soil_extract_sodium_metasilicate_medium (CultureMech:000222)

Overview

External ontology

UTEX:soil-extract-plus-sodium-metasilicate-medium (source link unavailable) — Soil Extract + Sodium Metasilicate Medium

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

UTEX prepares Soil Extract + Sodium Metasilicate Medium by replacing 40 mL of a 1 L Bristol Medium batch with 40 mL filtered Soilwater: GR+ Medium supernatant, autoclaving, and then adding filter-sterilized sodium metasilicate.

Composition

flowchart LR
    CultureMech_000222["soil_extract_sodium_metasilicate_medium"]
    ing0_Sodium_Metasilicate["Sodium Metasilicate"]
    CultureMech_000222 --> ing0_Sodium_Metasilicate
    CHEBI_60720["CHEBI:60720"]
    ing0_Sodium_Metasilicate -.-> CHEBI_60720

Ingredients (1)

#	Ingredient	Concentration	Ontology	Notes
1	Sodium Metasilicate 2Na.O3Si 122.063 g/mol	200 MICROMOLAR	CHEBI:60720 sodium silicate	UTEX adds 1 mL of 200 mM sodium metasilicate per liter.

Preparation

Prepare 1 L of Bristol Medium and discard 40 mL.
Filter prepared Soilwater: GR+ Medium to remove soil particles.
Add 40 mL of Soilwater: GR+ Medium supernatant to the remaining 960 mL Bristol Medium.
Cover and autoclave the medium.
When cooled, add filter-sterilized sodium metasilicate.
Store at refrigerator temperature.

Solutions

Bristol Medium

Dose: 960 ML_PER_L

CultureMech:000037

UTEX lists 960 mL prepared Bristol Medium per 1 L final medium.

Soilwater: GR+ Medium

Dose: 40 ML_PER_L

CultureMech:000225

UTEX lists 40 mL filtered Soilwater: GR+ Medium supernatant.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	utex-import	Imported from UTEX Culture Collection	Source ID: soil-extract-plus-sodium-metasilicate-medium, URL: https://utex.org/products/soil-extract-plus-sodium-metasilicate-medium
2026-03-13T06:18:53.604809Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000222
2026-03-17T19:45:04.523717+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Soil Extract + Sodium Metasilicate Medium" to "soil_extract_sodium_metasilicate_medium" for consistent matching
2026-05-16T09:02:57.002074+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.953141+00:00	migrate_preparation_steps.py	MIGRATED_PREPARATION_STEPS	instruction->action+description for 9 step(s)
2026-08-30T00:58:32.134785+00:00	repair_utex_ingredients.py	REPAIRED_UTEX_COMPOSITION	Restored 3 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-07T00:00:00-07:00	repair_utex_a_bg11_score30.py	RESOLVED_UTEX_A_BG11_SCORE30	UTEX prepares Soil Extract + Sodium Metasilicate Medium by replacing 40 mL of a 1 L Bristol Medium batch with 40 mL filtered Soilwater: GR+ Medium supernatant, autoclaving, and then adding filter-sterilized sodium metasilicate.