Overview
- External ontology
- komodo.medium:1006 (source link unavailable) — ALLISONELLA medium
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: KOMODO ModelSEED | ID: 1006 | DSMZ Medium: 1006 (mediadive.medium:1006) | Aerobic: Yes Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_003506["allisonella_medium"]
ing0_DL_Histidine["DL-Histidine"]
CultureMech_003506 --> ing0_DL_Histidine
CHEBI_27570["CHEBI:27570"]
ing0_DL_Histidine -.-> CHEBI_27570
ing1_Yeast_extract["Yeast extract"]
CultureMech_003506 --> ing1_Yeast_extract
ing2_Trypticase_peptone["Trypticase peptone"]
CultureMech_003506 --> ing2_Trypticase_peptone
ing3_K2HPO4["K2HPO4"]
CultureMech_003506 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_KH2PO4["KH2PO4"]
CultureMech_003506 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_003506 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_NaCl["NaCl"]
CultureMech_003506 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003506 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003506 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_Sodium_resazurin["Sodium resazurin"]
CultureMech_003506 --> ing9_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Sodium_resazurin -.-> CHEBI_8806
ing10_Na2CO3["Na2CO3"]
CultureMech_003506 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003506 --> ing11_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing12_Acetic_acid["Acetic acid"]
CultureMech_003506 --> ing12_Acetic_acid
CHEBI_15366["CHEBI:15366"]
ing12_Acetic_acid -.-> CHEBI_15366
ing13_Propionic_acid["Propionic acid"]
CultureMech_003506 --> ing13_Propionic_acid
CHEBI_30768["CHEBI:30768"]
ing13_Propionic_acid -.-> CHEBI_30768
ing14_Butyric_acid["Butyric acid"]
CultureMech_003506 --> ing14_Butyric_acid
CHEBI_30772["CHEBI:30772"]
ing14_Butyric_acid -.-> CHEBI_30772
ing15_n_Valeric_acid["n-Valeric acid"]
CultureMech_003506 --> ing15_n_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing15_n_Valeric_acid -.-> CHEBI_17418
ing16_iso_Butyric_acid["iso-Butyric acid"]
CultureMech_003506 --> ing16_iso_Butyric_acid
CHEBI_16135["CHEBI:16135"]
ing16_iso_Butyric_acid -.-> CHEBI_16135
ing17_Tryptone["Tryptone"]
CultureMech_003506 --> ing17_Tryptone
ing18_Yeast_extract["Yeast extract"]
CultureMech_003506 --> ing18_Yeast_extract
ing19_Sodium_chloride["Sodium chloride"]
CultureMech_003506 --> ing19_Sodium_chloride
ing19_Sodium_chloride -.-> CHEBI_26710
ing20_iso_Valeric_acid["iso-Valeric acid"]
CultureMech_003506 --> ing20_iso_Valeric_acid
CHEBI_28484["CHEBI:28484"]
ing20_iso_Valeric_acid -.-> CHEBI_28484
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
DL-Histidine
C6H9N3O2 155.157 g/mol
|
7.76892 G_PER_L |
CHEBI:27570
histidine
|
|
| 2 | Yeast extract | 3.98406 G_PER_L | ||
| 3 | Trypticase peptone | 0.996016 G_PER_L | ||
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.288845 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.288845 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.478088 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
0.478088 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.0996016 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.063745 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000498008 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
3.98406 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.498008 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 13 |
Acetic acid
C2H4O2 60.052 g/mol
|
573.182 G_PER_L |
CHEBI:15366
acetic acid
|
|
| 14 |
Propionic acid
C3H6O2 74.079 g/mol
|
192.145 G_PER_L |
CHEBI:30768
propionic acid
|
|
| 15 |
Butyric acid
C4H8O2 88.106 g/mol
|
129 G_PER_L |
CHEBI:30772
butyric acid
|
|
| 16 |
n-Valeric acid
C5H10O2 102.133 g/mol
|
30.2827 G_PER_L |
CHEBI:17418
valeric acid
|
|
| 17 |
iso-Butyric acid
C4H8O2 88.106 g/mol
|
30.6375 G_PER_L |
CHEBI:16135
isobutyric acid
|
|
| 18 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 19 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 20 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 21 |
iso-Valeric acid
C5H10O2 102.133 g/mol
|
30.0247 G_PER_L |
CHEBI:28484
isovaleric acid
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_1006_ALLISONELLA_medium (CultureMech:003506)
- allisonella_medium (CultureMech:000422)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1006 |
| 2026-02-03T11:19:05.235270 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1006 |
| 2026-03-13T06:19:20.270791Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003506 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:34.379832+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALLISONELLA medium" to "allisonella_medium" for consistent matching |
| 2026-06-05T07:40:18.079524+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.237222+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:37.753400+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_1006_ALLISONELLA_medium, allisonella_medium |
| 2026-08-06T03:04:37.753450+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint e2fabbbb8109828a6ef32604ca9ce5f19604810079a9796f1602a74f080a2559 |