modified_halobacteria_medium_3

CultureMech:007582 archaea COMPLEX LIQUID
Original name: Modified Halobacteria Medium-3

Overview

External ontology
TOGO:M1065 — Modified Halobacteria Medium-3
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'halobacteria_medium', 'source': 'TOGO', 'source_id': 'TOGO:M159', 'original_category': 'archaea'}
  • {'name': 'modified_halobacteria_medium', 'source': 'TOGO', 'source_id': 'TOGO:M576', 'original_category': 'archaea'}
  • {'name': 'halobacterium_tmm_mediium', 'source': 'TOGO', 'source_id': 'TOGO:M817', 'original_category': 'archaea'}
  • {'name': 'halobacteria_medium_with_15_nacl', 'source': 'TOGO', 'source_id': 'TOGO:M959', 'original_category': 'archaea'}
  • {'name': 'lentibacillus_medium', 'source': 'TOGO', 'source_id': 'TOGO:M371', 'original_category': 'bacterial'}
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M1065 Original source: JCM - JCM_M1008 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1008

Composition

flowchart LR
    CultureMech_007582["modified_halobacteria_medium_3"]
    ing0_distilled_water["distilled water"]
    CultureMech_007582 --> ing0_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007582 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007582 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KCl["KCl"]
    CultureMech_007582 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MnCl2_4H2O["MnCl2・4H2O"]
    CultureMech_007582 --> ing4_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing4_MnCl2_4H2O -.-> CHEBI_86368
    ing5_Sodium_glutamate["Sodium glutamate"]
    CultureMech_007582 --> ing5_Sodium_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing5_Sodium_glutamate -.-> CHEBI_64220
    ing6_Trisodium_citrate["Trisodium citrate"]
    CultureMech_007582 --> ing6_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing6_Trisodium_citrate -.-> CHEBI_53258
    ing7_FeCl2_4H2O["FeCl2・4H2O"]
    CultureMech_007582 --> ing7_FeCl2_4H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_4H2O -.-> CHEBI_86249
    ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007582 --> ing8_Yeast_extract__BD_Difco_
    ing9_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
    CultureMech_007582 --> ing9_Casamino_acids__BD_Difco_

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
15 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 NaCl
Cl.Na 58.443 g/mol
270 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
4 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
5 MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
0.36 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
6 Sodium glutamate
C5H8NO4.Na 169.112 g/mol
1 G_PER_L CHEBI:64220
monosodium glutamate
 Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component
7 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
 Role: Buffer; Properties: Defined component, Organic compound, Simple component
8 FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
36 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
9 Yeast extract (BD-Difco) 5 G_PER_L Properties: Undefined component
10 Casamino acids (BD-Difco) 5 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

Merged from 6 source records.

TimestampCuratorActionNotes
2026-01-27T06:35:53.857192Z togo-import Imported from TOGO Medium Source: JCM, ID: M1065
2026-01-28T19:48:39.841252Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:49.842335Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007582
2026-03-17T06:30:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.194343+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Halobacteria Medium-3" to "modified_halobacteria_medium_3" for consistent matching
2026-06-05T07:40:30.134609+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:21.004181+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-08-06T03:04:24.491574+00:00 recipe-merger Merged 6 duplicate recipes into canonical record Sources: TOGO_M1065_Modified_Halobacteria_Medium-3, TOGO_M159_Halobacteria_Medium, TOGO_M576_Modified_Halobacteria_Medium, TOGO_M817_Halobacterium_TMM_Mediium, TOGO_M959_Halobacteria_Medium_With_15_NaCl, TOGO_M371_Lentibacillus_Medium
2026-08-06T03:04:24.491644+00:00 merge_recipes.py MERGED_RECIPES merged 6 source recipe(s) on fingerprint d1f12b660ac881394f627e9e194da6a238d34c9066db0c785564813ffac65593