combo_diatom_medium (CultureMech:000156)

Overview

External ontology

UTEX:combo-diatom-medium (source link unavailable) — COMBO Diatom Medium

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

UTEX prepares COMBO Diatom Medium tube-by-tube from 1 cc CR1 Soil, 0.5 mg/12 mL MgCO3, and 12 mL Modified COMBO Medium.

Composition

flowchart LR
    CultureMech_000156["combo_diatom_medium"]
    ing0_CR1_Soil["CR1 Soil"]
    CultureMech_000156 --> ing0_CR1_Soil
    ing1_MgCO3["MgCO3"]
    CultureMech_000156 --> ing1_MgCO3
    CHEBI_31793["CHEBI:31793"]
    ing1_MgCO3 -.-> CHEBI_31793

Ingredients (2)

#	Ingredient	Concentration	Ontology	Notes
1	CR1 Soil	1 cc per 15 mL tube VARIABLE		UTEX lists 1 cc CR1 Soil per 15 mL glass tube.
2	MgCO3 CO3.Mg 84.313 g/mol MCIB CB486	0.5 MILLIMOLAR	CHEBI:31793 magnesium carbonate	UTEX lists 0.5 mg MgCO3 per 12 mL liquid fill, for 0.5 mM final.

Preparation

Add CR1 Soil, MgCO3, and Modified COMBO Medium in order to 15 mL glass tubes.
Within 6 hours of mixing soil and liquid, pasteurize the tubes by heating them to just over 98 C for 2 hours.
Allow the tubes to cool to room temperature for 20 to 24 hours, then repeat the pasteurization cycle for 3 successive days.
For routine maintenance and transfer, autoclave the tubes for 15 minutes instead of pasteurizing them.
Allow pasteurized or autoclaved tubes to gas-equilibrate for 2 days before use.

Solutions

Modified COMBO Medium

Dose: 1000 ML_PER_L

CultureMech:000181

Converted from 12 mL Modified COMBO Medium per 12 mL liquid fill.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	utex-import	Imported from UTEX Culture Collection	Source ID: combo-diatom-medium, URL: https://utex.org/products/combo-diatom-medium
2026-03-13T06:18:53.324103Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000156
2026-03-17T19:45:04.267793+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Combo Diatom Medium" to "combo_diatom_medium" for consistent matching
2026-05-16T09:02:55.940700+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.135447+00:00	migrate_preparation_steps.py	MIGRATED_PREPARATION_STEPS	instruction->action+description for 9 step(s)
2026-08-30T00:58:30.717792+00:00	repair_utex_ingredients.py	REPAIRED_UTEX_COMPOSITION	Restored 3 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00	repair_utex_combo_diatom_score35.py	RESOLVED_UTEX_COMBO_DIATOM_SCORE35	UTEX prepares COMBO Diatom Medium tube-by-tube from 1 cc CR1 Soil, 0.5 mg/12 mL MgCO3, and 12 mL Modified COMBO Medium.