Overview
- External ontology
- UTEX:volvox-dextrose-medium (source link unavailable) — Volvox-Dextrose Medium
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- UTEX prepares Volvox-Dextrose Medium as a glucose-amended Volvox Medium recipe with Vitamin B12 and Biotin Vitamin Solution added after autoclaving.
Composition
flowchart LR
CultureMech_000237["volvox_dextrose_medium"]
ing0_Glucose["Glucose"]
CultureMech_000237 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
0.06 MOLAR |
CHEBI:17234
glucose
|
UTEX adds 10 g/L glucose after all other Volvox Medium stock solutions, with BACTO-dextrose or Sigma D 9634 as the source. |
Preparation
- Prepare Volvox Medium, but add 10 g glucose after all other stock solutions have been added.
- Bring the total volume to 1 L with dH2O.
- Cover and autoclave the medium.
- Allow the medium to cool, then add sterile vitamins.
Solutions
Volvox Medium
Dose: 1 L per 1 L final VARIABLE
UTEX prepares the recipe as Volvox Medium modified with 10 g/L glucose added after all other stock solutions.
Vitamin B12
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | 1000 ML_PER_L | UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer. |
| HEPES buffer pH 7.8 | 12 G_PER_L | Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock. |
| Vitamin B12 | 0.135 G_PER_L | Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock. |
UTEX adds 1 mL Vitamin B12 stock per liter.
Biotin Vitamin Solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | 1000 ML_PER_L | UTEX prepares the biotin stock in 200 mL HEPES buffer. |
| HEPES buffer pH 7.8 | 12 G_PER_L | Converted from 2.4 g/200 mL in the UTEX biotin stock. |
| Biotin | 0.025 G_PER_L | Converted from 0.005 g/200 mL in the UTEX biotin stock. |
UTEX adds 1 mL Biotin Vitamin Solution per liter.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | utex-import | Imported from UTEX Culture Collection | Source ID: volvox-dextrose-medium, URL: https://utex.org/products/volvox-dextrose-medium |
| 2026-03-13T06:18:53.678255Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000237 |
| 2026-03-17T19:45:04.586169+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Volvox-Dextrose Medium" to "volvox_dextrose_medium" for consistent matching |
| 2026-05-16T09:02:57.101209+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1 |
| 2026-05-16T09:11:02.031193+00:00 | migrate_preparation_steps.py | MIGRATED_PREPARATION_STEPS | instruction->action+description for 9 step(s) |
| 2026-08-30T00:58:32.336297+00:00 | repair_utex_ingredients.py | REPAIRED_UTEX_COMPOSITION | Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779. |
| 2026-09-06T00:00:00-07:00 | repair_utex_volvox_p49_score35.py | RESOLVED_UTEX_VOLVOX_P49_SCORE35 | UTEX prepares Volvox-Dextrose Medium as a glucose-amended Volvox Medium recipe with Vitamin B12 and Biotin Vitamin Solution added after autoclaving. |