Overview
- External ontology
- komodo.medium:460 (source link unavailable) — NITROGEN-FREE medium FOR PSEUDOMONAS STUTZERI
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_460_modified_for_dsm_4166', 'source': 'KOMODO', 'source_id': 'komodo.medium:460_4166', 'original_category': 'bacterial'}
- pH
- 7.0
- Notes
- Source: KOMODO ModelSEED | ID: 460 | DSMZ Medium: 460 (mediadive.medium:460) | Aerobic: No
Composition
flowchart LR
CultureMech_005466["nitrogen_free_medium_for_pseudomonas_stutzeri"]
ing0_K2HPO4["K2HPO4"]
CultureMech_005466 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005466 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_005466 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Yeast_extract["Yeast extract"]
CultureMech_005466 --> ing3_Yeast_extract
ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_005466 --> ing4_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
ing5_DL_Na_malate["DL-Na-malate"]
CultureMech_005466 --> ing5_DL_Na_malate
CHEBI_91260["CHEBI:91260"]
ing5_DL_Na_malate -.-> CHEBI_91260
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 | Yeast extract | 0.2 G_PER_L | ||
| 5 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.015 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 6 | DL-Na-malate | 6.6 G_PER_L |
CHEBI:91260
disodium malate
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- KOMODO_460_NITROGEN-FREE_medium_FOR_PSEUDOMONAS_STUTZERI (CultureMech:005466)
- medium_460_modified_for_dsm_4166 (CultureMech:005465)
- nitrogen_free_medium_for_pseudomonas_stutzeri (CultureMech:001574)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 460 |
| 2026-02-03T11:19:13.778326 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 460 |
| 2026-03-13T06:19:33.146658Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005466 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:49.216620+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NITROGEN-FREE medium FOR PSEUDOMONAS STUTZERI" to "nitrogen_free_medium_for_pseudomonas_stutzeri" for consistent matching |
| 2026-06-05T07:40:23.512442+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.952375+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:51.664151+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: KOMODO_460_NITROGEN-FREE_medium_FOR_PSEUDOMONAS_STUTZERI, medium_460_modified_for_dsm_4166, nitrogen_free_medium_for_pseudomonas_stutzeri |
| 2026-08-06T03:04:51.664188+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint e86db2fa12b41b09cd989a0a72e31861bb3d463e8040b4ca9ffe28c3973b8e49 |