microaerophilic_thermus_medium

CultureMech:003243 bacterial COMPLEX LIQUID
Original name: MICROAEROPHILIC THERMUS MEDIUM

Overview

External ontology
mediadive.medium:J894 (source link unavailable) — MICROAEROPHILIC THERMUS MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'castenholz_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J276', 'original_category': 'bacterial'}
pH
8.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=894

Composition

flowchart LR
    CultureMech_003243["microaerophilic_thermus_medium"]
    ing0_Tryptone["Tryptone"]
    CultureMech_003243 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003243 --> ing1_Yeast_extract
    ing2_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003243 --> ing2_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing2_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing3_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_003243 --> ing3_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing3_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003243 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_NaCl["NaCl"]
    CultureMech_003243 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_KNO3["KNO3"]
    CultureMech_003243 --> ing6_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing6_KNO3 -.-> CHEBI_63043
    ing7_NaNO3["NaNO3"]
    CultureMech_003243 --> ing7_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing7_NaNO3 -.-> CHEBI_63005
    ing8_Na2HPO4["Na2HPO4"]
    CultureMech_003243 --> ing8_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing8_Na2HPO4 -.-> CHEBI_34683
    ing9_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_003243 --> ing9_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing9_FeCl3_x_6_H2O -.-> CHEBI_86254

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Tryptone 1 G_PER_L
2 Yeast extract 1 G_PER_L
3 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
0.980392 G_PER_L CHEBI:44557
nitrilotriacetic acid
4 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
0.588235 G_PER_L CHEBI:32583
calcium sulfate dihydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.980392 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 NaCl
Cl.Na 58.443 g/mol
0.0784314 G_PER_L CHEBI:26710
sodium chloride
7 KNO3
K.NO3 101.102 g/mol
1.0098 G_PER_L CHEBI:63043
potassium nitrate
8 NaNO3
NO3.Na 84.994 g/mol
6.7549 G_PER_L CHEBI:63005
sodium nitrate
9 Na2HPO4
HO4P.2Na 141.958 g/mol
1.08824 G_PER_L CHEBI:34683
disodium hydrogenphosphate
10 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
10 G_PER_L CHEBI:86254
iron trichloride hexahydrate

Preparation

  1. Use Medium No. 276 and adjust pH to 8.0. Cultivate under a N2-O2 (99:1, v/v) gas atmosphere.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:17.927530Z mediadive-import Imported from MediaDive Source: JCM, ID: J894
2026-02-03T11:26:56.519253 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:18.869066Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003243
2026-03-17T19:45:32.753027+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MICROAEROPHILIC THERMUS MEDIUM" to "microaerophilic_thermus_medium" for consistent matching
2026-04-04T18:49:19.340542 copy_referenced_compositions RESOLVED_REFERENCE Resolved reference by copying from castenholz_medium (CultureMech:002633)
2026-05-16T08:58:07.822412+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(2 keys) -> list(1 entries)
2026-06-05T07:41:33.787757+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.591873+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.799426+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:35.954061+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: JCM_J276_CASTENHOLZ_MEDIUM, microaerophilic_thermus_medium
2026-08-06T03:04:35.954100+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 12c902711e41a7fd65fa190169a24a56515b1b9c132d5b0b0bb6e3b3fc6aa032