Overview
- External ontology
- UTEX:ag-diatom-medium (source link unavailable) — Ag Diatom Medium
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- UTEX prepares Ag Diatom Medium tube-by-tube from 1 cc CR1 Soil, 0.5 mg/12 mL MgCO3, and 12 mL Modified Bold 3N Medium.
Composition
flowchart LR
CultureMech_000023["ag_diatom_medium"]
ing0_CR1_Soil["CR1 Soil"]
CultureMech_000023 --> ing0_CR1_Soil
ing1_MgCO3["MgCO3"]
CultureMech_000023 --> ing1_MgCO3
CHEBI_31793["CHEBI:31793"]
ing1_MgCO3 -.-> CHEBI_31793
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | CR1 Soil | 1 cc per 15 mL tube VARIABLE | UTEX lists 1 cc CR1 Soil per 15 mL glass tube. | |
| 2 |
MgCO3
CO3.Mg 84.313 g/mol
MCIB CB486
|
0.5 MILLIMOLAR |
CHEBI:31793
magnesium carbonate
|
UTEX lists 0.5 mg MgCO3 per 12 mL liquid fill, for 0.5 mM final. |
Preparation
- Add CR1 Soil, MgCO3, and liquid medium in order to 15 mL glass tubes.
- Process the soil and liquid mixture within 6 hours; autoclave for 15 minutes for routine maintenance.
- Allow tubes to gas-equilibrate for 2 days before use.
Solutions
Modified Bold 3N Medium
Dose: 1000 ML_PER_L
Converted from 12 mL Modified Bold 3N Medium per 12 mL liquid fill.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | utex-import | Imported from UTEX Culture Collection | Source ID: ag-diatom-medium, URL: https://utex.org/products/ag-diatom-medium |
| 2026-03-13T06:18:52.907046Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000023 |
| 2026-03-17T19:45:03.877188+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ag Diatom Medium" to "ag_diatom_medium" for consistent matching |
| 2026-05-16T09:02:55.821480+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1 |
| 2026-05-16T09:11:01.028101+00:00 | migrate_preparation_steps.py | MIGRATED_PREPARATION_STEPS | instruction->action+description for 9 step(s) |
| 2026-08-30T00:58:30.569974+00:00 | repair_utex_ingredients.py | REPAIRED_UTEX_COMPOSITION | Restored 3 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779. |
| 2026-09-06T00:00:00-07:00 | repair_utex_cr1_diatom_score35.py | RESOLVED_UTEX_CR1_DIATOM_SCORE35 | UTEX prepares Ag Diatom Medium tube-by-tube from 1 cc CR1 Soil, 0.5 mg/12 mL MgCO3, and 12 mL Modified Bold 3N Medium. |