desulfurobacterium_medium

CultureMech:006564 bacterial COMPLEX LIQUID
Original name: DESULFUROBACTERIUM MEDIUM

Overview

External ontology
komodo.medium:829 (source link unavailable) — DESULFUROBACTERIUM MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'desulfurobacterium_atlanticum_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:829a', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_14290', 'source': 'KOMODO', 'source_id': 'komodo.medium:829.1', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_15218_dsm_15521_dsm_15522_dsm_16304', 'source': 'KOMODO', 'source_id': 'komodo.medium:829.2', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_21157', 'source': 'KOMODO', 'source_id': 'komodo.medium:829.3', 'original_category': 'bacterial'}
  • {'name': 'medium_829_modified_for_dsm_16661', 'source': 'KOMODO', 'source_id': 'komodo.medium:829_16661', 'original_category': 'bacterial'}
pH
6.0
Notes
Source: KOMODO ModelSEED | ID: 829 | DSMZ Medium: 829 (mediadive.medium:829) | Aerobic: Yes Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_006564["desulfurobacterium_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_006564 --> ing0_Sea_Salt
    ing1_NH4Cl["NH4Cl"]
    CultureMech_006564 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_KH2PO4["KH2PO4"]
    CultureMech_006564 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MES__2__N_morpholino__ethane_sulfonic_acid_["MES [2-(N-morpholino) ethane sulfonic acid]"]
    CultureMech_006564 --> ing3_MES__2__N_morpholino__ethane_sulfonic_acid_
    CHEBI_39005["CHEBI:39005"]
    ing3_MES__2__N_morpholino__ethane_sulfonic_acid_ -.-> CHEBI_39005
    ing4_Sodium_resazurin["Sodium resazurin"]
    CultureMech_006564 --> ing4_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Sodium_resazurin -.-> CHEBI_8806
    ing5_Sulfur["Sulfur"]
    CultureMech_006564 --> ing5_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing5_Sulfur -.-> CHEBI_26833
    ing6_Na2CO3["Na2CO3"]
    CultureMech_006564 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377
    ing7_Isobutyric_acid["Isobutyric acid"]
    CultureMech_006564 --> ing7_Isobutyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing7_Isobutyric_acid -.-> CHEBI_16135
    ing8_Valeric_acid["Valeric acid"]
    CultureMech_006564 --> ing8_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing8_Valeric_acid -.-> CHEBI_17418
    ing9_Tryptone["Tryptone"]
    CultureMech_006564 --> ing9_Tryptone
    ing10_Yeast_extract["Yeast extract"]
    CultureMech_006564 --> ing10_Yeast_extract
    ing11_Sodium_chloride["Sodium chloride"]
    CultureMech_006564 --> ing11_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing11_Sodium_chloride -.-> CHEBI_26710
    ing12_Caproic_acid["Caproic acid"]
    CultureMech_006564 --> ing12_Caproic_acid
    CHEBI_30776["CHEBI:30776"]
    ing12_Caproic_acid -.-> CHEBI_30776
    ing13_Succinic_acid["Succinic acid"]
    CultureMech_006564 --> ing13_Succinic_acid
    CHEBI_15741["CHEBI:15741"]
    ing13_Succinic_acid -.-> CHEBI_15741
    ing14_HCl["HCl"]
    CultureMech_006564 --> ing14_HCl
    CHEBI_17883["CHEBI:17883"]
    ing14_HCl -.-> CHEBI_17883
    ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_006564 --> ing15_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing16_ZnCl2["ZnCl2"]
    CultureMech_006564 --> ing16_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing16_ZnCl2 -.-> CHEBI_49976
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_006564 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_H3BO3["H3BO3"]
    CultureMech_006564 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_006564 --> ing19_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_006564 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_006564 --> ing21_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_006564 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing23_NaOH["NaOH"]
    CultureMech_006564 --> ing23_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing23_NaOH -.-> CHEBI_32145
    ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_006564 --> ing24_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing25_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_006564 --> ing25_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing25_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing26_Vitamin_B12["Vitamin B12"]
    CultureMech_006564 --> ing26_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing26_Vitamin_B12 -.-> CHEBI_176843
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_006564 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_D_____biotin["D-(+)-biotin"]
    CultureMech_006564 --> ing28_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing28_D_____biotin -.-> CHEBI_15956
    ing29_Nicotinic_acid["Nicotinic acid"]
    CultureMech_006564 --> ing29_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing29_Nicotinic_acid -.-> CHEBI_15940
    CultureMech_006564 --> more["...4 more"]

