Overview
- External ontology
- komodo.medium:1245 (source link unavailable) — OTEB-MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_1245_modified_for_dsm_18691', 'source': 'KOMODO', 'source_id': 'komodo.medium:1245_18691', 'original_category': 'bacterial'}
- pH range
- {'min': 6.8, 'max': 7.2}
- Notes
- Source: KOMODO ModelSEED | ID: 1245 | DSMZ Medium: 1245 (mediadive.medium:1245) | Aerobic: No Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_004003["oteb_medium"]
ing0_Proteose_peptone_no__2["Proteose peptone no. 2"]
CultureMech_004003 --> ing0_Proteose_peptone_no__2
ing1_Heart_Infusion_Broth["Heart Infusion Broth"]
CultureMech_004003 --> ing1_Heart_Infusion_Broth
ing2_Yeast_extract["Yeast extract"]
CultureMech_004003 --> ing2_Yeast_extract
ing3_Potassium_Phosphate["Potassium Phosphate"]
CultureMech_004003 --> ing3_Potassium_Phosphate
CHEBI_63036["CHEBI:63036"]
ing3_Potassium_Phosphate -.-> CHEBI_63036
ing4_Sodium_Chloride["Sodium Chloride"]
CultureMech_004003 --> ing4_Sodium_Chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_Chloride -.-> CHEBI_26710
ing5_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_004003 --> ing5_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing5_Magnesium_sulfate -.-> CHEBI_32599
ing6_Acetic_acid["Acetic acid"]
CultureMech_004003 --> ing6_Acetic_acid
CHEBI_15366["CHEBI:15366"]
ing6_Acetic_acid -.-> CHEBI_15366
ing7_Propionic_acid["Propionic acid"]
CultureMech_004003 --> ing7_Propionic_acid
CHEBI_30768["CHEBI:30768"]
ing7_Propionic_acid -.-> CHEBI_30768
ing8_n_Butyric_acid["n-Butyric acid"]
CultureMech_004003 --> ing8_n_Butyric_acid
CHEBI_30772["CHEBI:30772"]
ing8_n_Butyric_acid -.-> CHEBI_30772
ing9_n_Valeric_acid["n-Valeric acid"]
CultureMech_004003 --> ing9_n_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing9_n_Valeric_acid -.-> CHEBI_17418
ing10_Isovaleric_acid["Isovaleric acid"]
CultureMech_004003 --> ing10_Isovaleric_acid
CHEBI_28484["CHEBI:28484"]
ing10_Isovaleric_acid -.-> CHEBI_28484
ing11_Isobutyric_acid["Isobutyric acid"]
CultureMech_004003 --> ing11_Isobutyric_acid
CHEBI_16135["CHEBI:16135"]
ing11_Isobutyric_acid -.-> CHEBI_16135
ing12_Tryptone["Tryptone"]
CultureMech_004003 --> ing12_Tryptone
ing13_Yeast_extract["Yeast extract"]
CultureMech_004003 --> ing13_Yeast_extract
ing14_Sodium_chloride["Sodium chloride"]
CultureMech_004003 --> ing14_Sodium_chloride
ing14_Sodium_chloride -.-> CHEBI_26710
ing15_Vitamin_K1["Vitamin K1"]
CultureMech_004003 --> ing15_Vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing15_Vitamin_K1 -.-> CHEBI_18067
ing16_L_Cysteine["L-Cysteine"]
CultureMech_004003 --> ing16_L_Cysteine
CHEBI_17561["CHEBI:17561"]
ing16_L_Cysteine -.-> CHEBI_17561
ing17_Fetal_bovine_serum["Fetal bovine serum"]
CultureMech_004003 --> ing17_Fetal_bovine_serum
ing18_Glucose["Glucose"]
CultureMech_004003 --> ing18_Glucose
CHEBI_17234["CHEBI:17234"]
ing18_Glucose -.-> CHEBI_17234
ing19_Fructose["Fructose"]
CultureMech_004003 --> ing19_Fructose
CHEBI_28757["CHEBI:28757"]
ing19_Fructose -.-> CHEBI_28757
ing20_Sucrose["Sucrose"]
CultureMech_004003 --> ing20_Sucrose
CHEBI_17992["CHEBI:17992"]
ing20_Sucrose -.-> CHEBI_17992
ing21_Maltose["Maltose"]
CultureMech_004003 --> ing21_Maltose
CHEBI_17306["CHEBI:17306"]
ing21_Maltose -.-> CHEBI_17306
ing22_Ribose["Ribose"]
CultureMech_004003 --> ing22_Ribose
CHEBI_33942["CHEBI:33942"]
ing22_Ribose -.-> CHEBI_33942
ing23_Xylose["Xylose"]
CultureMech_004003 --> ing23_Xylose
CHEBI_18222["CHEBI:18222"]
ing23_Xylose -.-> CHEBI_18222
ing24_Pectin["Pectin"]
CultureMech_004003 --> ing24_Pectin
CHEBI_17309["CHEBI:17309"]
ing24_Pectin -.-> CHEBI_17309
ing25_Starch["Starch"]
CultureMech_004003 --> ing25_Starch
CHEBI_28017["CHEBI:28017"]
