glycerol_asparagine_agar_isp_medium_no_5

CultureMech:008027 bacterial COMPLEX SOLID_AGAR
Original name: Glycerol-Asparagine Agar (ISP Medium No.5)

Overview

External ontology
TOGO:M1483 — Glycerol-Asparagine Agar (ISP Medium No.5)
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'glycerol_asparagine_agar_isp_5', 'source': 'TOGO', 'source_id': 'TOGO:M52', 'original_category': 'bacterial'}
  • {'name': 'acidic_glycerol_asparagine_agar_isp_medium_no_5', 'source': 'TOGO', 'source_id': 'TOGO:M1642', 'original_category': 'bacterial'}
Notes
Source: https://togomedium.org/medium/M1483 Original source: NBRC - NBRC_M265 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=265

Composition

flowchart LR
    CultureMech_008027["glycerol_asparagine_agar_isp_medium_no_5"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008027 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_K2HPO4["K2HPO4"]
    CultureMech_008027 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_Glycerol["Glycerol"]
    CultureMech_008027 --> ing2_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing2_Glycerol -.-> CHEBI_17754
    ing3_L__Asparagine["L--Asparagine"]
    CultureMech_008027 --> ing3_L__Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing3_L__Asparagine -.-> CHEBI_17196
    ing4_Agar["Agar"]
    CultureMech_008027 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_008027 --> ing5_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_7H2O -.-> CHEBI_75836
    ing6_MnCl2_4H2O["MnCl2・4H2O"]
    CultureMech_008027 --> ing6_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_4H2O -.-> CHEBI_86368
    ing7_ZnSO4_7H2O["ZnSO4・7H2O"]
    CultureMech_008027 --> ing7_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_7H2O -.-> CHEBI_32312

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
101.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 1.0, 100.0]
2 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
3 Glycerol
C3H8O3 92.094 g/mol
10 G_PER_L CHEBI:17754
glycerol
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
4 L--Asparagine
C4H8N2O3 132.119 g/mol
1 G_PER_L CHEBI:17196
L-asparagine
 Role: Nitrogen source; Properties: Simple component, Organic compound, Defined component
5 Agar 15 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
6 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
7 MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
8 ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component

Solutions

Trace salts solution*

Dose: 1 G_PER_L

See mediadive_3683_Trace_salts_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 3 source records.

TimestampCuratorActionNotes
2026-01-27T06:35:57.741229Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1483
2026-01-28T19:48:15.233018Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:25.378607+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:08:53.884783Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:53.091609Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008027
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.595154+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Glycerol-Asparagine Agar (ISP Medium No.5)" to "glycerol_asparagine_agar_isp_medium_no_5" for consistent matching
2026-05-16T09:03:35.885094+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:06.550346+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.375904+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:59.138203+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:16.978661+00:00 recipe-merger Merged 3 duplicate recipes into canonical record Sources: TOGO_M52_Glycerol-Asparagine_Agar_ISP-5, acidic_glycerol_asparagine_agar_isp_medium_no_5, glycerol_asparagine_agar_isp_medium_no_5
2026-08-06T03:05:16.978710+00:00 merge_recipes.py MERGED_RECIPES merged 3 source recipe(s) on fingerprint 7258b29fb700b2fb64c708898106d744151d21f3ff3e77493afb8d59b62bdbe5