Ingredients (34)

# Ingredient Concentration Ontology Notes
1 Sea Salt 29.8507 G_PER_L
2 NH4Cl
Cl.H4N 53.492 g/mol
0.995025 G_PER_L CHEBI:31206
ammonium chloride
3 KH2PO4
H2O4P.K 136.084 g/mol
0.348259 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 MES [2-(N-morpholino) ethane sulfonic acid]
C6H13NO4S 195.24 g/mol
1.9403 G_PER_L CHEBI:39005
2-(N-morpholino)ethanesulfonic acid
5 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000497512 G_PER_L CHEBI:8806
Resazurin
6 Sulfur
S 32.067 g/mol
9.95025 G_PER_L CHEBI:26833
sulfur atom
 (powdered)
7 Na2CO3
CO3.2Na 105.988 g/mol
0.497512 G_PER_L CHEBI:29377
sodium carbonate
8 Isobutyric acid
C4H8O2 88.106 g/mol
5 G_PER_L CHEBI:16135
isobutyric acid
9 Valeric acid
C5H10O2 102.133 g/mol
5 G_PER_L CHEBI:17418
valeric acid
10 Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
10.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
11 Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
5.0 G_PER_L FOODON:03315426
yeast extract
 Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
12 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
10.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
13 Caproic acid
C6H12O2 116.16 g/mol
2 G_PER_L CHEBI:30776
hexanoic acid
14 Succinic acid
C4H6O4 118.088 g/mol
6 G_PER_L CHEBI:15741
succinic acid
15 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
16 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
17 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
18 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
19 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
20 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
24 NaOH
HO.Na 39.997 g/mol
1.0 G_PER_L CHEBI:32145
sodium hydroxide
 [Merged 2 duplicates: 0.5, 0.5]
25 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
26 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
27 Vitamin B12 0.1 G_PER_L CHEBI:176843
vitamin B12
28 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
29 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
30 Nicotinic acid
C6H5NO2 123.111 g/mol
0.2 G_PER_L CHEBI:15940
nicotinic acid
31 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate
32 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.3 G_PER_L CHEBI:30961
pyridoxine hydrochloride
33 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.2 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
34 Na2S2O4
2Na.O4S2 174.11 g/mol
50 G_PER_L CHEBI:66870
sodium dithionite

Target organisms

No target organisms are recorded.

Provenance

Merged from 7 source records.

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 829
2026-02-03T11:19:17.927273 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 829
2026-03-13T03:09:39.491419Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:41.959862Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006564
2026-03-17T06:30:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:59.493736+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFUROBACTERIUM MEDIUM" to "desulfurobacterium_medium" for consistent matching
2026-06-05T07:40:26.824999+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:46.021724+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:00.023200+00:00 recipe-merger Merged 7 duplicate recipes into canonical record Sources: KOMODO_829_DESULFUROBACTERIUM_MEDIUM, KOMODO_829a_DESULFUROBACTERIUM_ATLANTICUM_MEDIUM, desulfurobacterium_medium, for_dsm_14290, for_dsm_15218_dsm_15521_dsm_15522_dsm_16304, for_dsm_21157, medium_829_modified_for_dsm_16661
2026-08-06T03:05:00.023317+00:00 merge_recipes.py MERGED_RECIPES merged 7 source recipe(s) on fingerprint 497ff9b2e96b13693502789556e4328fb923be4fb6ad879cbf4115b5b0d98e64
2026-08-15T20:09:01.980352+00:00 dedupe_identical_ingredients.py REMOVED_DUPLICATE_INGREDIENT_ROWS Removed 3 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Sodium chloride, Tryptone, Yeast extract.