microaerophilic_thermus_medium (CultureMech:003243)

Overview

External ontology

mediadive.medium:J894 (source link unavailable) — MICROAEROPHILIC THERMUS MEDIUM

Applications

Microbial cultivation

pH

8.0

Notes

JCM Medium 894 / Microaerophilic Thermus Medium uses JCM Medium 276, adjusts the final medium to pH 8.0, and cultivates under a 99:1 N2/O2 gas atmosphere. MediaDive J894 and TOGO M935 report the same JCM recipe.

Composition

flowchart LR
    CultureMech_003243["microaerophilic_thermus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_003243 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_003243 --> ing1_Yeast_extract__BD_Difco_
    ing2_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_003243 --> ing2_Tryptone__BD_Difco_
    ing3_Nitrogen_gas["Nitrogen gas"]
    CultureMech_003243 --> ing3_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing3_Nitrogen_gas -.-> CHEBI_17997
    ing4_Oxygen_gas["Oxygen gas"]
    CultureMech_003243 --> ing4_Oxygen_gas
    CHEBI_15379["CHEBI:15379"]
    ing4_Oxygen_gas -.-> CHEBI_15379

Ingredients (5)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	900.0 ML_PER_L	CHEBI:15377 water	MediaDive J894 / JCM Medium 894 lists 900.0 ml/L Distilled water.
2	Yeast extract (BD-Difco)	1.0 G_PER_L	FOODON:03315426 yeast extract	MediaDive J894 / JCM Medium 894 lists 1.0 g/L Yeast extract (BD-Difco).
3	Tryptone (BD-Difco)	1.0 G_PER_L	MICRO:0000182 (source link unavailable) tryptone	MediaDive J894 / JCM Medium 894 lists 1.0 g/L Tryptone (BD-Difco).
4	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	MediaDive J894 / JCM Medium 894 uses Nitrogen gas in a 99:1 gas atmosphere.
5	Oxygen gas O2 31.998 g/mol	variable VARIABLE	CHEBI:15379 dioxygen	MediaDive J894 / JCM Medium 894 uses Oxygen gas in a 99:1 gas atmosphere.

Preparation

Use the JCM Medium 276 Castenholz formulation with 900.0 ml/L distilled water, 1.0 g/L BD-Difco yeast extract, 1.0 g/L BD-Difco tryptone, and 100.0 ml/L Castenholz basal salt solution.
Adjust pH to 8.0 with NaOH.

Solutions

Castenholz basal salt solution

Dose: 100.0 ML_PER_L

CultureMech:013022

Stock composition
Ingredient	Concentration	Notes
Distilled water	1000.0 ML_PER_L	JCM Medium 273 lists 1000.0 ml/L Distilled water.
MgSO4 x 7 H2O	1.0 G_PER_L	JCM Medium 273 lists 1.0 g/L MgSO4 x 7 H2O.
NaCl	0.08 G_PER_L	JCM Medium 273 lists 0.08 g/L NaCl.
NaNO3	6.89 G_PER_L	JCM Medium 273 lists 6.89 g/L NaNO3.
Nitrilotriacetic acid	1.0 G_PER_L	JCM Medium 273 lists 1.0 g/L Nitrilotriacetic acid.
KNO3	1.03 G_PER_L	JCM Medium 273 lists 1.03 g/L KNO3.
Na2HPO4	1.11 G_PER_L	JCM Medium 273 lists 1.11 g/L Na2HPO4.
CaSO4 x 2 H2O	0.6 G_PER_L	JCM Medium 273 lists 0.6 g/L CaSO4 x 2 H2O.

MediaDive J894 / JCM Medium 894 adds 100.0 ml/L Castenholz basal salt solution defined by JCM Medium 273.

FeCl3 x 6 H2O solution (0.03%)

Dose: 10.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
FeCl3 x 6 H2O	0.03 PERCENT_W_V	JCM Medium 273 lists 0.03 % w/v FeCl3 x 6 H2O.

JCM Medium 273 adds 10.0 ml/L FeCl3 x 6 H2O solution at 0.03% w/v.

Nitsch's trace elements

Dose: 10.0 ML_PER_L

CultureMech:013023

Stock composition
Ingredient	Concentration	Notes
Distilled water	1000.0 ML_PER_L	JCM Medium 273 lists 1000.0 ml/L Distilled water.
Na2MoO4 x 2 H2O	0.025 G_PER_L	JCM Medium 273 lists 0.025 g/L Na2MoO4 x 2 H2O.
H3BO3	0.5 G_PER_L	JCM Medium 273 lists 0.5 g/L H3BO3.
CoCl2 x 6 H2O	0.046 G_PER_L	JCM Medium 273 lists 0.046 g/L CoCl2 x 6 H2O.
ZnSO4 x 7 H2O	0.5 G_PER_L	JCM Medium 273 lists 0.5 g/L ZnSO4 x 7 H2O.
CuSO4 x 5 H2O	0.016 G_PER_L	JCM Medium 273 lists 0.016 g/L CuSO4 x 5 H2O.
MnSO4 x n H2O	2.2 G_PER_L	JCM Medium 273 lists 2.2 g/L MnSO4 x n H2O.
H2SO4	0.5 ML_PER_L	JCM Medium 273 lists 0.5 ml/L H2SO4.

JCM Medium 273 adds 10.0 ml/L Nitsch's trace elements.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:17.927530Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J894
2026-02-03T11:26:56.519253	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:18.869066Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:003243
2026-03-17T19:45:32.753027+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MICROAEROPHILIC THERMUS MEDIUM" to "microaerophilic_thermus_medium" for consistent matching
2026-04-04T18:49:19.340542	copy_referenced_compositions	RESOLVED_REFERENCE	Resolved reference by copying from castenholz_medium (CultureMech:002633)
2026-05-16T08:58:07.822412+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(2 keys) -> list(1 entries)
2026-06-05T07:41:33.787757+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.591873+00:00	primary-term-regrounding-v1.0	Re-grounded wrong primary term id (OAK-verified)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.799426+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-11T00:00:00-07:00	repair_togo_m935_microaerophilic_thermus_score15.py	RESOLVED_MEDIADIVE_J894_MICROAEROPHILIC_THERMUS	Replaced the flattened JCM Medium 276 stock salts with the source 100.0 ml/L Castenholz basal salt solution, corrected JCM Medium 894 to a pH 8.0 variant under 99:1 N2/O2, grounded the gases, and linked TOGO M935 as a source duplicate.