) — IRD MESOTOGA MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=926
Composition
flowchart LR
CultureMech_003273["ird_mesotoga_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_003273 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_003273 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_003273 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_003273 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KCl["KCl"]
CultureMech_003273 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003273 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003273 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_Sodium_acetate["Sodium acetate"]
CultureMech_003273 --> ing7_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing7_Sodium_acetate -.-> CHEBI_32954
ing8_Yeast_extract["Yeast extract"]
CultureMech_003273 --> ing8_Yeast_extract
ing9_Sulfur["Sulfur"]
CultureMech_003273 --> ing9_Sulfur
CHEBI_26833["CHEBI:26833"]
ing9_Sulfur -.-> CHEBI_26833
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003273 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_Resazurin["Resazurin"]
CultureMech_003273 --> ing11_Resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Resazurin -.-> CHEBI_8806
ing12_NaHCO3["NaHCO3"]
CultureMech_003273 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
ing13_L_Sodium_lactate["L-Sodium lactate"]
CultureMech_003273 --> ing13_L_Sodium_lactate
CHEBI_232798["CHEBI:232798"]
ing13_L_Sodium_lactate -.-> CHEBI_232798
ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003273 --> ing14_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing14_Na2S_x_9_H2O -.-> CHEBI_76209
ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003273 --> ing15_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
ing16_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003273 --> ing16_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing16_MgSO4_x_7_H2O -.-> CHEBI_31795
ing17_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003273 --> ing17_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing17_MnSO4_x_n_H2O -.-> CHEBI_86360
ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003273 --> ing18_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_003273 --> ing19_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003273 --> ing20_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003273 --> ing21_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
ing22_AlK_SO4_2["AlK(SO4)2"]
CultureMech_003273 --> ing22_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing22_AlK_SO4_2 -.-> CHEBI_86463
ing23_H3BO3["H3BO3"]
CultureMech_003273 --> ing23_H3BO3
CHEBI_33118["CHEBI:33118"]
ing23_H3BO3 -.-> CHEBI_33118
ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003273 --> ing24_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28222 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Copied from referenced JCM Medium 875 |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.28222 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Copied from referenced JCM Medium 875 |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.940734 G_PER_L |
CHEBI:31206
ammonium chloride
|
Copied from referenced JCM Medium 875 |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
2.88147 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 1.88147, 1.0] | Copied from JCM Medium 875 |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.0940734 G_PER_L |
CHEBI:32588
potassium chloride
|
Copied from referenced JCM Medium 875 |
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1940734 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.0940734, 0.1] | Copied from JCM Medium 875 |
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.470367 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Copied from referenced JCM Medium 875 |
| 8 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.188147 G_PER_L |
CHEBI:32954
sodium acetate
|
Copied from referenced JCM Medium 875 |
| 9 | Yeast extract | 1.88147 G_PER_L | Copied from referenced JCM Medium 875 | |
| 10 |
Sulfur
S 32.067 g/mol
|
9.40734 G_PER_L |
CHEBI:26833
sulfur atom
|
Copied from referenced JCM Medium 875 |
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.470367 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Copied from referenced JCM Medium 875 |
| 12 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000940734 G_PER_L |
CHEBI:8806
Resazurin
|
Copied from referenced JCM Medium 875 |
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
25 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Copied from referenced JCM Medium 875 |
| 14 |
L-Sodium lactate
C3H5O3.Na 112.06 g/mol
|
20 G_PER_L |
CHEBI:232798
sodium L-lactate
|
Copied from referenced JCM Medium 875 |
| 15 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
8 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Copied from referenced JCM Medium 875 |
| 16 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Copied from referenced JCM Medium 875 |
| 17 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Copied from referenced JCM Medium 875 |
| 18 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Copied from referenced JCM Medium 875 |
| 19 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Copied from referenced JCM Medium 875 |
| 20 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Copied from referenced JCM Medium 875 |
| 21 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Copied from referenced JCM Medium 875 |
| 22 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Copied from referenced JCM Medium 875 |
| 23 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
Copied from referenced JCM Medium 875 |
| 24 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Copied from referenced JCM Medium 875 |
| 25 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Copied from referenced JCM Medium 875 |
Preparation
- Use Medium No. 875, replacing the L-sodium lactate solution with 20.0 ml/L 1.0 M fructose solution (autoclaved and stored under N2).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 2 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:18.027624Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J926 |
| 2026-02-03T11:26:56.639236 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:19.107588Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003273 |
| 2026-03-17T19:45:33.020265+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "IRD MESOTOGA MEDIUM" to "ird_mesotoga_medium" for consistent matching |
| 2026-04-04T18:49:20.108485 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from phosphoac3_medium (CultureMech:003222) |
| 2026-05-16T08:57:35.996760+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:41:09.588948+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.119846+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |