Overview
- External ontology
- komodo.medium:265 (source link unavailable) — THERMOFILUM PENDENS medium
- Applications
-
- Microbial cultivation
- pH
- 5.2
- Notes
- Source: KOMODO ModelSEED | ID: 265 | DSMZ Medium: 265 (mediadive.medium:265) | Aerobic: Yes
Composition
flowchart LR
CultureMech_004668["thermofilum_pendens_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_004668 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_004668 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004668 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004668 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_004668 --> ing4_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004668 --> ing5_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
ing6_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_004668 --> ing6_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing6_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_004668 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_004668 --> ing8_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing8_CuCl2_x_2_H2O -.-> CHEBI_86318
ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004668 --> ing9_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing10_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
CultureMech_004668 --> ing10_VOSO4_x_2_H2O
CHEBI_87009["CHEBI:87009"]
ing10_VOSO4_x_2_H2O -.-> CHEBI_87009
ing11_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_004668 --> ing11_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing11_CoSO4_x_7_H2O -.-> CHEBI_91244
ing12_Yeast_extract["Yeast extract"]
CultureMech_004668 --> ing12_Yeast_extract
ing13_Sucrose["Sucrose"]
CultureMech_004668 --> ing13_Sucrose
CHEBI_17992["CHEBI:17992"]
ing13_Sucrose -.-> CHEBI_17992
ing14_Sulfur["Sulfur"]
CultureMech_004668 --> ing14_Sulfur
CHEBI_26833["CHEBI:26833"]
ing14_Sulfur -.-> CHEBI_26833
ing15_Polar_lipid_fraction["Polar lipid fraction"]
CultureMech_004668 --> ing15_Polar_lipid_fraction
ing16_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_004668 --> ing16_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing16_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.02 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 6 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0018 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 7 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0045 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.00022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 9 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
5e-05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 10 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3e-05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 11 |
VOSO4 x 2 H2O
2H2O.O4S.OV 199.036 g/mol
|
3e-05 G_PER_L |
CHEBI:87009
vanadyl sulfate dihydrate
|
|
| 12 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
1e-05 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 13 | Yeast extract | 0.02 G_PER_L | (boiled and filter-sterilized, not autoclaved) | |
| 14 |
Sucrose
C12H22O11 342.297 g/mol
|
2 G_PER_L |
CHEBI:17992
sucrose
|
|
| 15 |
Sulfur
S 32.067 g/mol
|
10 G_PER_L |
CHEBI:26833
sulfur atom
|
(sterilized by steaming for 3 hours on 3 consecutive days) |
| 16 | Polar lipid fraction | 10 G_PER_L | (adjust if required) | |
| 17 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_265_THERMOFILUM_PENDENS_medium (CultureMech:004668)
- thermofilum_pendens_medium (CultureMech:001364)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 265 |
| 2026-02-03T11:19:07.862897 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 265 |
| 2026-03-13T06:19:27.968011Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004668 |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:43.280238+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "THERMOFILUM PENDENS medium" to "thermofilum_pendens_medium" for consistent matching |
| 2026-06-05T07:40:21.973109+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.473468+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:22.746603+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_265_THERMOFILUM_PENDENS_medium, thermofilum_pendens_medium |
| 2026-08-06T03:04:22.746643+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint aebd34c16d3c71c7ed08b63947d013e82685d8cf3173099060d1eeab18e6e82a |