ajs

CultureMech:000041 algae COMPLEX LIQUID
Original name: AJS

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_AJS.pdf

Composition

flowchart LR
    CultureMech_000041["ajs"]
    ing0_Concentrated_H2SO4["Concentrated H2SO4"]
    CultureMech_000041 --> ing0_Concentrated_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing0_Concentrated_H2SO4 -.-> CHEBI_26836

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 Concentrated H2SO4
H2O4S 98.079 g/mol
10 ML_PER_L CHEBI:26836
sulfuric acid
Source-asserted addition to approximately 1 L final medium.

Preparation

  1. Mix 970 mL JM with 30 mL SE2 per approximately 1 L final medium.
  2. Add 10 mL concentrated H2SO4 to obtain approximately pH 1.5.
  3. Autoclave at 15 psi for 15 minutes.

Solutions

JM (Jaworski's Medium)

Dose: 970 ML_PER_L

CultureMech:000074

97% constituent medium. Source: CCAP Medium MR_AJS.pdf.

SE2 (Soil Extract 2)

Dose: 30 ML_PER_L

CultureMech:000129

3% constituent medium. Source: CCAP Medium MR_AJS.pdf.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 ccap-import Imported from CCAP Culture Collection Source ID: AJS, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_AJS.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.447422 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.000473Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000041
2026-03-17T19:45:03.957523+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AJS" to "ajs" for consistent matching
2026-04-04T12:54:55.204775 batch_process_collection_media EXPANDED_INGREDIENTS
2026-05-16T08:57:02.060779+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(4 keys) -> list(3 entries)
2026-05-16T09:02:56.200871+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-25T00:00:00-07:00 repair_ccap_instruction_rows.py REPAIRED_CCAP_INSTRUCTION_ROWS Repaired preparation text and nested-recipe flattening against CCAP MR_AJS.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable.
2026-09-13T00:00:00-07:00 repair_ccap_sources_score10.py NORMALIZED_CCAP_SOURCE_SCORE10 Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.