Overview
- External ontology
- TOGO:M2022 — AM3 + Nalidixic acid medium
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'am3_medium', 'source': 'TOGO', 'source_id': 'TOGO:M2021', 'original_category': 'bacterial'}
- {'name': 'am3_nalidixic_acid_kanamycin_medium', 'source': 'TOGO', 'source_id': 'TOGO:M2023', 'original_category': 'bacterial'}
- Notes
- Source: https://togomedium.org/medium/M2022 Original source: NBRC - NBRC_M1313 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1313
Composition
flowchart LR
CultureMech_008610["am3_nalidixic_acid_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008610 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008610 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_008610 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KH2PO4["KH2PO4"]
CultureMech_008610 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_K2HPO4["K2HPO4"]
CultureMech_008610 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Glucose["Glucose"]
CultureMech_008610 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Agar__if_needed_["Agar (if needed)"]
CultureMech_008610 --> ing6_Agar__if_needed_
ing7_Beef_extract["Beef extract"]
CultureMech_008610 --> ing7_Beef_extract
ing8_Peptone["Peptone"]
CultureMech_008610 --> ing8_Peptone
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 1.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
3.5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.32 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3.68 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 7 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 8 | Beef extract | 1.5 G_PER_L | Properties: Undefined component | |
| 9 | Peptone | 5 G_PER_L |
Solutions
Nalidixic acid solution (50 mg/ml)*
Dose: 1 G_PER_L
Properties: Solution, Defined component, Complex component
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- am3_medium (CultureMech:008609)
- am3_nalidixic_acid_kanamycin_medium (CultureMech:008611)
- am3_nalidixic_acid_medium (CultureMech:008610)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.894733Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2022 |
| 2026-01-28T19:48:35.365224Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:58.828120+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:58.636558Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008610 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.258688+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AM3 + Nalidixic acid medium" to "am3_nalidixic_acid_medium" for consistent matching |
| 2026-05-16T09:03:23.960023+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:48.353068+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.975331+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:22.824224+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: am3_medium, am3_nalidixic_acid_kanamycin_medium, am3_nalidixic_acid_medium |
| 2026-08-06T03:05:22.824264+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint df2061b3dec4d0b73bfdda0556709810ad336ff389cde1a67e6de1a06cc6837e |