Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_AJS.pdf
Composition
flowchart LR
CultureMech_000041["ajs"]
ing0_Concentrated_H2SO4["Concentrated H2SO4"]
CultureMech_000041 --> ing0_Concentrated_H2SO4
CHEBI_26836["CHEBI:26836"]
ing0_Concentrated_H2SO4 -.-> CHEBI_26836
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Concentrated H2SO4
H2O4S 98.079 g/mol
|
10 ML_PER_L |
CHEBI:26836
sulfuric acid
|
Source-asserted addition to approximately 1 L final medium. |
Preparation
- Mix 970 mL JM with 30 mL SE2 per approximately 1 L final medium.
- Add 10 mL concentrated H2SO4 to obtain approximately pH 1.5.
- Autoclave at 15 psi for 15 minutes.
Solutions
JM (Jaworski's Medium)
Dose: 970 ML_PER_L
97% constituent medium. Source: CCAP Medium MR_AJS.pdf.
SE2 (Soil Extract 2)
Dose: 30 ML_PER_L
3% constituent medium. Source: CCAP Medium MR_AJS.pdf.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: AJS, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_AJS.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.447422 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.000473Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000041 |
| 2026-03-17T19:45:03.957523+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AJS" to "ajs" for consistent matching |
| 2026-04-04T12:54:55.204775 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.060779+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.200871+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-25T00:00:00-07:00 | repair_ccap_instruction_rows.py | REPAIRED_CCAP_INSTRUCTION_ROWS | Repaired preparation text and nested-recipe flattening against CCAP MR_AJS.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |