Overview
- External ontology
- komodo.medium:951 (source link unavailable) — METHYLOPHAGA SULFIDOVORANS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.2, 'max': 7.8}
- Notes
- pH buffer: HCl | Source: KOMODO ModelSEED | ID: 951 | DSMZ Medium: 951 (mediadive.medium:951) | Aerobic: No
Composition
flowchart LR
CultureMech_006894["methylophaga_sulfidovorans_medium"]
ing0_NaCl["NaCl"]
CultureMech_006894 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_006894 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
CultureMech_006894 --> ing2_CaCl2_x_6_H2O
CHEBI_91243["CHEBI:91243"]
ing2_CaCl2_x_6_H2O -.-> CHEBI_91243
ing3_KCl["KCl"]
CultureMech_006894 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006894 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_KH2PO4["KH2PO4"]
CultureMech_006894 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Na2CO3["Na2CO3"]
CultureMech_006894 --> ing6_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing6_Na2CO3 -.-> CHEBI_29377
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_006894 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
ing8_Methanol["Methanol"]
CultureMech_006894 --> ing8_Methanol
CHEBI_17790["CHEBI:17790"]
ing8_Methanol -.-> CHEBI_17790
ing9_Agar["Agar"]
CultureMech_006894 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_HCl["HCl"]
CultureMech_006894 --> ing10_HCl
CHEBI_17883["CHEBI:17883"]
ing10_HCl -.-> CHEBI_17883
ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_006894 --> ing11_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
ing12_ZnCl2["ZnCl2"]
CultureMech_006894 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_006894 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_006894 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006894 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_006894 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_006894 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006894 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_Biotin["Biotin"]
CultureMech_006894 --> ing19_Biotin
CHEBI_15956["CHEBI:15956"]
ing19_Biotin -.-> CHEBI_15956
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_006894 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Thiamine["Thiamine"]
CultureMech_006894 --> ing21_Thiamine
CHEBI_18385["CHEBI:18385"]
ing21_Thiamine -.-> CHEBI_18385
ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_006894 --> ing22_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
ing23_Pantothenate["Pantothenate"]
CultureMech_006894 --> ing23_Pantothenate
CHEBI_16454["CHEBI:16454"]
ing23_Pantothenate -.-> CHEBI_16454
ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_006894 --> ing24_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing25_Riboflavine["Riboflavine"]
CultureMech_006894 --> ing25_Riboflavine
CHEBI_17015["CHEBI:17015"]
ing25_Riboflavine -.-> CHEBI_17015
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_006894 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
15 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
|
0.33 G_PER_L |
CHEBI:91243
calcium chloride hexahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.02 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Na2CO3
CO3.2Na 105.988 g/mol
|
2 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.001 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 9 |
Methanol
CH4O 32.042 g/mol
|
7.92 G_PER_L |
CHEBI:17790
methanol
|
|
| 10 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 11 |
HCl
HCl 36.461 g/mol
|
5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 12 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.49254 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.0696517 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.199005 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.00597015 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.189055 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.00199005 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.0238806 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0358209 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 22 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.1 G_PER_L |
CHEBI:18385
thiamine(1+)
|
|
| 23 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.1 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 24 |
Pantothenate
C9H16NO5 218.229 g/mol
|
0.05 G_PER_L |
CHEBI:16454
pantothenate
|
|
| 25 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.5 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 26 |
Riboflavine
C17H20N4O6 376.369 g/mol
|
0.01 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 27 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- KOMODO_951_METHYLOPHAGA_SULFIDOVORANS_MEDIUM (CultureMech:006894)
- methylophaga_sulfidovorans_medium (CultureMech:002129)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 951 |
| 2026-02-03T11:18:58.148754 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 951 |
| 2026-03-13T06:19:44.726534Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006894 |
| 2026-03-17T06:29:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:02.699080+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOPHAGA SULFIDOVORANS MEDIUM" to "methylophaga_sulfidovorans_medium" for consistent matching |
| 2026-05-20T06:00:55.086918+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.2-7.8' -> {'min': 7.2, 'max': 7.8} |
| 2026-06-05T07:40:28.125059+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.507152+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:02.740799+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: KOMODO_951_METHYLOPHAGA_SULFIDOVORANS_MEDIUM, methylophaga_sulfidovorans_medium |
| 2026-08-06T03:05:02.740843+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint cab9009d263e1495895bee89431dc5e036d6d97650047dd8b962bc9ecdcbbe65 |