botryococcus_medium

CultureMech:000035 algae COMPLEX LIQUID
Original name: Botryococcus Medium

Overview

External ontology
UTEX:botryococcus-medium (source link unavailable) — Botryococcus Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX adds 1 mL of a 2 g/100 mL diammonium hydrogen phosphate stock to 940 mL prepared Bristol Medium and makes the final volume up to 1 L. The source reports 0.151 mM as the final phosphate contribution, not stock strength.

Composition

flowchart LR
    CultureMech_000035["botryococcus_medium"]
    ing0__NH4_2HPO4["(NH4)2HPO4"]
    CultureMech_000035 --> ing0__NH4_2HPO4
    CHEBI_63051["CHEBI:63051"]
    ing0__NH4_2HPO4 -.-> CHEBI_63051
    ing1_dH2O["dH2O"]
    CultureMech_000035 --> ing1_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing1_dH2O -.-> CHEBI_15377

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 (NH4)2HPO4
2H4N.HO4P 132.056 g/mol
Fisher A686
0.151 MILLIMOLAR CHEBI:63051
diammonium hydrogen phosphate
 UTEX lists 1 mL/L of a 2 g/100 mL stock, for 0.151 mM final.
2 dH2O
H2O 18.015 g/mol
CHEBI:15377
water
 Added to bring 940 mL Bristol Medium and 1 mL stock to 1 L.

Preparation

  1. Prepare 940 mL of Bristol Medium.
  2. Add 1 mL/L (NH4)2HPO4 stock.
  3. Bring the total volume to 1 L with dH2O.
  4. Cover the flask and autoclave to sterilize.

Solutions

Bristol Medium

Dose: 940 ML_PER_L

CultureMech:000037

UTEX lists 940 mL prepared Bristol Medium per 1 L final medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: botryococcus-medium, URL: https://utex.org/products/botryococcus-medium
2026-03-13T06:18:52.979553Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000035
2026-03-17T19:45:03.938630+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Botryococcus Medium" to "botryococcus_medium" for consistent matching
2026-05-16T09:02:55.867642+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.079169+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.640980+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 2 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_algal_blends.py RESOLVED_UTEX_ALGAL_BLEND_GRAPH UTEX prepares Botryococcus Medium by supplementing prepared Bristol Medium with 0.151 mM diammonium hydrogen phosphate stock and bringing the final volume to 1 L with dH2O.
2026-10-10T03:53:28.828041+00:00 codex CURATED_UTEX_BRISTOL_BBM_INGREDIENT_AND_PREPARATION_EVIDENCE The BBM trace source prints MnCl with tetrahydrate; its intended chemical identity and the existing MnCl2 amount remain unverified. Added node evidence supports only that source-spelling conflict. BBM trace concentration columns and stock-page parenthetical micromolar values conflict with explicit stock preparation amounts. Preserve inspected Amount-per-litre stock recipes, not those incompatible columns; no new final-medium conversions. The BBM iron stock does not specify acid strength. No density, purity or molarity is assumed. The BBM study cites Stein 1973; exact equivalence with the current UTEX parent is unverified. NH4Cl ppm basis and culture working volume remain unspecified; no numerical conversion or aliquot inference. Bristol-NaCl uses an unfinished parent preparation; its table one-litre row is not certified as a working dose of completed Bristol. The reference and explicit stage notes are retained without a fabricated dose. Optional agar formulations are not silently added to these liquid records. No new taxa-media associations, IDs or growth/role assertions are curated. Supplier-catalog identities, generic incubation/light claims and unrelated preexisting organism evidence are not blanket-certified by this ingredient review.