ing25_Starch -.-> CHEBI_28017
ing26_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_004003 --> ing26_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing26_Sodium_pyruvate -.-> CHEBI_50144
ing27_Thiamine_pyrophosphate["Thiamine pyrophosphate"]
CultureMech_004003 --> ing27_Thiamine_pyrophosphate
CHEBI_18290["CHEBI:18290"]
ing27_Thiamine_pyrophosphate -.-> CHEBI_18290
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Proteose peptone no. 2 | 5 G_PER_L | ||
| 2 | Heart Infusion Broth | 5 G_PER_L | ||
| 3 | Yeast extract | 5 G_PER_L | ||
| 4 |
Potassium Phosphate
H2O4P.K 136.084 g/mol
|
2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
(dibasic) |
| 5 |
Sodium Chloride
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.1 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 7 |
Acetic acid
C2H4O2 60.052 g/mol
|
1.6 G_PER_L |
CHEBI:15366
acetic acid
|
|
| 8 |
Propionic acid
C3H6O2 74.079 g/mol
|
0.6 G_PER_L |
CHEBI:30768
propionic acid
|
|
| 9 |
n-Butyric acid
C4H8O2 88.106 g/mol
|
0.4 G_PER_L |
CHEBI:30772
butyric acid
|
|
| 10 |
n-Valeric acid
C5H10O2 102.133 g/mol
|
0.1 G_PER_L |
CHEBI:17418
valeric acid
|
|
| 11 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
0.1 G_PER_L |
CHEBI:28484
isovaleric acid
|
|
| 12 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
0.1 G_PER_L |
CHEBI:16135
isobutyric acid
|
|
| 13 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 14 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 15 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 16 |
Vitamin K1
C31H46O2 450.707 g/mol
|
0.001 G_PER_L |
CHEBI:18067
phylloquinone
|
|
| 17 |
L-Cysteine
C3H7NO2S 121.161 g/mol
|
0.5 G_PER_L |
CHEBI:17561
L-cysteine
|
|
| 18 | Fetal bovine serum | 100 G_PER_L | (inactivated) | |
| 19 |
Glucose
C6H12O6 180.156 g/mol
|
8 G_PER_L |
CHEBI:17234
glucose
|
|
| 20 | Fructose | 8 G_PER_L |
CHEBI:28757
fructose
|
|
| 21 |
Sucrose
C12H22O11 342.297 g/mol
|
8 G_PER_L |
CHEBI:17992
sucrose
|
|
| 22 |
Maltose
C12H22O11 342.297 g/mol
|
16.0 G_PER_L |
CHEBI:17306
maltose
|
[Merged 2 duplicates: 8.0, 8.0] |
| 23 |
Ribose
C5H10O5 150.13 g/mol
|
8 G_PER_L |
CHEBI:33942
ribose
|
|
| 24 |
Xylose
C5H10O5 150.13 g/mol
|
8 G_PER_L |
CHEBI:18222
xylose
|
|
| 25 | Pectin | 8 G_PER_L |
CHEBI:17309
pectin
|
|
| 26 | Starch | 8 G_PER_L |
CHEBI:28017
starch
|
|
| 27 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
8 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 28 |
Thiamine pyrophosphate
C12H19N4O7P2S.Cl 460.773 g/mol
|
0.00075 G_PER_L |
CHEBI:18290
thiamine(1+) diphosphate chloride
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- KOMODO_1245_OTEB-MEDIUM (CultureMech:004003)
- medium_1245_modified_for_dsm_18691 (CultureMech:004002)
- oteb_medium (CultureMech:000704)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1245 |
| 2026-02-03T11:18:59.204026 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1245 |
| 2026-03-13T03:08:49.825227Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:24.042755Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004003 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:38.698456+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "OTEB-MEDIUM" to "oteb_medium" for consistent matching |
| 2026-05-20T06:00:49.748820+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7.2' -> {'min': 6.8, 'max': 7.2} |
| 2026-06-05T07:40:20.369946+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:42.360519+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: KOMODO_1245_OTEB-MEDIUM, medium_1245_modified_for_dsm_18691, oteb_medium |
| 2026-08-06T03:04:42.360573+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 391f43a9339d9f56eaba3372f64fb2f18bd12277abf3112dd25812588e4611cf |