Overview
- External ontology
- mediadive.medium:956 (source link unavailable) — MEDIUM FOR DSM 14457 AND 14458
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium956.pdf DSMZ 956 defines mutually exclusive methanol and methylamine alternatives. This record retains their common base and both options in variants; choose exactly one, not both. The unsupplemented base is not asserted to support growth. The methylamine-specific record is CultureMech:006899.
Ingredients (0)
No ingredients recorded.
Preparation
- pH 7.2. To the sterilised medium add sterile methanol (to a final concentration of 0.5%) or methylamine (to a final concentration of 0.3%).
Solutions
DSMZ 956 base preparation
| Ingredient | Concentration | Notes |
|---|---|---|
| KH2PO4 | Source batch input: 2.000 g; KH2PO4 2.000 g The recipe starts with 1000 ml distilled water before supplementation. No prepared-base or final-medium mass concentration is asserted. | |
| (NH4)2SO4 | Source batch input: 2.000 g; (NH4)2SO4 2.000 g The recipe starts with 1000 ml distilled water before supplementation. No prepared-base or final-medium mass concentration is asserted. | |
| MgSO4 x 7 H2O | Source batch input: 0.125 g; MgSO4 x 7 H2O 0.125 g The recipe starts with 1000 ml distilled water before supplementation. No prepared-base or final-medium mass concentration is asserted. | |
| NaCl | Source batch input: 0.500 g; NaCl 0.500 g The recipe starts with 1000 ml distilled water before supplementation. No prepared-base or final-medium mass concentration is asserted. | |
| FeSO4 x 7 H2O | Source batch input: 0.002 g; FeSO4 x 7 H2O 0.002 g The recipe starts with 1000 ml distilled water before supplementation. No prepared-base or final-medium mass concentration is asserted. | |
| Distilled water | 1 L | Starting-water batch amount: 1000 ml / 1000 ml per L = 1 L. Not a final-volume make-up instruction or a mass concentration. |
Curator-described preparation stage, not a separately accessioned stock. Composition records source batch inputs. The 1000 ml water is an input, not an explicit prepared-base or final-medium volume; no absolute base dose is inferred.
Target organisms
No target organisms are recorded.
Recipe variants
- Name
- Methanol alternative
- Relationship
- SUPPLEMENTED_VARIANT
- Description
- One of two mutually exclusive source-supported supplements to the common base; not an assertion that the unsupplemented base supports growth.
- Modifications
- After sterilisation add sterile methanol to a final concentration of 0.5% (percentage basis unspecified).
- Use this alternative instead of methylamine; do not add both.
- Purpose
- Select one supplementation branch of the original DSMZ 956 recipe.
- Evidence
- Reference
- https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium956.pdf
- Supports
- SUPPORT
- Snippet
- pH 7.2. To the sterilised medium add sterile methanol (to a final concentration of 0.5%) or methylamine (to a final concentration of 0.3%).
- Explanation
- Moves Methanol from the apparent combined ingredient list to variants[0]. Withdraws the unsupported 5 g/L encoding. The source percent has no defined basis, so no numeric percent or mass concentration slot is added. The removed node's ontology annotations remain in the append-only change event, not as a new grounding claim. Original DSMZ 956, PDF page 1; capture SHA256=156fe7f9a826c658bf690c9c892ba7d137848d397f55cf93568c4c3145374f39; accessed_on=2026-10-07. Display whitespace normalized; source quantities and chemical forms checked visually. Ontology annotations are preserved where their ingredient remains, not independently re-grounded.
- Name
- Methylamine alternative
- Relationship
- SUPPLEMENTED_VARIANT
- Description
- One of two mutually exclusive source-supported supplements to the common base; not an assertion that the unsupplemented base supports growth. The separately accessioned methylamine branch is CultureMech:006899.
- Modifications
- After sterilisation add sterile methylamine to a final concentration of 0.3% (percentage basis unspecified).
- Use this alternative instead of methanol; do not add both.
- Purpose
- Select one supplementation branch of the original DSMZ 956 recipe.
- Evidence
- Reference
- https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium956.pdf
- Supports
- SUPPORT
- Snippet
- pH 7.2. To the sterilised medium add sterile methanol (to a final concentration of 0.5%) or methylamine (to a final concentration of 0.3%).
- Explanation
- Moves Methylamine from the apparent combined ingredient list to variants[1]. Withdraws the unsupported 3 g/L encoding. The source percent has no defined basis, so no numeric percent or mass concentration slot is added. The removed node's ontology annotations remain in the append-only change event, not as a new grounding claim. Original DSMZ 956, PDF page 1; capture SHA256=156fe7f9a826c658bf690c9c892ba7d137848d397f55cf93568c4c3145374f39; accessed_on=2026-10-07. Display whitespace normalized; source quantities and chemical forms checked visually. Ontology annotations are preserved where their ingredient remains, not independently re-grounded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.889918Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 956 |
| 2026-03-13T06:19:10.620584Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002134 |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.312378+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM FOR DSM 14457 AND 14458" to "medium_for_dsm_14457_and_14458" for consistent matching |
| 2026-06-05T07:41:29.011762+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.039272+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |
| 2026-10-07T16:26:13.553900+00:00 | codex | CORRECTED_DSMZ618_956_BATCH_INPUTS_AND_PERCENT_ALTERNATIVES | Original PDF, preparation and exact source accession verified. Restored batch-input scope; withdrew unsupported final mass concentrations and preserved source amounts/percentages without guessing bases or final volume. Existing source-specific methylamine record reused. Prior history, IDs and unrelated metadata preserved; source-specific removed nodes remain explicit in this change event. |