bg_11_1_nacl_medium (CultureMech:000027)

Overview

External ontology

UTEX:bg-11-plus-1-percent-nacl-medium (source link unavailable) — BG-11 + 1% NaCl Medium

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

UTEX prepares this BG-11 variant by supplementing the base with 10 g/L NaCl.

Composition

flowchart LR
    CultureMech_000027["bg_11_1_nacl_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000027 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710

Ingredients (1)

#	Ingredient	Concentration	Ontology	Notes
1	NaCl Cl.Na 58.443 g/mol Fisher S271-500	0.17 MOLAR	CHEBI:26710 sodium chloride	UTEX separately prints 10 g/L and 0.17 M NaCl. Molarity is retained as a source assertion, not an exact calculated equivalence.

Preparation

Prepare BG-11 Medium through its first mixing step.
Add 10 g/L NaCl and bring the total volume to 1 L with dH2O.
Cover and autoclave the medium.
Store at refrigerator temperature.

Solutions

BG-11 Medium

CultureMech:000029

Recipe base, not a measured 1000 mL/L prepared-medium input. UTEX labels BG-11 as 1 L in the table but stops its preparation after step one; NaCl is added before liquid-medium makeup to a final 1 L. No fixed prepared-base dose is established.

Target organisms

No target organisms are recorded.

Recipe variants

Name
Optional agar formulation
Relationship
PHYSICAL_STATE_VARIANT
Description
Source-described two-portion preparation; sodium-thiosulfate dose remains unresolved.
Modifications
- For the agar alternative, perform BG-11 preparation step one, add the formulation-specific NaCl, and make this portion up to 500 mL with dH2O.
- In another container, add 15 g agar to 500 mL dH2O. Autoclave both preparations separately, then cool them to 45-50 degrees C in a water bath.
- Mix sterile sodium thiosulfate into the agar preparation before combining both portions. The salt-variant page does not specify a thiosulfate dose; no amount is inferred from the linked base recipe. Mix promptly, allow to cool and refrigerate.
Evidence
- Reference
  https://utex.org/products/bg-11-plus-1-percent-nacl-medium
  Supports
  SUPPORT
  Snippet
  15 g
  Explanation
  variants[0].modifications: Agar Directions 2-3 specify two portions involving "500 mL"; the separate agar amount is "15 g". Directions 4-8 describe separate autoclaving, cooling to "45-50" degrees C, addition of sterile "Sodium Thiosulfate", combination, cooling and refrigeration. The whole agar subsection was inspected and contains no local thiosulfate amount. The 15 g is a batch addition; final volume after sterile supplement is not measured here.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	utex-import	Imported from UTEX Culture Collection	Source ID: bg-11-plus-1-percent-nacl-medium, URL: https://utex.org/products/bg-11-plus-1-percent-nacl-medium
2026-03-13T06:18:52.930808Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000027
2026-03-17T19:45:03.897099+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "BG-11 + 1% NaCl Medium" to "bg_11_1_nacl_medium" for consistent matching
2026-05-16T09:02:56.370827+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.533420+00:00	migrate_preparation_steps.py	MIGRATED_PREPARATION_STEPS	instruction->action+description for 9 step(s)
2026-08-30T00:58:31.292090+00:00	repair_utex_ingredients.py	REPAIRED_UTEX_COMPOSITION	Restored 2 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-05T00:00:00-07:00	repair_utex_bg11_spirulina.py	RESOLVED_UTEX_BG11_SPIRULINA_GRAPH	UTEX prepares this BG-11 variant by supplementing the base with 10 g/L NaCl.
2026-10-11T02:43:27.765962+00:00	codex	CURATED_UTEX_VOLVOCACEAN_BG11_CONCENTRATION_EVIDENCE	Retained source-printed NaCl molarity with evidence; removed the unsupported prepared-BG-11 dose, clarified salt addition before volume makeup, and recorded the optional agar procedure separately. The thiosulfate dose remains unresolved. No taxa-media associations added.