trebouxia_medium (CultureMech:000234)

Overview

External ontology

UTEX:trebouxia-medium (source link unavailable) — Trebouxia Medium

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

UTEX prepares Trebouxia Medium by supplementing Bristol Medium with prepared Soilwater: GR+ Medium, Proteose Peptone, and glucose before autoclaving.

Composition

flowchart LR
    CultureMech_000234["trebouxia_medium"]
    ing0_Proteose_Peptone["Proteose Peptone"]
    CultureMech_000234 --> ing0_Proteose_Peptone
    ing1_Glucose["Glucose"]
    CultureMech_000234 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234

Ingredients (2)

#	Ingredient	Concentration	Ontology	Notes
1	Proteose Peptone BD 211684	10 G_PER_L	MICRO:0000180 (source link unavailable) Proteose Peptone	UTEX lists 10 g/L Proteose Peptone, BD 211684.
2	Glucose C6H12O6 180.156 g/mol	0.11 MOLAR	CHEBI:17234 glucose	UTEX lists 20 g/L Glucose, with BACTO-dextrose or Sigma D 9634 as the source for 0.11 M final.

Preparation

Prepare 1 L of Bristol Medium, transfer approximately 825 mL to a 2 L Erlenmeyer flask, and save the remaining Bristol Medium.
To the approximately 825 mL Bristol Medium, add the listed components in order while stirring continuously.
Bring the total volume to 1 L with the reserved Bristol Medium.
Cover and autoclave the medium.
Store at refrigerator temperature.

Solutions

Bristol Medium

CultureMech:000037

Start with approximately 825 mL prepared Bristol Medium, then use additional reserved Bristol Medium to bring the supplemented recipe to 1 L. The combined initial and makeup dose is not quantified; 825 mL/L is not the total dose.

Soilwater: GR+ Medium

Dose: 140 ML_PER_L

CultureMech:000225

UTEX lists 140 mL prepared Soilwater: GR+ Medium per liter.

Target organisms

No target organisms are recorded.

Recipe variants

Name
Trebouxia Medium with agar
Relationship
SUPPLEMENTED_VARIANT
Description
Optional source-listed agar formulation; the parent remains liquid.
Modifications
- After making up the liquid recipe to 1 L with Bristol Medium, add 15 g agar; do not mix.
Purpose
Preserve the separately specified solid formulation
Evidence
- Reference
  https://utex.org/products/trebouxia-medium
  Supports
  SUPPORT
  Snippet
  For 1.5% (w/v) agar medium: add 15 g of agar into the flask; do not mix.
  Explanation
  Supports variants[0].modifications only. The source labels this the 1.5% agar formulation; no new measured final volume after agar addition is claimed. No agar is added to the liquid parent.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	utex-import	Imported from UTEX Culture Collection	Source ID: trebouxia-medium, URL: https://utex.org/products/trebouxia-medium
2026-03-13T06:18:53.663553Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000234
2026-03-17T19:45:04.573294+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Trebouxia Medium" to "trebouxia_medium" for consistent matching
2026-05-16T09:02:56.152033+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.369068+00:00	migrate_preparation_steps.py	MIGRATED_PREPARATION_STEPS	instruction->action+description for 9 step(s)
2026-08-30T00:58:31.034673+00:00	repair_utex_ingredients.py	REPAIRED_UTEX_COMPOSITION	Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00	repair_utex_organic_score35.py	RESOLVED_UTEX_ORGANIC_SCORE35	UTEX prepares Trebouxia Medium by supplementing Bristol Medium with prepared Soilwater: GR+ Medium, Proteose Peptone, and glucose before autoclaving.
2026-10-10T21:08:21.569593+00:00	codex	CURATED_UTEX_ORGANIC_MEDIUM_INPUT_BASES_AND_CONCENTRATION_EVIDENCE	Exact source-URL and snippet evidence for input doses, optional agar and retained concentrations. Chemical identity mappings and unrelated growth/role claims are unchanged, not independently certified. No taxa-media association added.