Overview
- External ontology
- komodo.medium:436 (source link unavailable) — RUMINOCOCCUS ALBUS medium
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_436_modified_for_dsm_18848', 'source': 'KOMODO', 'source_id': 'komodo.medium:436_18848', 'original_category': 'bacterial'}
- pH
- 7.0
- Notes
- Source: KOMODO ModelSEED | ID: 436 | DSMZ Medium: 436 (mediadive.medium:436) | Aerobic: Yes Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_005284["ruminococcus_albus_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_005284 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_005284 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_005284 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Cellobiose["Cellobiose"]
CultureMech_005284 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Resazurin["Resazurin"]
CultureMech_005284 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Na2CO3["Na2CO3"]
CultureMech_005284 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_005284 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_K2HPO4["K2HPO4"]
CultureMech_005284 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_KH2PO4["KH2PO4"]
CultureMech_005284 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9__NH4_2SO4["(NH4)2SO4"]
CultureMech_005284 --> ing9__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing9__NH4_2SO4 -.-> CHEBI_62946
ing10_NaCl["NaCl"]
CultureMech_005284 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005284 --> ing11_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
ing12_CaCl2_x_7_H2O["CaCl2 x 7 H2O"]
CultureMech_005284 --> ing12_CaCl2_x_7_H2O
CHEBI_3312["CHEBI:3312"]
ing12_CaCl2_x_7_H2O -.-> CHEBI_3312
ing13_Isobutyric_acid["Isobutyric acid"]
CultureMech_005284 --> ing13_Isobutyric_acid
CHEBI_16135["CHEBI:16135"]
ing13_Isobutyric_acid -.-> CHEBI_16135
ing14_Isovaleric_acid["Isovaleric acid"]
CultureMech_005284 --> ing14_Isovaleric_acid
CHEBI_28484["CHEBI:28484"]
ing14_Isovaleric_acid -.-> CHEBI_28484
ing15_Tryptone["Tryptone"]
CultureMech_005284 --> ing15_Tryptone
ing16_Yeast_extract["Yeast extract"]
CultureMech_005284 --> ing16_Yeast_extract
ing17_Sodium_chloride["Sodium chloride"]
CultureMech_005284 --> ing17_Sodium_chloride
ing17_Sodium_chloride -.-> CHEBI_26710
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 5 G_PER_L | ||
| 2 | Yeast extract | 2 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
3 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
2 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
4 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
20 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
12 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 12 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 13 |
CaCl2 x 7 H2O
Ca.2Cl 110.984 g/mol
|
1.6 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 14 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
10 G_PER_L |
CHEBI:16135
isobutyric acid
|
|
| 15 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
10 G_PER_L |
CHEBI:28484
isovaleric acid
|
|
| 16 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 17 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 18 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- KOMODO_436_RUMINOCOCCUS_ALBUS_medium (CultureMech:005284)
- medium_436_modified_for_dsm_18848 (CultureMech:005283)
- ruminococcus_albus_medium (CultureMech:001544)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 436 |
| 2026-02-03T11:18:56.585069 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 436 |
| 2026-03-13T06:19:31.503897Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005284 |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:47.393510+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RUMINOCOCCUS ALBUS medium" to "ruminococcus_albus_medium" for consistent matching |
| 2026-06-05T07:40:23.333256+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.895235+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:49.364480+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: KOMODO_436_RUMINOCOCCUS_ALBUS_medium, medium_436_modified_for_dsm_18848, ruminococcus_albus_medium |
| 2026-08-06T03:04:49.364537+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 388724ba9956388794dc993cd77fe50898f67586530f3b5e1db8c5d8a1e95bee |