Overview
- External ontology
- mediadive.medium:J276 (source link unavailable) — CASTENHOLZ MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.2
- Notes
- JCM Medium 276 / Castenholz Medium lists 900.0 ml distilled water, 1.0 g/L BD-Difco yeast extract, 1.0 g/L BD-Difco tryptone, and 100.0 ml/L Castenholz basal salt solution from JCM Medium 273, then adjusts the final medium to pH 8.2 with NaOH.
Composition
flowchart LR
CultureMech_002633["castenholz_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_002633 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_002633 --> ing1_Yeast_extract__BD_Difco_
ing2_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_002633 --> ing2_Tryptone__BD_Difco_
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
900.0 ML_PER_L |
CHEBI:15377
water
|
MediaDive J276 / JCM Medium 276 lists 900.0 ml/L Distilled water. |
| 2 | Yeast extract (BD-Difco) | 1.0 G_PER_L |
FOODON:03315426
yeast extract
|
MediaDive J276 / JCM Medium 276 lists 1.0 g/L Yeast extract (BD-Difco). |
| 3 | Tryptone (BD-Difco) | 1.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
MediaDive J276 / JCM Medium 276 lists 1.0 g/L Tryptone (BD-Difco). |
Preparation
- Mix 900.0 ml/L distilled water, 1.0 g/L BD-Difco yeast extract, 1.0 g/L BD-Difco tryptone, and 100.0 ml/L Castenholz basal salt solution.
- Adjust pH to 8.2 with NaOH.
Solutions
Castenholz basal salt solution
Dose: 100.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1000.0 ML_PER_L | JCM Medium 273 lists 1000.0 ml/L Distilled water. |
| MgSO4 x 7 H2O | 1.0 G_PER_L | JCM Medium 273 lists 1.0 g/L MgSO4 x 7 H2O. |
| NaCl | 0.08 G_PER_L | JCM Medium 273 lists 0.08 g/L NaCl. |
| NaNO3 | 6.89 G_PER_L | JCM Medium 273 lists 6.89 g/L NaNO3. |
| Nitrilotriacetic acid | 1.0 G_PER_L | JCM Medium 273 lists 1.0 g/L Nitrilotriacetic acid. |
| KNO3 | 1.03 G_PER_L | JCM Medium 273 lists 1.03 g/L KNO3. |
| Na2HPO4 | 1.11 G_PER_L | JCM Medium 273 lists 1.11 g/L Na2HPO4. |
| CaSO4 x 2 H2O | 0.6 G_PER_L | JCM Medium 273 lists 0.6 g/L CaSO4 x 2 H2O. |
MediaDive J276 / JCM Medium 276 adds 100.0 ml/L Castenholz basal salt solution defined by JCM Medium 273.
FeCl3 x 6 H2O solution (0.03%)
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeCl3 x 6 H2O | 0.03 PERCENT_W_V | JCM Medium 273 lists 0.03 % w/v FeCl3 x 6 H2O. |
JCM Medium 273 adds 10.0 ml/L FeCl3 x 6 H2O solution at 0.03% w/v.
Nitsch's trace elements
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1000.0 ML_PER_L | JCM Medium 273 lists 1000.0 ml/L Distilled water. |
| Na2MoO4 x 2 H2O | 0.025 G_PER_L | JCM Medium 273 lists 0.025 g/L Na2MoO4 x 2 H2O. |
| H3BO3 | 0.5 G_PER_L | JCM Medium 273 lists 0.5 g/L H3BO3. |
| CoCl2 x 6 H2O | 0.046 G_PER_L | JCM Medium 273 lists 0.046 g/L CoCl2 x 6 H2O. |
| ZnSO4 x 7 H2O | 0.5 G_PER_L | JCM Medium 273 lists 0.5 g/L ZnSO4 x 7 H2O. |
| CuSO4 x 5 H2O | 0.016 G_PER_L | JCM Medium 273 lists 0.016 g/L CuSO4 x 5 H2O. |
| MnSO4 x n H2O | 2.2 G_PER_L | JCM Medium 273 lists 2.2 g/L MnSO4 x n H2O. |
| H2SO4 | 0.5 ML_PER_L | JCM Medium 273 lists 0.5 ml/L H2SO4. |
JCM Medium 273 adds 10.0 ml/L Nitsch's trace elements.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.155505Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J276 |
| 2026-03-13T06:19:14.673917Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002633 |
| 2026-03-17T06:30:29.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.966101+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CASTENHOLZ MEDIUM" to "castenholz_medium" for consistent matching |
| 2026-06-05T07:40:16.968664+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.071094+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:42.875520+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m269_castenholz_score15.py | LINKED_TOGO_M269_JCM276_SOURCE_DUPLICATE | Linked TOGO M269 as a source duplicate of JCM Medium 276. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m935_microaerophilic_thermus_score15.py | RESOLVED_MEDIADIVE_J276_CASTENHOLZ_MEDIUM | Restored the JCM Medium 276 direct composition with 900.0 ml/L water, moved the flattened Castenholz stock salts into a 100.0 ml/L nested solution, expanded the Castenholz and Nitsch stock definitions from JCM Medium 273, and linked the JCM Medium 894 pH/atmosphere variant. |