Overview
- External ontology
- TOGO:M721 — MJY Medium For Pyrolinea Marinus
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M721 Original source: JCM - JCM_M700 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=700
Composition
flowchart LR
CultureMech_010129["mjy_medium_for_pyrolinea_marinus"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_010129 --> ing0_Yeast_extract
ing1_NH4Cl["NH4Cl"]
CultureMech_010129 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_NaNO3["NaNO3"]
CultureMech_010129 --> ing2_NaNO3
CHEBI_63005["CHEBI:63005"]
ing2_NaNO3 -.-> CHEBI_63005
ing3_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010129 --> ing3_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing3_Carbon_dioxide_gas -.-> CHEBI_16526
ing4_Nitrogen_gas["Nitrogen gas"]
CultureMech_010129 --> ing4_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing4_Nitrogen_gas -.-> CHEBI_17997
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound |
| 3 |
NaNO3
NO3.Na 84.994 g/mol
|
0.5 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 5 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
MJ(-N) synthetic seawater (see Medium [M260])
Dose: 1 G_PER_L
Cross-reference to Medium M260
8.0% NaHCO3 solution*
Dose: 5 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
5.0% Na2S・9H2O solution
Dose: 10 G_PER_L
Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- TOGO_M721_MJY_Medium_For_Pyrolinea_Marinus (CultureMech:010129)
- TOGO_M722_MJY_Medium_For_Pyrolinea_Marinus (CultureMech:010130)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.598368Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M721 |
| 2026-01-28T19:48:37.222908Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:05:02.098169+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:33.966326Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:11.544905Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010129 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.475693+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MJY Medium For Pyrolinea Marinus" to "mjy_medium_for_pyrolinea_marinus" for consistent matching |
| 2026-05-16T09:03:20.374211+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:43.888688+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.461776+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:18.733319+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: TOGO_M721_MJY_Medium_For_Pyrolinea_Marinus, TOGO_M722_MJY_Medium_For_Pyrolinea_Marinus |
| 2026-08-06T03:05:18.733369+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 039a40dfa24f45e6005472ac7612996ed75d708b46665bb58420efd222e5b3cb |