Overview
- External ontology
- mediadive.medium:J530 (source link unavailable) — MODIFIED BALCH'S METHANOGENS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=530
Composition
flowchart LR
CultureMech_000300["modified_balchs_methanogens_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_000300 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_000300 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_000300 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_NaCl["NaCl"]
CultureMech_000300 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000300 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000300 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000300 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Yeast_extract["Yeast extract"]
CultureMech_000300 --> ing7_Yeast_extract
ing8_Trypticase_peptone["Trypticase peptone"]
CultureMech_000300 --> ing8_Trypticase_peptone
ing9_Sodium_acetate["Sodium acetate"]
CultureMech_000300 --> ing9_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Sodium_acetate -.-> CHEBI_32954
ing10_Sodium_formate["Sodium formate"]
CultureMech_000300 --> ing10_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing10_Sodium_formate -.-> CHEBI_62965
ing11_NaHCO3["NaHCO3"]
CultureMech_000300 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_000300 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_000300 --> ing13_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing13_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing14_Resazurin["Resazurin"]
CultureMech_000300 --> ing14_Resazurin
CHEBI_8806["CHEBI:8806"]
ing14_Resazurin -.-> CHEBI_8806
ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_000300 --> ing15_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
ing16_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_000300 --> ing16_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing16_MnSO4_x_n_H2O -.-> CHEBI_86360
ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_000300 --> ing17_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000300 --> ing18_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000300 --> ing19_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
ing20_AlK_SO4_2["AlK(SO4)2"]
CultureMech_000300 --> ing20_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing20_AlK_SO4_2 -.-> CHEBI_86463
ing21_H3BO3["H3BO3"]
CultureMech_000300 --> ing21_H3BO3
CHEBI_33118["CHEBI:33118"]
ing21_H3BO3 -.-> CHEBI_33118
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000300 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_Biotin["Biotin"]
CultureMech_000300 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_000300 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000300 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_000300 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_Riboflavin["Riboflavin"]
CultureMech_000300 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_000300 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_000300 --> ing29_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_pantothenate -.-> CHEBI_31345
CultureMech_000300 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.294118 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Copied from referenced JCM Medium 242 |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.294118 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Copied from referenced JCM Medium 242 |
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.294118 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Copied from referenced JCM Medium 242 |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
1.588235 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.588235, 1.0] | Copied from JCM Medium 242 |
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.127451 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.127451, 3.0] | Copied from JCM Medium 242 |
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.10196078 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.00196078, 0.1] | Copied from JCM Medium 242 |
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.10784314 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.00784314, 0.1] | Copied from JCM Medium 242 |
| 8 | Yeast extract | 1.96078 G_PER_L | Copied from referenced JCM Medium 242 | |
| 9 | Trypticase peptone | 1.96078 G_PER_L | Copied from referenced JCM Medium 242 | |
| 10 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
2.45098 G_PER_L |
CHEBI:32954
sodium acetate
|
Copied from referenced JCM Medium 242 |
| 11 |
Sodium formate
CHO2.Na 68.007 g/mol
|
2.45098 G_PER_L |
CHEBI:62965
sodium formate
|
Copied from referenced JCM Medium 242 |
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4.90196 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Copied from referenced JCM Medium 242 |
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.490196 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Copied from referenced JCM Medium 242 |
| 14 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.490196 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Copied from referenced JCM Medium 242 |
| 15 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000980392 G_PER_L |
CHEBI:8806
Resazurin
|
Copied from referenced JCM Medium 242 |
| 16 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Copied from referenced JCM Medium 242 |
| 17 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Copied from referenced JCM Medium 242 |
| 18 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Copied from referenced JCM Medium 242 |
| 19 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Copied from referenced JCM Medium 242 |
| 20 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Copied from referenced JCM Medium 242 |
| 21 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
Copied from referenced JCM Medium 242 |
| 22 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Copied from referenced JCM Medium 242 |
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Copied from referenced JCM Medium 242 |
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
Copied from referenced JCM Medium 242 |
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
Copied from referenced JCM Medium 242 |
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Copied from referenced JCM Medium 242 |
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Copied from referenced JCM Medium 242 |
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
Copied from referenced JCM Medium 242 |
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
Copied from referenced JCM Medium 242 |
| 30 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Copied from referenced JCM Medium 242 |
| 31 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
Copied from referenced JCM Medium 242 |
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Copied from referenced JCM Medium 242 |
| 33 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Copied from referenced JCM Medium 242 |
Preparation
- Use Medium No. 242 supplemented with 20 ml/L Fatty acid mixture (see Medium No. 266) and 1 ml/L coenzyme M solution (0.01% 2-mercaptoethanesulfonic acid, filter-sterilized).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.852309Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J530 |
| 2026-02-03T11:26:44.154921 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:54.280945Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000300 |
| 2026-03-17T19:45:05.240081+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED BALCH'S METHANOGENS MEDIUM" to "modified_balchs_methanogens_medium" for consistent matching |
| 2026-04-04T18:49:19.282965 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from jcm_medium_no_242 (CultureMech:002603) |
| 2026-05-16T08:57:03.267787+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-05-16T09:02:57.717404+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:15.010460+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.348750+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |