Overview
- External ontology
- komodo.medium:1021 (source link unavailable) — SODALIS GLOSSINIDIUS medium
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_1021_modified_for_dsm_16929', 'source': 'KOMODO', 'source_id': 'komodo.medium:1021_16929', 'original_category': 'bacterial'}
- pH
- 8.0
- Notes
- Source: KOMODO ModelSEED | ID: 1021 | DSMZ Medium: 1021 (mediadive.medium:1021) | Aerobic: No Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_003534["sodalis_glossinidius_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_003534 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_003534 --> ing1_Tryptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_003534 --> ing2_Yeast_extract
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_003534 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_D_Glucose["D-Glucose"]
CultureMech_003534 --> ing4_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing4_D_Glucose -.-> CHEBI_17634
ing5_KCl["KCl"]
CultureMech_003534 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003534 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003534 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_NaCl["NaCl"]
CultureMech_003534 --> ing8_NaCl
ing8_NaCl -.-> CHEBI_26710
ing9_NaHCO3["NaHCO3"]
CultureMech_003534 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Agar["Agar"]
CultureMech_003534 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
ing11_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
CultureMech_003534 --> ing11_NaH2PO4_x_2_H2O
CHEBI_37585["CHEBI:37585"]
ing11_NaH2PO4_x_2_H2O -.-> CHEBI_37585
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 6.88889 G_PER_L | ||
| 2 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 3 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 5 |
D-Glucose
C6H12O6 180.156 g/mol
|
5.55556 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.277778 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.133333 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.277778 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
9.66667 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.166667 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 | Agar | 18.8889 G_PER_L |
CHEBI:2509
agar
|
|
| 12 |
NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
|
2.8 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- KOMODO_1021_SODALIS_GLOSSINIDIUS_medium (CultureMech:003534)
- medium_1021_modified_for_dsm_16929 (CultureMech:003533)
- sodalis_glossinidius_medium (CultureMech:000443)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1021 |
| 2026-02-03T11:19:02.237920 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1021 |
| 2026-03-13T06:19:20.490626Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003534 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:34.633595+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SODALIS GLOSSINIDIUS medium" to "sodalis_glossinidius_medium" for consistent matching |
| 2026-06-05T07:40:18.176442+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:37.916985+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: KOMODO_1021_SODALIS_GLOSSINIDIUS_medium, medium_1021_modified_for_dsm_16929, sodalis_glossinidius_medium |
| 2026-08-06T03:04:37.917042+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 3f16314131bb23eb286ebc16ba69ae7ca5702588ddb9d2c8125891b6e99bd24c |