Overview
- External ontology
- TOGO:M2450 — SP4-Z Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2450 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1076b.pdf
Composition
flowchart LR
CultureMech_009030["sp4_z_medium"]
ing0_distilled_water["distilled water"]
CultureMech_009030 --> ing0_distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_distilled_water -.-> CHEBI_15377
ing1_DNA__fish_sperm__SERVA_["DNA (fish sperm; SERVA)"]
CultureMech_009030 --> ing1_DNA__fish_sperm__SERVA_
ing2_Bacto_Peptone__BD_["Bacto-Peptone (BD)"]
CultureMech_009030 --> ing2_Bacto_Peptone__BD_
ing3_Tryptone["Tryptone"]
CultureMech_009030 --> ing3_Tryptone
ing4_PPLO_broth["PPLO broth"]
CultureMech_009030 --> ing4_PPLO_broth
ing5_Fetal_bovine_serum__heat_inactivated_["Fetal bovine serum (heat-inactivated)"]
CultureMech_009030 --> ing5_Fetal_bovine_serum__heat_inactivated_
ing6_Yeastolate__BD__2___autoclaved_["Yeastolate (BD; 2%, autoclaved)"]
CultureMech_009030 --> ing6_Yeastolate__BD__2___autoclaved_
ing7_Swine_serum__heat_inactivated_["Swine serum (heat-inactivated)"]
CultureMech_009030 --> ing7_Swine_serum__heat_inactivated_
ing8_CMRL_1066_medium__10x_concentrated__GIBCO_["CMRL-1066 medium (10x concentrated; GIBCO)"]
CultureMech_009030 --> ing8_CMRL_1066_medium__10x_concentrated__GIBCO_
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
distilled water
H2O 18.015 g/mol
|
130 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | DNA (fish sperm; SERVA) | 0.04 G_PER_L | Role: Growth factor | |
| 3 | Bacto-Peptone (BD) | 1 G_PER_L | Properties: Undefined component | |
| 4 | Tryptone | 2 G_PER_L | Properties: Undefined component | |
| 5 | PPLO broth | 0.7 G_PER_L | Properties: Undefined component, Complex component | |
| 6 | Fetal bovine serum (heat-inactivated) | 17 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 7 | Yeastolate (BD; 2%, autoclaved) | 20 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 8 | Swine serum (heat-inactivated) | 17 G_PER_L | Role: Nutrient source | |
| 9 | CMRL-1066 medium (10x concentrated; GIBCO) | 10 G_PER_L | Properties: Complex component, Defined component |
Solutions
Glucose (50% aqueous solution)
Dose: 2 G_PER_L
Role: Carbon source; Properties: Defined component, Organic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- TOGO_M2450_SP4-Z_Medium (CultureMech:009030)
- TOGO_M2451_SP4-Z_Medium (CultureMech:009031)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.515665Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2450 |
| 2026-01-28T19:48:16.058238Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:26.495846+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:02.046338Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009030 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.805258+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SP4-Z Medium" to "sp4_z_medium" for consistent matching |
| 2026-05-16T09:03:13.540165+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:36.811615+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:11.829052+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: TOGO_M2450_SP4-Z_Medium, TOGO_M2451_SP4-Z_Medium |
| 2026-08-06T03:05:11.829094+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 3cdaf57f397bc4a5bf990969c815ea139ffff5c379b8b592ff2a78e7b41196a4 |