bristol_nacl_medium

CultureMech:000036 algae COMPLEX LIQUID
Original name: Bristol-NaCl Medium

Overview

External ontology
UTEX:bristol-nacl-medium (source link unavailable) — Bristol-NaCl Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX adds 3.6 g NaCl to the Bristol mineral preparation before final volume adjustment to 1 L, rather than to a one-litre aliquot of finished Bristol Medium.

Composition

flowchart LR
    CultureMech_000036["bristol_nacl_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000036 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
Fisher S271-500
0.062 MOLAR CHEBI:26710
sodium chloride
 UTEX lists 3.6 g/L NaCl, equivalent to 0.062 M.

Preparation

  1. Prepare Bristol Medium through step one.
  2. Add NaCl and bring the total volume to 1 L with dH2O.
  3. Cover and autoclave the medium.
  4. Store at refrigerator temperature.

Solutions

Bristol mineral base (before volume adjustment)

CultureMech:000037

Use Bristol mineral preparation through step one at the one-litre recipe scale; add 3.6 g NaCl before making the child up to 1 L. This is not a one-litre aliquot of completed Bristol Medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: bristol-nacl-medium, URL: https://utex.org/products/bristol-nacl-medium
2026-03-13T06:18:52.983755Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000036
2026-03-17T19:45:03.942375+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Bristol-NaCl Medium" to "bristol_nacl_medium" for consistent matching
2026-05-16T09:02:56.399466+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.561056+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:31.387479+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 2 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_remaining_score50.py RESOLVED_UTEX_SCORE50_ALGAE_GRAPH UTEX prepares Bristol-NaCl Medium by adding 3.6 g/L NaCl to prepared Bristol Medium and bringing the total volume to 1 L.
2026-10-10T03:53:28.828041+00:00 codex CURATED_UTEX_BRISTOL_BBM_INGREDIENT_AND_PREPARATION_EVIDENCE The BBM trace source prints MnCl with tetrahydrate; its intended chemical identity and the existing MnCl2 amount remain unverified. Added node evidence supports only that source-spelling conflict. BBM trace concentration columns and stock-page parenthetical micromolar values conflict with explicit stock preparation amounts. Preserve inspected Amount-per-litre stock recipes, not those incompatible columns; no new final-medium conversions. The BBM iron stock does not specify acid strength. No density, purity or molarity is assumed. The BBM study cites Stein 1973; exact equivalence with the current UTEX parent is unverified. NH4Cl ppm basis and culture working volume remain unspecified; no numerical conversion or aliquot inference. Bristol-NaCl uses an unfinished parent preparation; its table one-litre row is not certified as a working dose of completed Bristol. The reference and explicit stage notes are retained without a fabricated dose. Optional agar formulations are not silently added to these liquid records. No new taxa-media associations, IDs or growth/role assertions are curated. Supplier-catalog identities, generic incubation/light claims and unrelated preexisting organism evidence are not blanket-certified by this ingredient review.